Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm
In total, 117 individual samples from 39 accessions of Lactuca sativa were selected from the Czech national collection of lettuce with the aim to quantify and compare patterns of genetic and phenotypic variability within and among lettuce accessions and to propose a rapid, reliable and inexpensive m...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2019-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0004_phenotyping-and-ssr-markers-as-a-tool-for-identification-of-duplicates-in-lettuce-germplasm.php |
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| author | Michal Sochor Michaela Jemelková Ivana Doležalová |
| author_facet | Michal Sochor Michaela Jemelková Ivana Doležalová |
| author_sort | Michal Sochor |
| collection | DOAJ |
| description | In total, 117 individual samples from 39 accessions of Lactuca sativa were selected from the Czech national collection of lettuce with the aim to quantify and compare patterns of genetic and phenotypic variability within and among lettuce accessions and to propose a rapid, reliable and inexpensive method for verification of possible duplicates. We focused on phenotypic evaluation and SSR genotyping, and studied their ability to distinguish between individual accessions. Phenotypic data revealed that no two accessions shared the exactly same phenotype and no accession exhibited variability in the characters studied. Variability in SSR markers was very low as ten of twenty scorable SSR loci exhibited no variation and the remaining ten provided 48 alleles in total. Although neither phenotypic nor SSR data alone can serve as evidence for unambiguous duplicate confirmation, their combination increases the resolution power of the method considerably. The obtained data on cultivated lettuce indicate weak, but significant correlation (R2 = 0.34, P = 0.01) between the two data sets. |
| first_indexed | 2024-04-10T08:28:19Z |
| format | Article |
| id | doaj.art-52bf99def17e4bb5ad75844f1ddb39f9 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:28:19Z |
| publishDate | 2019-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-52bf99def17e4bb5ad75844f1ddb39f92023-02-23T03:30:46ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252019-09-0155311011910.17221/68/2018-CJGPBcjg-201903-0004Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasmMichal Sochor0Michaela Jemelková1Ivana DoležalováCentre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, Czech RepublicCentre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, Czech RepublicIn total, 117 individual samples from 39 accessions of Lactuca sativa were selected from the Czech national collection of lettuce with the aim to quantify and compare patterns of genetic and phenotypic variability within and among lettuce accessions and to propose a rapid, reliable and inexpensive method for verification of possible duplicates. We focused on phenotypic evaluation and SSR genotyping, and studied their ability to distinguish between individual accessions. Phenotypic data revealed that no two accessions shared the exactly same phenotype and no accession exhibited variability in the characters studied. Variability in SSR markers was very low as ten of twenty scorable SSR loci exhibited no variation and the remaining ten provided 48 alleles in total. Although neither phenotypic nor SSR data alone can serve as evidence for unambiguous duplicate confirmation, their combination increases the resolution power of the method considerably. The obtained data on cultivated lettuce indicate weak, but significant correlation (R2 = 0.34, P = 0.01) between the two data sets.https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0004_phenotyping-and-ssr-markers-as-a-tool-for-identification-of-duplicates-in-lettuce-germplasm.phpgene banksgermplasm collectionslactuca sativamicrosatellitesvariation |
| spellingShingle | Michal Sochor Michaela Jemelková Ivana Doležalová Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm Czech Journal of Genetics and Plant Breeding gene banks germplasm collections lactuca sativa microsatellites variation |
| title | Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm |
| title_full | Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm |
| title_fullStr | Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm |
| title_full_unstemmed | Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm |
| title_short | Phenotyping and SSR markers as a tool for identification of duplicates in lettuce germplasm |
| title_sort | phenotyping and ssr markers as a tool for identification of duplicates in lettuce germplasm |
| topic | gene banks germplasm collections lactuca sativa microsatellites variation |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201903-0004_phenotyping-and-ssr-markers-as-a-tool-for-identification-of-duplicates-in-lettuce-germplasm.php |
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