Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device
Introduction: Repair of peripheral nerve injuries is an intensive area of challenge and research in modern reconstructive microsurgery. Intensive research is being carried out to develop effective alternatives to the standard nerve autografting, avoiding its drawbacks. The aim of the study was to ev...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2015-10-01
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| Series: | Basic and Clinical Neuroscience |
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| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-721-2&slc_lang=en&sid=1 |
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| author | Mohamed Abdelhamid Ali Yousef Paolo Dionigi Stefania Marconi Alberto Calligaro Antonia Icaro Cornaglia Enrico Alfonsi Ferdinando Auricchio |
| author_facet | Mohamed Abdelhamid Ali Yousef Paolo Dionigi Stefania Marconi Alberto Calligaro Antonia Icaro Cornaglia Enrico Alfonsi Ferdinando Auricchio |
| author_sort | Mohamed Abdelhamid Ali Yousef |
| collection | DOAJ |
| description | Introduction: Repair of peripheral nerve injuries is an intensive area of challenge and research in
modern reconstructive microsurgery. Intensive research is being carried out to develop effective
alternatives to the standard nerve autografting, avoiding its drawbacks. The aim of the study was
to evaluate the effectiveness of a newly designed mechanical device for the reconstruction of the
sciatic nerve in rats in comparison to nerve autografting and to assess the pain during the period
of distraction neurogenesis. Methods: Fourteen Sprague Dawley rats were used and randomly assigned into 2 groups with
7 rats in each group group A (Nerve Autografting group) in which a 10-mm segment of the
sciatic nerve was resected and rotated 180 degrees, then primary end-to-end neurorrhaphy was
performed in the reverse direction group B (Nerve Lengthening group) in which the mechanical
device was inserted after surgical resection of 10 mm of the sciatic nerve, then secondary endto-
end neurorrhaphy was performed after completing the nerve lengthening. Thirteen weeks
later, assessment of the functional sciatic nerve recovery using static sciatic index (SSI) was
performed. Furthermore, fourteen weeks after the nerve resection, assessment of the nerve
regeneration with electrophysiological study and histological analysis were performed. Also,
gastrocnemius wet weight was measured. For pain assessment in group B, Rat Grimace Scale
(RGS) score was used. Results: Significantly better functional recovery rate (using the SSI) was reported in the nerve
lengthening group in comparison to autografting group. Also, a statistically significant higher
nerve conduction velocity was detected in the nerve lengthening group. On histological analysis
of the distal nerve section at 3 mm distal to the nerve repair site, significant myelin sheath
thickness was detected in the nerve lengthening group. Discussion: Distraction neurogenesis with the new experimental device is a reliable therapeutic
method for the reconstruction of nerve defects. |
| first_indexed | 2024-03-07T17:25:12Z |
| format | Article |
| id | doaj.art-52c103331f334272ae36df45bb5c8071 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:25:12Z |
| publishDate | 2015-10-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-52c103331f334272ae36df45bb5c80712024-03-02T19:16:38ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422015-10-0164253264Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental DeviceMohamed Abdelhamid Ali Yousef0Paolo Dionigi1Stefania Marconi2Alberto Calligaro3Antonia Icaro Cornaglia4Enrico Alfonsi5Ferdinando Auricchio6 Experimental Surgery and Microsurgery Research Unit, Department of General Surgery, University of Pavia, V.le Golgi 19, 27100, Pavia, Italy. Organization Organization Organization Organization Organization Organization Introduction: Repair of peripheral nerve injuries is an intensive area of challenge and research in modern reconstructive microsurgery. Intensive research is being carried out to develop effective alternatives to the standard nerve autografting, avoiding its drawbacks. The aim of the study was to evaluate the effectiveness of a newly designed mechanical device for the reconstruction of the sciatic nerve in rats in comparison to nerve autografting and to assess the pain during the period of distraction neurogenesis. Methods: Fourteen Sprague Dawley rats were used and randomly assigned into 2 groups with 7 rats in each group group A (Nerve Autografting group) in which a 10-mm segment of the sciatic nerve was resected and rotated 180 degrees, then primary end-to-end neurorrhaphy was performed in the reverse direction group B (Nerve Lengthening group) in which the mechanical device was inserted after surgical resection of 10 mm of the sciatic nerve, then secondary endto- end neurorrhaphy was performed after completing the nerve lengthening. Thirteen weeks later, assessment of the functional sciatic nerve recovery using static sciatic index (SSI) was performed. Furthermore, fourteen weeks after the nerve resection, assessment of the nerve regeneration with electrophysiological study and histological analysis were performed. Also, gastrocnemius wet weight was measured. For pain assessment in group B, Rat Grimace Scale (RGS) score was used. Results: Significantly better functional recovery rate (using the SSI) was reported in the nerve lengthening group in comparison to autografting group. Also, a statistically significant higher nerve conduction velocity was detected in the nerve lengthening group. On histological analysis of the distal nerve section at 3 mm distal to the nerve repair site, significant myelin sheath thickness was detected in the nerve lengthening group. Discussion: Distraction neurogenesis with the new experimental device is a reliable therapeutic method for the reconstruction of nerve defects.http://bcn.iums.ac.ir/browse.php?a_code=A-10-721-2&slc_lang=en&sid=1Nerve lengthening Peripheral nerve injuries Nerve regeneration |
| spellingShingle | Mohamed Abdelhamid Ali Yousef Paolo Dionigi Stefania Marconi Alberto Calligaro Antonia Icaro Cornaglia Enrico Alfonsi Ferdinando Auricchio Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device Basic and Clinical Neuroscience Nerve lengthening Peripheral nerve injuries Nerve regeneration |
| title | Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device |
| title_full | Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device |
| title_fullStr | Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device |
| title_full_unstemmed | Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device |
| title_short | Successful Reconstruction of Nerve Defects Using Distraction Neurogenesis with a New Experimental Device |
| title_sort | successful reconstruction of nerve defects using distraction neurogenesis with a new experimental device |
| topic | Nerve lengthening Peripheral nerve injuries Nerve regeneration |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-721-2&slc_lang=en&sid=1 |
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