Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates
The vaginal microbiome of healthy women contains nondiphtheria corynebacteria. The role and functions of nondiphtheria corynebacteria in the vaginal biotope are still under study. We sequenced and analysed the genomes of three vaginal <i>C. amycolatum</i> strains isolated from healthy wo...
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/249 |
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| author | Irina V. Gladysheva Sergey V. Cherkasov Yuriy A. Khlopko Andrey O. Plotnikov |
| author_facet | Irina V. Gladysheva Sergey V. Cherkasov Yuriy A. Khlopko Andrey O. Plotnikov |
| author_sort | Irina V. Gladysheva |
| collection | DOAJ |
| description | The vaginal microbiome of healthy women contains nondiphtheria corynebacteria. The role and functions of nondiphtheria corynebacteria in the vaginal biotope are still under study. We sequenced and analysed the genomes of three vaginal <i>C. amycolatum</i> strains isolated from healthy women. Previous studies have shown that these strains produced metabolites that significantly increased the antagonistic activity of peroxide-producing lactic acid bacteria against pathogenic and opportunistic microorganisms and had strong antimicrobial activity against opportunistic pathogens. Analysis of the <i>C. amycolatum</i> genomes revealed the genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genes responsible for the production of H<sub>2</sub>O<sub>2</sub> and the synthesis of secondary metabolites, essential amino acids and vitamins were identified. A cluster of genes encoding the synthesis of bacteriocin was revealed in one of the annotated genomes. The obtained results allow us to consider the studied strains as potential probiotics that are capable of preventing the growth of pathogenic microorganisms and supporting colonisation resistance in the vaginal biotope. |
| first_indexed | 2024-03-09T21:24:35Z |
| format | Article |
| id | doaj.art-52c1c58d3ce84608bf0ff90a851f3a43 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:24:35Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-52c1c58d3ce84608bf0ff90a851f3a432023-11-23T21:13:12ZengMDPI AGMicroorganisms2076-26072022-01-0110224910.3390/microorganisms10020249Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal IsolatesIrina V. Gladysheva0Sergey V. Cherkasov1Yuriy A. Khlopko2Andrey O. Plotnikov3Institute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaInstitute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaInstitute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaInstitute of Cellular and Intracellular Symbiosis, Ural Branch of the Russian Academy of Sciences, 460000 Orenburg, RussiaThe vaginal microbiome of healthy women contains nondiphtheria corynebacteria. The role and functions of nondiphtheria corynebacteria in the vaginal biotope are still under study. We sequenced and analysed the genomes of three vaginal <i>C. amycolatum</i> strains isolated from healthy women. Previous studies have shown that these strains produced metabolites that significantly increased the antagonistic activity of peroxide-producing lactic acid bacteria against pathogenic and opportunistic microorganisms and had strong antimicrobial activity against opportunistic pathogens. Analysis of the <i>C. amycolatum</i> genomes revealed the genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genes responsible for the production of H<sub>2</sub>O<sub>2</sub> and the synthesis of secondary metabolites, essential amino acids and vitamins were identified. A cluster of genes encoding the synthesis of bacteriocin was revealed in one of the annotated genomes. The obtained results allow us to consider the studied strains as potential probiotics that are capable of preventing the growth of pathogenic microorganisms and supporting colonisation resistance in the vaginal biotope.https://www.mdpi.com/2076-2607/10/2/249<i>Corynebacterium amycolatum</i>vaginal microbiomegenomesecondary metabolismbacteriocin |
| spellingShingle | Irina V. Gladysheva Sergey V. Cherkasov Yuriy A. Khlopko Andrey O. Plotnikov Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates Microorganisms <i>Corynebacterium amycolatum</i> vaginal microbiome genome secondary metabolism bacteriocin |
| title | Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates |
| title_full | Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates |
| title_fullStr | Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates |
| title_full_unstemmed | Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates |
| title_short | Genome Characterization and Probiotic Potential of <i>Corynebacterium amycolatum</i> Human Vaginal Isolates |
| title_sort | genome characterization and probiotic potential of i corynebacterium amycolatum i human vaginal isolates |
| topic | <i>Corynebacterium amycolatum</i> vaginal microbiome genome secondary metabolism bacteriocin |
| url | https://www.mdpi.com/2076-2607/10/2/249 |
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