Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions.
Plant growth-promoting microbes (PGPM) play vital roles in maintaining crop fitness and soil health in stressed environments. Research have included analysis-based cultivation of soil-microbial-plant relationships to clarify microbiota potential. The goal of the research was to (i) evaluate the symb...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0259380 |
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| author | Robert Pokluda Lucia Ragasová Miloš Jurica Andrzej Kalisz Monika Komorowska Marcin Niemiec Agnieszka Sekara |
| author_facet | Robert Pokluda Lucia Ragasová Miloš Jurica Andrzej Kalisz Monika Komorowska Marcin Niemiec Agnieszka Sekara |
| author_sort | Robert Pokluda |
| collection | DOAJ |
| description | Plant growth-promoting microbes (PGPM) play vital roles in maintaining crop fitness and soil health in stressed environments. Research have included analysis-based cultivation of soil-microbial-plant relationships to clarify microbiota potential. The goal of the research was to (i) evaluate the symbiotic microorganism effects on tomato seedling fitness under stressed conditions simulating a fragile soil susceptible to degradation; (ii) compare the plant-microbial interactions after inoculation with microbial isolates and fungi-bacteria consortia; (iii) develop an effective crop-microbial network, which improves soil and plant status. The experimental design included non-inoculated treatments with peat and sand at ratios of 50:50, 70:30, 100:0 (v:v), inoculated treatments with arbuscular mycorrhizal fungi (AMF) and Azospirillum brasilense (AZ) using the aforementioned peat:sand ratios; and treatment with peat co-inoculated with AMF and Saccharothrix tamanrassetensis (S). AMF + AZ increased root fresh weight in peat substrate compared to the control (4.4 to 3.3 g plant-1). An increase in shoot fresh weight was detected in the AMF + AZ treatment with a 50:50 peat:sand ratio (10.1 to 8.5 g plant-1). AMF + AZ reduced antioxidant activity (DPPH) (18-34%) in leaves, whereas AMF + S had the highest DPPH in leaves and roots (45%). Total leaf phenolic content was higher in control with a decreased proportion of peat. Peroxidase activity was enhanced in AMF + AZ and AMF + S treatments, except for AMF + AZ in peat. Microscopic root assays revealed the ability of AMF to establish strong fungal-tomato symbiosis; the colonization rate was 78-89%. AMF + AZ accelerated K and Mg accumulation in tomato leaves in treatments reflecting soil stress. To date, there has been no relevant information regarding the successful AMF and Saccharothrix co-inoculation relationship. This study confirmed that AMF + S could increase the P, S, and Fe status of seedlings under high organic C content conditions. The improved tomato growth and nutrient acquisition demonstrated the potential of PGPM colonization under degraded soil conditions. |
| first_indexed | 2024-04-11T20:16:58Z |
| format | Article |
| id | doaj.art-52c3575851ad49d4b4cf72d8c2d62515 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T20:16:58Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-52c3575851ad49d4b4cf72d8c2d625152022-12-22T04:04:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011611e025938010.1371/journal.pone.0259380Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions.Robert PokludaLucia RagasováMiloš JuricaAndrzej KaliszMonika KomorowskaMarcin NiemiecAgnieszka SekaraPlant growth-promoting microbes (PGPM) play vital roles in maintaining crop fitness and soil health in stressed environments. Research have included analysis-based cultivation of soil-microbial-plant relationships to clarify microbiota potential. The goal of the research was to (i) evaluate the symbiotic microorganism effects on tomato seedling fitness under stressed conditions simulating a fragile soil susceptible to degradation; (ii) compare the plant-microbial interactions after inoculation with microbial isolates and fungi-bacteria consortia; (iii) develop an effective crop-microbial network, which improves soil and plant status. The experimental design included non-inoculated treatments with peat and sand at ratios of 50:50, 70:30, 100:0 (v:v), inoculated treatments with arbuscular mycorrhizal fungi (AMF) and Azospirillum brasilense (AZ) using the aforementioned peat:sand ratios; and treatment with peat co-inoculated with AMF and Saccharothrix tamanrassetensis (S). AMF + AZ increased root fresh weight in peat substrate compared to the control (4.4 to 3.3 g plant-1). An increase in shoot fresh weight was detected in the AMF + AZ treatment with a 50:50 peat:sand ratio (10.1 to 8.5 g plant-1). AMF + AZ reduced antioxidant activity (DPPH) (18-34%) in leaves, whereas AMF + S had the highest DPPH in leaves and roots (45%). Total leaf phenolic content was higher in control with a decreased proportion of peat. Peroxidase activity was enhanced in AMF + AZ and AMF + S treatments, except for AMF + AZ in peat. Microscopic root assays revealed the ability of AMF to establish strong fungal-tomato symbiosis; the colonization rate was 78-89%. AMF + AZ accelerated K and Mg accumulation in tomato leaves in treatments reflecting soil stress. To date, there has been no relevant information regarding the successful AMF and Saccharothrix co-inoculation relationship. This study confirmed that AMF + S could increase the P, S, and Fe status of seedlings under high organic C content conditions. The improved tomato growth and nutrient acquisition demonstrated the potential of PGPM colonization under degraded soil conditions.https://doi.org/10.1371/journal.pone.0259380 |
| spellingShingle | Robert Pokluda Lucia Ragasová Miloš Jurica Andrzej Kalisz Monika Komorowska Marcin Niemiec Agnieszka Sekara Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. PLoS ONE |
| title | Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. |
| title_full | Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. |
| title_fullStr | Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. |
| title_full_unstemmed | Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. |
| title_short | Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions. |
| title_sort | effects of growth promoting microorganisms on tomato seedlings growing in different media conditions |
| url | https://doi.org/10.1371/journal.pone.0259380 |
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