Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host
Bacterial proteomic studies frequently use strains cultured in synthetic liquid media over many generations. It is uncertain whether bacterial proteins expressed under these conditions will be the same as the repertoire found in natural environments, or when bacteria are infecting a host organism. T...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00071/full |
| _version_ | 1798043348625260544 |
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| author | Megan Anne Rees Timothy P Stinear Robert eGoode Ross Leon Coppel Alexander Ian Smith Oded eKleifeld |
| author_facet | Megan Anne Rees Timothy P Stinear Robert eGoode Ross Leon Coppel Alexander Ian Smith Oded eKleifeld |
| author_sort | Megan Anne Rees |
| collection | DOAJ |
| description | Bacterial proteomic studies frequently use strains cultured in synthetic liquid media over many generations. It is uncertain whether bacterial proteins expressed under these conditions will be the same as the repertoire found in natural environments, or when bacteria are infecting a host organism. Thus, genomic and proteomic characterisation of bacteria derived from the host environment in comparison to reference strains grown in the lab, should aid understanding of pathogenesis. Isolates of Corynebacterium pseudotuberculosis were obtained from the nodes of three naturally infected sheep and compared to a laboratory reference strain using bottom-up proteomics, after whole genome sequencing of each of the field isolates. These comparisons were performed following growth in liquid media that allowed us to reach the required protein amount for proteomic analysis. Over 1350 proteins identified in the isolated strains, from which unique proteome features were revealed. Several of the identified proteins demonstrated a significant abundance difference in the field isolates compared to the reference strain even though there were no obvious differences in the DNA sequence of the corresponding gene or in nearby non-coding DNA. Higher abundance in the field isolates was observed for proteins related to hypoxia and nutrient deficiency responses as well as to thiopeptide biosynthesis. |
| first_indexed | 2024-04-11T22:47:52Z |
| format | Article |
| id | doaj.art-52c4626f74e44d2aae9a04d0ff520339 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T22:47:52Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-52c4626f74e44d2aae9a04d0ff5203392022-12-22T03:58:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882015-10-01510.3389/fcimb.2015.00071146411Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural HostMegan Anne Rees0Timothy P Stinear1Robert eGoode2Ross Leon Coppel3Alexander Ian Smith4Oded eKleifeld5Monash UniversityUniversity of MelbourneMonash UniversityMonash UniversityMonash UniversityMonash UniversityBacterial proteomic studies frequently use strains cultured in synthetic liquid media over many generations. It is uncertain whether bacterial proteins expressed under these conditions will be the same as the repertoire found in natural environments, or when bacteria are infecting a host organism. Thus, genomic and proteomic characterisation of bacteria derived from the host environment in comparison to reference strains grown in the lab, should aid understanding of pathogenesis. Isolates of Corynebacterium pseudotuberculosis were obtained from the nodes of three naturally infected sheep and compared to a laboratory reference strain using bottom-up proteomics, after whole genome sequencing of each of the field isolates. These comparisons were performed following growth in liquid media that allowed us to reach the required protein amount for proteomic analysis. Over 1350 proteins identified in the isolated strains, from which unique proteome features were revealed. Several of the identified proteins demonstrated a significant abundance difference in the field isolates compared to the reference strain even though there were no obvious differences in the DNA sequence of the corresponding gene or in nearby non-coding DNA. Higher abundance in the field isolates was observed for proteins related to hypoxia and nutrient deficiency responses as well as to thiopeptide biosynthesis.http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00071/fullCorynebacterium pseudotuberculosisLymph Nodescobalaminnatural infectionPrecorrinThiopeptide |
| spellingShingle | Megan Anne Rees Timothy P Stinear Robert eGoode Ross Leon Coppel Alexander Ian Smith Oded eKleifeld Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host Frontiers in Cellular and Infection Microbiology Corynebacterium pseudotuberculosis Lymph Nodes cobalamin natural infection Precorrin Thiopeptide |
| title | Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host |
| title_full | Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host |
| title_fullStr | Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host |
| title_full_unstemmed | Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host |
| title_short | Changes In Protein Abundance Are Observed In Bacterial Isolates from a Natural Host |
| title_sort | changes in protein abundance are observed in bacterial isolates from a natural host |
| topic | Corynebacterium pseudotuberculosis Lymph Nodes cobalamin natural infection Precorrin Thiopeptide |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00071/full |
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