Use of a saline gradient for the diagnosis of schistosomiasis
The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2009-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500010 |
| _version_ | 1797718355805732864 |
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| author | Paulo Marcos Z Coelho Arnon D Jurberg Áureo A Oliveira Naftale Katz |
| author_facet | Paulo Marcos Z Coelho Arnon D Jurberg Áureo A Oliveira Naftale Katz |
| author_sort | Paulo Marcos Z Coelho |
| collection | DOAJ |
| description | The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3% saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a signifi-cantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10% formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences. |
| first_indexed | 2024-03-12T08:49:50Z |
| format | Article |
| id | doaj.art-52c4e840e4934bd2a8c5ffc6539babc6 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:50Z |
| publishDate | 2009-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-52c4e840e4934bd2a8c5ffc6539babc62023-09-02T16:22:01ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-08-01104572072310.1590/S0074-02762009000500010Use of a saline gradient for the diagnosis of schistosomiasisPaulo Marcos Z CoelhoArnon D JurbergÁureo A OliveiraNaftale KatzThe development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3% saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a signifi-cantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10% formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500010Schistosoma mansonidiagnosisfaeces |
| spellingShingle | Paulo Marcos Z Coelho Arnon D Jurberg Áureo A Oliveira Naftale Katz Use of a saline gradient for the diagnosis of schistosomiasis Memorias do Instituto Oswaldo Cruz Schistosoma mansoni diagnosis faeces |
| title | Use of a saline gradient for the diagnosis of schistosomiasis |
| title_full | Use of a saline gradient for the diagnosis of schistosomiasis |
| title_fullStr | Use of a saline gradient for the diagnosis of schistosomiasis |
| title_full_unstemmed | Use of a saline gradient for the diagnosis of schistosomiasis |
| title_short | Use of a saline gradient for the diagnosis of schistosomiasis |
| title_sort | use of a saline gradient for the diagnosis of schistosomiasis |
| topic | Schistosoma mansoni diagnosis faeces |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500010 |
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