Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS)
<p>Abstract</p> <p>Background</p> <p>Polycystic Ovary Syndrome (PCOS) is an endocrine-metabolic disorder commonly associated with insulin resistance (IR). Previous studies indicate about the expression of molecules involved in the insulin pathway in endometria of women...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://www.rbej.com/content/10/1/17 |
| _version_ | 1818491558895288320 |
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| author | Rivero Rodrigo Garin Claire-Alix Ormazabal Paulina Silva Andrea Carvajal Rodrigo Gabler Fernando Romero Carmen Vega Margarita |
| author_facet | Rivero Rodrigo Garin Claire-Alix Ormazabal Paulina Silva Andrea Carvajal Rodrigo Gabler Fernando Romero Carmen Vega Margarita |
| author_sort | Rivero Rodrigo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Polycystic Ovary Syndrome (PCOS) is an endocrine-metabolic disorder commonly associated with insulin resistance (IR). Previous studies indicate about the expression of molecules involved in the insulin pathway in endometria of women with PCOS-IR. Therefore, the aim of the present study was to evaluate the effect of insulin and testosterone in the expression of these proteins in the endometria and immortal endometrial stromal cell line (T-HESCs).</p> <p>Methods</p> <p>We examined the protein levels of Munc18c, PKC zeta, phospho-PKC Zeta, and Syntaxin-4. Protein levels were assessed by Western Blot and/or immunohistochemistry in proliferative endometria (NPE = 6) and in PCOS endometria with insulin resistance (PCOSE-IR = 6). We also evaluated whether high concentrations of insulin (100 nM) and/or testosterone (100 nM), during a 24 h stimulatory period, affected the expression of these proteins in an immortal endometrial stromal cell line (T-HESCs). Once stimulated, proteins were extracted from cells and were assessed by Western Blot analysis. Immunocytochemistry was performed to detect AR in T-HESC cells.</p> <p>Results</p> <p>Western Blot data showed decreased expression (<it>p </it>< 0,05) of Munc18c and phospho-PKC Zeta in PCOS-IR endometria (PCOSE-IR) with respect to the control (NPE). In the <it>in vitro </it>study, Western Blot analysis showed decreased levels of Munc18c, PKC Zeta and phospho-PKC Zeta with the different hormonal treatments when compared to the control condition (no hormonal stimulation) (<it>p </it>< 0,05). The AR was present in the endometrial stromal cell line (T-HESC).</p> <p>Conclusion</p> <p>The conditions of hyperinsulinism and hyperandrogenism present in PCOS-IR patients modulate the expression and/or phosphorylation of the proteins involved in the insulin pathway at the endometrial level. These data extend to the T-HESCs cells results, where insulin and testosterone exert an effect on both the expression and phosphorylation of proteins present in the pathway.</p> |
| first_indexed | 2024-12-10T17:32:37Z |
| format | Article |
| id | doaj.art-52c7afc3199b41a79dd34aa2138a9b6b |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-10T17:32:37Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-52c7afc3199b41a79dd34aa2138a9b6b2022-12-22T01:39:37ZengBMCReproductive Biology and Endocrinology1477-78272012-03-011011710.1186/1477-7827-10-17Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS)Rivero RodrigoGarin Claire-AlixOrmazabal PaulinaSilva AndreaCarvajal RodrigoGabler FernandoRomero CarmenVega Margarita<p>Abstract</p> <p>Background</p> <p>Polycystic Ovary Syndrome (PCOS) is an endocrine-metabolic disorder commonly associated with insulin resistance (IR). Previous studies indicate about the expression of molecules involved in the insulin pathway in endometria of women with PCOS-IR. Therefore, the aim of the present study was to evaluate the effect of insulin and testosterone in the expression of these proteins in the endometria and immortal endometrial stromal cell line (T-HESCs).</p> <p>Methods</p> <p>We examined the protein levels of Munc18c, PKC zeta, phospho-PKC Zeta, and Syntaxin-4. Protein levels were assessed by Western Blot and/or immunohistochemistry in proliferative endometria (NPE = 6) and in PCOS endometria with insulin resistance (PCOSE-IR = 6). We also evaluated whether high concentrations of insulin (100 nM) and/or testosterone (100 nM), during a 24 h stimulatory period, affected the expression of these proteins in an immortal endometrial stromal cell line (T-HESCs). Once stimulated, proteins were extracted from cells and were assessed by Western Blot analysis. Immunocytochemistry was performed to detect AR in T-HESC cells.</p> <p>Results</p> <p>Western Blot data showed decreased expression (<it>p </it>< 0,05) of Munc18c and phospho-PKC Zeta in PCOS-IR endometria (PCOSE-IR) with respect to the control (NPE). In the <it>in vitro </it>study, Western Blot analysis showed decreased levels of Munc18c, PKC Zeta and phospho-PKC Zeta with the different hormonal treatments when compared to the control condition (no hormonal stimulation) (<it>p </it>< 0,05). The AR was present in the endometrial stromal cell line (T-HESC).</p> <p>Conclusion</p> <p>The conditions of hyperinsulinism and hyperandrogenism present in PCOS-IR patients modulate the expression and/or phosphorylation of the proteins involved in the insulin pathway at the endometrial level. These data extend to the T-HESCs cells results, where insulin and testosterone exert an effect on both the expression and phosphorylation of proteins present in the pathway.</p>http://www.rbej.com/content/10/1/17PKC ZetaMunc18cEndometriumPCOS |
| spellingShingle | Rivero Rodrigo Garin Claire-Alix Ormazabal Paulina Silva Andrea Carvajal Rodrigo Gabler Fernando Romero Carmen Vega Margarita Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) Reproductive Biology and Endocrinology PKC Zeta Munc18c Endometrium PCOS |
| title | Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) |
| title_full | Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) |
| title_fullStr | Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) |
| title_full_unstemmed | Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) |
| title_short | Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) |
| title_sort | protein expression of pkcz protein kinase c zeta munc18c and syntaxin 4 in the insulin pathway in endometria of patients with polycystic ovary syndrome pcos |
| topic | PKC Zeta Munc18c Endometrium PCOS |
| url | http://www.rbej.com/content/10/1/17 |
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