Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction
Myocardium is an excitable tissue with electrical conductivity and mechanical strength. In this work, carbon fibers (CFs) and co-axial fibrous mesh were integrated which combined the high modulus and excellent electrical conductivity of CFs and the fibrous and porous structures of the electrospun fi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422002137 |
| _version_ | 1797990847433670656 |
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| author | Jie Meng Bo Xiao Fengxin Wu Lihong Sun Bo Li Wen Guo Xuechun Hu Xuegai Xu Tao Wen Jian Liu Haiyan Xu |
| author_facet | Jie Meng Bo Xiao Fengxin Wu Lihong Sun Bo Li Wen Guo Xuechun Hu Xuegai Xu Tao Wen Jian Liu Haiyan Xu |
| author_sort | Jie Meng |
| collection | DOAJ |
| description | Myocardium is an excitable tissue with electrical conductivity and mechanical strength. In this work, carbon fibers (CFs) and co-axial fibrous mesh were integrated which combined the high modulus and excellent electrical conductivity of CFs and the fibrous and porous structures of the electrospun fibers. The scaffold was fabricated by simply integrating coaxial electrospun fibers and carbon fibers through a freeze-drying procedure. It was shown that the integration of carbon fibers have the conductivity and Young's modulus of the fibrous mesh increased significantly, meanwhile, upregulated the expression of CX43, α-actinin, RhoA of the neonatal rat primary cardiomyocytes and primary human umbilical vein endothelial cells (HUVECs), and promoted the secretion of VEGF of HUVECs. Moreover, the cardiomyocytes grown on the scaffolds increased the ability of HUVECs migration. When implanted to the injury area post myocardial infraction, the scaffolds were able to effectively enhance the tissue regeneration and new vessel formation, which rescued the heart dysfunction induced by the myocardial infraction, evidenced by the results of echocardiography and histochemical analysis. In conclusion, the composite scaffolds could promote the myocardium regeneration and function's recovery by enhancing cardiomyocytes maturation and angiogenesis and establishing the crosstalk between the cardiomyocytes and the vascular endothelial cells. |
| first_indexed | 2024-04-11T08:41:57Z |
| format | Article |
| id | doaj.art-52cabf055f554aaabc9aa8bdbd03a299 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-11T08:41:57Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-52cabf055f554aaabc9aa8bdbd03a2992022-12-22T04:34:08ZengElsevierMaterials Today Bio2590-00642022-12-0116100415Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarctionJie Meng0Bo Xiao1Fengxin Wu2Lihong Sun3Bo Li4Wen Guo5Xuechun Hu6Xuegai Xu7Tao Wen8Jian Liu9Haiyan Xu10Department of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaDepartment of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, ChinaDepartment of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaCenter for Experimental Animal Research, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaPeking Union Medical College, Beijing, 100730, ChinaPeking Union Medical College, Beijing, 100730, ChinaDepartment of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaDepartment of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaDepartment of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, ChinaDepartment of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China; Corresponding author.Department of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China; Corresponding author.Myocardium is an excitable tissue with electrical conductivity and mechanical strength. In this work, carbon fibers (CFs) and co-axial fibrous mesh were integrated which combined the high modulus and excellent electrical conductivity of CFs and the fibrous and porous structures of the electrospun fibers. The scaffold was fabricated by simply integrating coaxial electrospun fibers and carbon fibers through a freeze-drying procedure. It was shown that the integration of carbon fibers have the conductivity and Young's modulus of the fibrous mesh increased significantly, meanwhile, upregulated the expression of CX43, α-actinin, RhoA of the neonatal rat primary cardiomyocytes and primary human umbilical vein endothelial cells (HUVECs), and promoted the secretion of VEGF of HUVECs. Moreover, the cardiomyocytes grown on the scaffolds increased the ability of HUVECs migration. When implanted to the injury area post myocardial infraction, the scaffolds were able to effectively enhance the tissue regeneration and new vessel formation, which rescued the heart dysfunction induced by the myocardial infraction, evidenced by the results of echocardiography and histochemical analysis. In conclusion, the composite scaffolds could promote the myocardium regeneration and function's recovery by enhancing cardiomyocytes maturation and angiogenesis and establishing the crosstalk between the cardiomyocytes and the vascular endothelial cells.http://www.sciencedirect.com/science/article/pii/S2590006422002137Conductive ScaffoldCarbon fibersMyocardiumRegenerationVascularization |
| spellingShingle | Jie Meng Bo Xiao Fengxin Wu Lihong Sun Bo Li Wen Guo Xuechun Hu Xuegai Xu Tao Wen Jian Liu Haiyan Xu Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction Materials Today Bio Conductive Scaffold Carbon fibers Myocardium Regeneration Vascularization |
| title | Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| title_full | Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| title_fullStr | Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| title_full_unstemmed | Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| title_short | Co-axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| title_sort | co axial fibrous scaffolds integrating with carbon fiber promote cardiac tissue regeneration post myocardial infarction |
| topic | Conductive Scaffold Carbon fibers Myocardium Regeneration Vascularization |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422002137 |
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