Effect of antibiotics on callus regeneration during transformation of IR 64 rice
We report here the effect of antibiotics on the regeneration potential of recalcitrant indica rice cultivar, IR64. Different protocols reporting high-efficiency agro-bacterium-mediated transformation of mature seed-derived regenerative calli were used and compared. The putative transgenic (T0) plant...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000399 |
| _version_ | 1811341157259542528 |
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| author | Thanh Ngoc Tran Neeti Sanan-Mishra |
| author_facet | Thanh Ngoc Tran Neeti Sanan-Mishra |
| author_sort | Thanh Ngoc Tran |
| collection | DOAJ |
| description | We report here the effect of antibiotics on the regeneration potential of recalcitrant indica rice cultivar, IR64. Different protocols reporting high-efficiency agro-bacterium-mediated transformation of mature seed-derived regenerative calli were used and compared. The putative transgenic (T0) plants were analyzed for integration of the transgene through polymerase chain reaction and Southern blotting analyses. It was observed that the high-efficiency transformation of scutellar-derived regenerative calli could be obtained by using maltose as a carbon source and increased quantity of 2,4-D on a medium containing a higher concentration of gelling agent. The percentage of regeneration is greatly affected by the presence of antibiotics. |
| first_indexed | 2024-04-13T18:52:40Z |
| format | Article |
| id | doaj.art-52cafa22a4c94572b7bd54873e338ed8 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T18:52:40Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-52cafa22a4c94572b7bd54873e338ed82022-12-22T02:34:21ZengElsevierBiotechnology Reports2215-017X2015-09-017C14314910.1016/j.btre.2015.06.004Effect of antibiotics on callus regeneration during transformation of IR 64 riceThanh Ngoc Tran0Neeti Sanan-Mishra1Plant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, IndiaPlant Molecular Biology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, IndiaWe report here the effect of antibiotics on the regeneration potential of recalcitrant indica rice cultivar, IR64. Different protocols reporting high-efficiency agro-bacterium-mediated transformation of mature seed-derived regenerative calli were used and compared. The putative transgenic (T0) plants were analyzed for integration of the transgene through polymerase chain reaction and Southern blotting analyses. It was observed that the high-efficiency transformation of scutellar-derived regenerative calli could be obtained by using maltose as a carbon source and increased quantity of 2,4-D on a medium containing a higher concentration of gelling agent. The percentage of regeneration is greatly affected by the presence of antibiotics.http://www.sciencedirect.com/science/article/pii/S2215017X15000399AntibioticsRegenerationRiceIR64Agro-bacterium |
| spellingShingle | Thanh Ngoc Tran Neeti Sanan-Mishra Effect of antibiotics on callus regeneration during transformation of IR 64 rice Biotechnology Reports Antibiotics Regeneration Rice IR64 Agro-bacterium |
| title | Effect of antibiotics on callus regeneration during transformation of IR 64 rice |
| title_full | Effect of antibiotics on callus regeneration during transformation of IR 64 rice |
| title_fullStr | Effect of antibiotics on callus regeneration during transformation of IR 64 rice |
| title_full_unstemmed | Effect of antibiotics on callus regeneration during transformation of IR 64 rice |
| title_short | Effect of antibiotics on callus regeneration during transformation of IR 64 rice |
| title_sort | effect of antibiotics on callus regeneration during transformation of ir 64 rice |
| topic | Antibiotics Regeneration Rice IR64 Agro-bacterium |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X15000399 |
| work_keys_str_mv | AT thanhngoctran effectofantibioticsoncallusregenerationduringtransformationofir64rice AT neetisananmishra effectofantibioticsoncallusregenerationduringtransformationofir64rice |