Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice
Low oxygen concentrations in flooded paddy fields cause poor seedling establishment in wet direct seeded rice. We aimed to identify novel genomic regions associated with coleoptile elongation under anaerobic conditions in order to improve seedling establishment. Chromosome segment substitution lines...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Plant Production Science |
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| Online Access: | http://dx.doi.org/10.1080/1343943X.2020.1740600 |
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| author | Tomio Nishimura Kazuhiro Sasaki Takuya Yamaguchi Hirokazu Takahashi Junko Yamagishi Yoichiro Kato |
| author_facet | Tomio Nishimura Kazuhiro Sasaki Takuya Yamaguchi Hirokazu Takahashi Junko Yamagishi Yoichiro Kato |
| author_sort | Tomio Nishimura |
| collection | DOAJ |
| description | Low oxygen concentrations in flooded paddy fields cause poor seedling establishment in wet direct seeded rice. We aimed to identify novel genomic regions associated with coleoptile elongation under anaerobic conditions in order to improve seedling establishment. Chromosome segment substitution lines (CSSLs), substituted with Koshihikari (Japonica-type) in the IR 64 genetic background (Indica-type) were evaluated. These lines were imbibed with a hydroponic solution containing Oxyrase to create a stable anaerobic condition and coleoptile lengths were measured six days after incubation at 30°C. Among the CSSLs, only SL2111 had a significantly longer coleoptile (23.9 mm) than that of IR 64 (14.3 mm). From genotype and phenotype analyses, a novel QTL, referred to as qACE3.1, for coleoptile elongation under anaerobic conditions was detected on chromosome 3. To explore the molecular mechanism of qACE3.1, the expression levels of genes encoding enzymes involved in starch degradation and fermentation were assessed. In SL2111 coleoptiles, the expression levels of pyruvate decarboxylase were significantly higher than in IR 64 coleoptiles whereas those of alcohol dehydrogenase were lower. In contrast, no differences were observed in the expression levels of genes associated with starch degradation. These results imply that qACE3.1 may specifically affect fermentative metabolism. In addition, we evaluated the impact of qACE3.1 on seedling establishment under flooded conditions in a paddy field. |
| first_indexed | 2024-12-11T01:41:16Z |
| format | Article |
| id | doaj.art-52cc40200cf346d385b5a3586a461e87 |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-11T01:41:16Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-52cc40200cf346d385b5a3586a461e872022-12-22T01:25:02ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082020-07-0123337438310.1080/1343943X.2020.17406001740600Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in riceTomio Nishimura0Kazuhiro Sasaki1Takuya Yamaguchi2Hirokazu Takahashi3Junko Yamagishi4Yoichiro Kato5The University of TokyoThe University of TokyoToyama Prefectural Agricultural, Forestry & Fisheries Research CenterNagoya UniversityThe University of TokyoThe University of TokyoLow oxygen concentrations in flooded paddy fields cause poor seedling establishment in wet direct seeded rice. We aimed to identify novel genomic regions associated with coleoptile elongation under anaerobic conditions in order to improve seedling establishment. Chromosome segment substitution lines (CSSLs), substituted with Koshihikari (Japonica-type) in the IR 64 genetic background (Indica-type) were evaluated. These lines were imbibed with a hydroponic solution containing Oxyrase to create a stable anaerobic condition and coleoptile lengths were measured six days after incubation at 30°C. Among the CSSLs, only SL2111 had a significantly longer coleoptile (23.9 mm) than that of IR 64 (14.3 mm). From genotype and phenotype analyses, a novel QTL, referred to as qACE3.1, for coleoptile elongation under anaerobic conditions was detected on chromosome 3. To explore the molecular mechanism of qACE3.1, the expression levels of genes encoding enzymes involved in starch degradation and fermentation were assessed. In SL2111 coleoptiles, the expression levels of pyruvate decarboxylase were significantly higher than in IR 64 coleoptiles whereas those of alcohol dehydrogenase were lower. In contrast, no differences were observed in the expression levels of genes associated with starch degradation. These results imply that qACE3.1 may specifically affect fermentative metabolism. In addition, we evaluated the impact of qACE3.1 on seedling establishment under flooded conditions in a paddy field.http://dx.doi.org/10.1080/1343943X.2020.1740600anaerobic germinationcoleoptile elongationdirect-seeded riceflooding stressoxyrasequantitative trait locus |
| spellingShingle | Tomio Nishimura Kazuhiro Sasaki Takuya Yamaguchi Hirokazu Takahashi Junko Yamagishi Yoichiro Kato Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice Plant Production Science anaerobic germination coleoptile elongation direct-seeded rice flooding stress oxyrase quantitative trait locus |
| title | Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| title_full | Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| title_fullStr | Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| title_full_unstemmed | Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| title_short | Detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| title_sort | detection and characterization of quantitative trait loci for coleoptile elongation under anaerobic conditions in rice |
| topic | anaerobic germination coleoptile elongation direct-seeded rice flooding stress oxyrase quantitative trait locus |
| url | http://dx.doi.org/10.1080/1343943X.2020.1740600 |
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