CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA
Background: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose...
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| Format: | Article |
| Language: | English |
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Mattioli1885
2021-04-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mjhid.org/3214/index.php/mjhid/article/view/4580 |
| _version_ | 1819281756149252096 |
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| author | Florence Urio Matilda Mkombachepa Gration Rwegasira Twilumba Makene Billy Ngasala Teddy Mselle Julie Makani Lucio Luzzatto |
| author_facet | Florence Urio Matilda Mkombachepa Gration Rwegasira Twilumba Makene Billy Ngasala Teddy Mselle Julie Makani Lucio Luzzatto |
| author_sort | Florence Urio |
| collection | DOAJ |
| description | Background: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose, as well as for investigating drug resistance and possible new therapies. Here we report that the Trager & Jensen continuous culture of P. falciparum can be established in a laboratory in Tanzania with minimal facilities and with modest expenditure.
Methodology: This was an in-vitro set up of continuous culture of Plasmodium falciparum study, carried out in 2016-2020 at Muhimbili university of health and allied sciences, Dar-es salaam. Parasite samples were obtained from patients with acute malaria, frozen parasites, and live cultures. Data was collected and analyzed using GraphPad Prism version 8.
Results: We have successfully achieved exponential growth of existing strains that are used worldwide, as well as of parasites in clinical samples from patients with acute malaria. In the aim to optimize growth we have compared human serum and bovine serum albumin as components of the culture media. Additionally, culture synchronization has been achieved using sorbitol.
Conclusion: This experimental system is now available to our institution and to researchers aiming at investigating drug sensitivity and mechanisms of protection against Plasmodium falciparum that accrue from various genes expressed in red cells. |
| first_indexed | 2024-12-24T01:04:45Z |
| format | Article |
| id | doaj.art-52cdc827146647c89bc685482c2b38a3 |
| institution | Directory Open Access Journal |
| issn | 2035-3006 |
| language | English |
| last_indexed | 2024-12-24T01:04:45Z |
| publishDate | 2021-04-01 |
| publisher | Mattioli1885 |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj.art-52cdc827146647c89bc685482c2b38a32022-12-21T17:23:14ZengMattioli1885Mediterranean Journal of Hematology and Infectious Diseases2035-30062021-04-01131CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIAFlorence Urio0Matilda MkombachepaGration RwegasiraTwilumba MakeneBilly NgasalaTeddy MselleJulie MakaniLucio LuzzattoMuhimbili University of Health and Allied SciencesBackground: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose, as well as for investigating drug resistance and possible new therapies. Here we report that the Trager & Jensen continuous culture of P. falciparum can be established in a laboratory in Tanzania with minimal facilities and with modest expenditure. Methodology: This was an in-vitro set up of continuous culture of Plasmodium falciparum study, carried out in 2016-2020 at Muhimbili university of health and allied sciences, Dar-es salaam. Parasite samples were obtained from patients with acute malaria, frozen parasites, and live cultures. Data was collected and analyzed using GraphPad Prism version 8. Results: We have successfully achieved exponential growth of existing strains that are used worldwide, as well as of parasites in clinical samples from patients with acute malaria. In the aim to optimize growth we have compared human serum and bovine serum albumin as components of the culture media. Additionally, culture synchronization has been achieved using sorbitol. Conclusion: This experimental system is now available to our institution and to researchers aiming at investigating drug sensitivity and mechanisms of protection against Plasmodium falciparum that accrue from various genes expressed in red cells.https://mjhid.org/3214/index.php/mjhid/article/view/4580Plasmodium falciparumin vitro culturesAlbumax IIHuman sera |
| spellingShingle | Florence Urio Matilda Mkombachepa Gration Rwegasira Twilumba Makene Billy Ngasala Teddy Mselle Julie Makani Lucio Luzzatto CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA Mediterranean Journal of Hematology and Infectious Diseases Plasmodium falciparum in vitro cultures Albumax II Human sera |
| title | CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA |
| title_full | CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA |
| title_fullStr | CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA |
| title_full_unstemmed | CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA |
| title_short | CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA |
| title_sort | continuous cultures of plasmodium falciparum established in tanzania from patients with acute malaria |
| topic | Plasmodium falciparum in vitro cultures Albumax II Human sera |
| url | https://mjhid.org/3214/index.php/mjhid/article/view/4580 |
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