Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B
Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, <i>Planococcus</i&...
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| author | Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit |
| author_facet | Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit |
| author_sort | Michal Styczynski |
| collection | DOAJ |
| description | Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B. The physiological analysis of these strains revealed their potential to produce various biotechnologically valuable secondary metabolites, including surfactants, siderophores, and orange pigments. The genomic characterization of ANT_H30 and ANT_H53B allowed the identification of genes responsible for the production of carotenoids and the in silico reconstruction of the pigment biosynthesis pathways. The complex manual annotation of the bacterial genomes revealed the metabolic potential to degrade a wide variety of compounds, including xenobiotics and waste materials. Carotenoids produced by these bacteria were analyzed chromatographically, and we proved their activity as scavengers of free radicals. The quantity of crude carotenoid extracts produced at two temperatures using various media was also determined. This was a step toward the optimization of carotenoid production by Antarctic bacteria on a larger scale. |
| first_indexed | 2024-03-10T16:07:30Z |
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| issn | 1420-3049 |
| language | English |
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| publishDate | 2020-09-01 |
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| series | Molecules |
| spelling | doaj.art-52cfe404ee444a9eb9a3f0e2d3cf3da32023-11-20T14:44:25ZengMDPI AGMolecules1420-30492020-09-012519435710.3390/molecules25194357Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53BMichal Styczynski0Agata Rogowska1Katarzyna Gieczewska2Maciej Garstka3Anna Szakiel4Lukasz Dziewit5Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Plant Anatomy and Cytology, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Plant Biochemistry, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandAntarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B. The physiological analysis of these strains revealed their potential to produce various biotechnologically valuable secondary metabolites, including surfactants, siderophores, and orange pigments. The genomic characterization of ANT_H30 and ANT_H53B allowed the identification of genes responsible for the production of carotenoids and the in silico reconstruction of the pigment biosynthesis pathways. The complex manual annotation of the bacterial genomes revealed the metabolic potential to degrade a wide variety of compounds, including xenobiotics and waste materials. Carotenoids produced by these bacteria were analyzed chromatographically, and we proved their activity as scavengers of free radicals. The quantity of crude carotenoid extracts produced at two temperatures using various media was also determined. This was a step toward the optimization of carotenoid production by Antarctic bacteria on a larger scale.https://www.mdpi.com/1420-3049/25/19/4357antarcticacarotenoid<i>Planococcus</i><i>Rhodococcus</i>secondary metabolite |
| spellingShingle | Michal Styczynski Agata Rogowska Katarzyna Gieczewska Maciej Garstka Anna Szakiel Lukasz Dziewit Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B Molecules antarctica carotenoid <i>Planococcus</i> <i>Rhodococcus</i> secondary metabolite |
| title | Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B |
| title_full | Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B |
| title_fullStr | Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B |
| title_full_unstemmed | Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B |
| title_short | Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, <i>Planococcus</i> sp. ANT_H30 and <i>Rhodococcus</i> sp. ANT_H53B |
| title_sort | genome based insights into the production of carotenoids by antarctic bacteria i planococcus i sp ant h30 and i rhodococcus i sp ant h53b |
| topic | antarctica carotenoid <i>Planococcus</i> <i>Rhodococcus</i> secondary metabolite |
| url | https://www.mdpi.com/1420-3049/25/19/4357 |
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