TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound
Due to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea of this research was to use retrorsine as a surrogate reference compound instead of lycopsamine reference or lycopsamine isolated from comfrey. A method for the analysis of lycop...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2014-12-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2014-0031 |
| _version_ | 1819020119225925632 |
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| author | Janeš Damjan Kreft Samo |
| author_facet | Janeš Damjan Kreft Samo |
| author_sort | Janeš Damjan |
| collection | DOAJ |
| description | Due to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea of this research was to use retrorsine as a surrogate reference compound instead of lycopsamine reference or lycopsamine isolated from comfrey. A method for the analysis of lycopsamine in extracts of comfrey roots was developed and validated, employing thin layer chromatography, derivatisation with Dann-Mattocks reagent followed by densitometric analysis. The new method showed linearity within 0.70 to 7.0 μg of lycopsamine per application of 10 μL of a solution. It has also been proven to be specific and precise (repeatability RSD 2-4 % within the plate). The method was successfully employed for quantification of lycopsamine in comfrey root and comfrey root medicinal products such as ointments. |
| first_indexed | 2024-12-21T03:46:08Z |
| format | Article |
| id | doaj.art-52d501228708474b9a6bfa441ada295a |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-21T03:46:08Z |
| publishDate | 2014-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-52d501228708474b9a6bfa441ada295a2022-12-21T19:17:05ZengSciendoActa Pharmaceutica1846-95582014-12-0164450350810.2478/acph-2014-0031acph-2014-0031TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compoundJaneš Damjan0Kreft Samo1Faculty of Pharmacy University of Ljubljana SI-1000 Ljubljana, SloveniaFaculty of Pharmacy University of Ljubljana SI-1000 Ljubljana, SloveniaDue to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea of this research was to use retrorsine as a surrogate reference compound instead of lycopsamine reference or lycopsamine isolated from comfrey. A method for the analysis of lycopsamine in extracts of comfrey roots was developed and validated, employing thin layer chromatography, derivatisation with Dann-Mattocks reagent followed by densitometric analysis. The new method showed linearity within 0.70 to 7.0 μg of lycopsamine per application of 10 μL of a solution. It has also been proven to be specific and precise (repeatability RSD 2-4 % within the plate). The method was successfully employed for quantification of lycopsamine in comfrey root and comfrey root medicinal products such as ointments.https://doi.org/10.2478/acph-2014-0031comfreylycopsamineretrorsinetlcdensitometry |
| spellingShingle | Janeš Damjan Kreft Samo TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound Acta Pharmaceutica comfrey lycopsamine retrorsine tlc densitometry |
| title | TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound |
| title_full | TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound |
| title_fullStr | TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound |
| title_full_unstemmed | TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound |
| title_short | TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound |
| title_sort | tlc densitometric method for screening of lycopsamine in comfrey root symphytum officinale l extracts using retrorsine as a reference compound |
| topic | comfrey lycopsamine retrorsine tlc densitometry |
| url | https://doi.org/10.2478/acph-2014-0031 |
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