Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies
The effects of an alpha-ketoglutarate-supplemented diet on lifespan and functional senescence were evaluated in the Canton S strain of Drosophila melanogaster. The results suggest that effects of dietary alpha-ketoglutarate (AKG) are dose- and gender-dependent. In males, diets containing 1-10 mM AKG...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-11-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2018/11/Lylyk_6.pdf |
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| author | M. P. Lylyk M. M. Bayliak H. V. Shmihel J. M. Storey K. B. Storey V. I. Lushchak |
| author_facet | M. P. Lylyk M. M. Bayliak H. V. Shmihel J. M. Storey K. B. Storey V. I. Lushchak |
| author_sort | M. P. Lylyk |
| collection | DOAJ |
| description | The effects of an alpha-ketoglutarate-supplemented diet on lifespan and functional senescence were evaluated in the Canton S strain of Drosophila melanogaster. The results suggest that effects of dietary alpha-ketoglutarate (AKG) are dose- and gender-dependent. In males, diets containing 1-10 mM AKG did not affect mean and maximum lifespans, except that an increased maximum lifespan observed at 10 mM AKG. Diet with 20 mM AKG shortened median lifespan and had no effect on maximum lifespan of males. In females, diets with low concentrations of AKG (1 and 5 mM) did not affect lifespan, whereas diets supplemented with 10 and 20 mM AKG increased both median and maximum lifespans. At a lifespan-prolonging concentration (10 mM), AKG decreased fecundity, increased cold resistance and had no effect on climbing activity or resistance to oxidative stress in flies of either gender at middle (24 days) and old (40 days) ages. Moreover, middle-aged AKG-fed females but not males were more resistant to heat stress that was accompanied by higher levels of HSP90 protein as compared with controls. Middle-aged flies on AKG-supplemented diets showed elevated oxidative stress and had higher total protein and triacylglycerol levels as compared with controls. Hence, anti-aging effects of AKG do not seem to be related to preventing oxidative stress development but involve metabolic rearrangement and synthesis of specific protective proteins, which aid to resist destructive processes with age. |
| first_indexed | 2024-03-09T08:56:04Z |
| format | Article |
| id | doaj.art-52d7f492ff7f40799cae095925623574 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T08:56:04Z |
| publishDate | 2018-11-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-52d7f492ff7f40799cae0959256235742023-12-02T12:56:57ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032018-11-01906496110.15407/ubj90.06.049Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster fliesM. P. Lylyk0M. M. Bayliak1H. V. Shmihel2J. M. Storey3K. B. Storey4V. I. Lushchak5Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineVasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineVasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineInstitute of Biochemistry, Carleton University, Ottawa, CanadaInstitute of Biochemistry, Carleton University, Ottawa, CanadaVasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, UkraineThe effects of an alpha-ketoglutarate-supplemented diet on lifespan and functional senescence were evaluated in the Canton S strain of Drosophila melanogaster. The results suggest that effects of dietary alpha-ketoglutarate (AKG) are dose- and gender-dependent. In males, diets containing 1-10 mM AKG did not affect mean and maximum lifespans, except that an increased maximum lifespan observed at 10 mM AKG. Diet with 20 mM AKG shortened median lifespan and had no effect on maximum lifespan of males. In females, diets with low concentrations of AKG (1 and 5 mM) did not affect lifespan, whereas diets supplemented with 10 and 20 mM AKG increased both median and maximum lifespans. At a lifespan-prolonging concentration (10 mM), AKG decreased fecundity, increased cold resistance and had no effect on climbing activity or resistance to oxidative stress in flies of either gender at middle (24 days) and old (40 days) ages. Moreover, middle-aged AKG-fed females but not males were more resistant to heat stress that was accompanied by higher levels of HSP90 protein as compared with controls. Middle-aged flies on AKG-supplemented diets showed elevated oxidative stress and had higher total protein and triacylglycerol levels as compared with controls. Hence, anti-aging effects of AKG do not seem to be related to preventing oxidative stress development but involve metabolic rearrangement and synthesis of specific protective proteins, which aid to resist destructive processes with age.http://ukrbiochemjournal.org/wp-content/uploads/2018/11/Lylyk_6.pdfclimbing activityfecundityoxidative stressstress resistanceTCA cycle |
| spellingShingle | M. P. Lylyk M. M. Bayliak H. V. Shmihel J. M. Storey K. B. Storey V. I. Lushchak Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies The Ukrainian Biochemical Journal climbing activity fecundity oxidative stress stress resistance TCA cycle |
| title | Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies |
| title_full | Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies |
| title_fullStr | Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies |
| title_full_unstemmed | Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies |
| title_short | Effects of alpha-ketoglutarate on lifespan and functional aging of Drosophila melanogaster flies |
| title_sort | effects of alpha ketoglutarate on lifespan and functional aging of drosophila melanogaster flies |
| topic | climbing activity fecundity oxidative stress stress resistance TCA cycle |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2018/11/Lylyk_6.pdf |
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