Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia
Background: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2018-12-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | https://doi.org/10.18502/rmm.v6i2.4537 |
| _version_ | 1819203021985284096 |
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| author | Behnaz Nateghi Parisa Behshood Sima Fathullahzadeh Omid Mardanshah |
| author_facet | Behnaz Nateghi Parisa Behshood Sima Fathullahzadeh Omid Mardanshah |
| author_sort | Behnaz Nateghi |
| collection | DOAJ |
| description | Background: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding and regulatory RNAs.The present study was conducted to investigate the association of miR-95 expression with CLL by quantitative real-time PCR.Materials and Methods: Sixty samples, including 30 CLL and 30 healthy controls, were sampled during a period of 4 months. The expression of miR-95 was evaluated by quantitative real-time PCR in peripheral blood mononuclear cells from patients with CLL and in healthy subjects. Additionally, in silico pathway enrichment analysis was performed on validated and predicted targets of miR-95 in several databases, including miRecords and miRTarBase, while the interactions between predicted putative lncRNAs and genes and miRNA expression were examined with miRWalk.Results: The expression of miR-95 was found to be significantly reduced in patients with CLL compared to that in healthy controls (P < 0.005).Conclusion: miR-95 showed potential as a biomarker for the early diagnosis of patients with CLL. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes and may be important regulators in tumorigenesis. |
| first_indexed | 2024-12-23T04:13:18Z |
| format | Article |
| id | doaj.art-52dca43a56e04af281ca26c78797be32 |
| institution | Directory Open Access Journal |
| issn | 2322-1348 |
| language | English |
| last_indexed | 2024-12-23T04:13:18Z |
| publishDate | 2018-12-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-52dca43a56e04af281ca26c78797be322022-12-21T18:00:25ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482018-12-0120171810.18502/rmm.v6i2.4537rmm.v6i2.4537Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic LeukemiaBehnaz Nateghi0Parisa Behshood1Sima Fathullahzadeh2Omid Mardanshah3 Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, Iran Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, Iran Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, Iran Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, IranBackground: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding and regulatory RNAs.The present study was conducted to investigate the association of miR-95 expression with CLL by quantitative real-time PCR.Materials and Methods: Sixty samples, including 30 CLL and 30 healthy controls, were sampled during a period of 4 months. The expression of miR-95 was evaluated by quantitative real-time PCR in peripheral blood mononuclear cells from patients with CLL and in healthy subjects. Additionally, in silico pathway enrichment analysis was performed on validated and predicted targets of miR-95 in several databases, including miRecords and miRTarBase, while the interactions between predicted putative lncRNAs and genes and miRNA expression were examined with miRWalk.Results: The expression of miR-95 was found to be significantly reduced in patients with CLL compared to that in healthy controls (P < 0.005).Conclusion: miR-95 showed potential as a biomarker for the early diagnosis of patients with CLL. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes and may be important regulators in tumorigenesis.https://doi.org/10.18502/rmm.v6i2.4537chronic lymphocytic leukemia, long non-coding RNAs, miR-95, miRNA |
| spellingShingle | Behnaz Nateghi Parisa Behshood Sima Fathullahzadeh Omid Mardanshah Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia Research in Molecular Medicine chronic lymphocytic leukemia, long non-coding RNAs, miR-95, miRNA |
| title | Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia |
| title_full | Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia |
| title_fullStr | Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia |
| title_full_unstemmed | Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia |
| title_short | Circulating miR-95 Is a Potential Biomarker of Chronic Lymphocytic Leukemia |
| title_sort | circulating mir 95 is a potential biomarker of chronic lymphocytic leukemia |
| topic | chronic lymphocytic leukemia, long non-coding RNAs, miR-95, miRNA |
| url | https://doi.org/10.18502/rmm.v6i2.4537 |
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