Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells
Background: Despite numerous studies over a decade, it still remains controversial about the biological effects of RF EMF emitted by mobile phone telephony. Objective: Here we investigated the effect of 900 MHz GSM on the induction of oxidative stress and the level of intracellular reactive oxyge...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2015-09-01
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| Series: | Journal of Biomedical Physics and Engineering |
| Subjects: | |
| Online Access: | http://www.jbpe.org/Journal_OJS/JBPE/index.php/jbpe/article/view/402 |
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| author | Mortazavi S.M.J. Ali-Ghanbari A. Sharifzadeh S. Ranjbaran R. Mostafavi-pour Z. Zal F. Haghani M. |
| author_facet | Mortazavi S.M.J. Ali-Ghanbari A. Sharifzadeh S. Ranjbaran R. Mostafavi-pour Z. Zal F. Haghani M. |
| author_sort | Mortazavi S.M.J. |
| collection | DOAJ |
| description | Background: Despite numerous studies over a decade, it still remains controversial
about the biological effects of RF EMF emitted by mobile phone telephony.
Objective: Here we investigated the effect of 900 MHz GSM on the induction of
oxidative stress and the level of intracellular reactive oxygen species (ROS) in human
mononuclear cells, monocytes and lymphocytes as defence system cells.
Method: 6 ml Peripheral Blood samples were obtained from 13 healthy volunteers
(21-30 year-old). Each sample was devided into 2 groups: one was exposed RF radiation
emitted from a mobile phone simulator for 2 hour and the other used as control
group which was not exposed to any fields. After that, mononuclear cells were isolated
from peripheral blood by density gradient centrifugation in Ficoll-Paque. The intracellular
ROS content in monocytes and lymphocytes was measured by the CM-H2DCFDA
fluorescence probe using flowcytometry technique.
Results: Our results showed significant increase in ROS production after exposure
in population rich in monocytes. This effect was not significant in population rich in
lymphocytes in comparison with non exposed cells.
Conclusion: The results obtained in this study clearly showed the oxidative stress
induction capability of RF electromagnetic field in the portion of PBMCs mostly in
monocytes, like the case of exposure to micro organisms, although the advantages or
disadvantages of this effect should be evaluated. |
| first_indexed | 2024-12-24T23:03:39Z |
| format | Article |
| id | doaj.art-52dd3d4830fa4a25aca5f9fa8a4bed62 |
| institution | Directory Open Access Journal |
| issn | 2251-7200 2251-7200 |
| language | English |
| last_indexed | 2024-12-24T23:03:39Z |
| publishDate | 2015-09-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Biomedical Physics and Engineering |
| spelling | doaj.art-52dd3d4830fa4a25aca5f9fa8a4bed622022-12-21T16:35:04ZengShiraz University of Medical SciencesJournal of Biomedical Physics and Engineering2251-72002251-72002015-09-0153105114Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells Mortazavi S.M.J.0Ali-Ghanbari A.1Sharifzadeh S.2 Ranjbaran R.3 Mostafavi-pour Z.4 Zal F.5Haghani M.6Medical Physics Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | The Center for Research on Protection against Ionizing and Non-ionizing Radiation, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranTelecommunication Department, School of Engineering, Shiraz University, Shiraz, IranDiagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranDiagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranThe Center for Research on Protection against Ionizing and Non-ionizing Radiation, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranBackground: Despite numerous studies over a decade, it still remains controversial about the biological effects of RF EMF emitted by mobile phone telephony. Objective: Here we investigated the effect of 900 MHz GSM on the induction of oxidative stress and the level of intracellular reactive oxygen species (ROS) in human mononuclear cells, monocytes and lymphocytes as defence system cells. Method: 6 ml Peripheral Blood samples were obtained from 13 healthy volunteers (21-30 year-old). Each sample was devided into 2 groups: one was exposed RF radiation emitted from a mobile phone simulator for 2 hour and the other used as control group which was not exposed to any fields. After that, mononuclear cells were isolated from peripheral blood by density gradient centrifugation in Ficoll-Paque. The intracellular ROS content in monocytes and lymphocytes was measured by the CM-H2DCFDA fluorescence probe using flowcytometry technique. Results: Our results showed significant increase in ROS production after exposure in population rich in monocytes. This effect was not significant in population rich in lymphocytes in comparison with non exposed cells. Conclusion: The results obtained in this study clearly showed the oxidative stress induction capability of RF electromagnetic field in the portion of PBMCs mostly in monocytes, like the case of exposure to micro organisms, although the advantages or disadvantages of this effect should be evaluated.http://www.jbpe.org/Journal_OJS/JBPE/index.php/jbpe/article/view/402Electromagnetic RadiationRadiofrequency (RF)Mobile phoneReactive Oxygen Species (ROS) |
| spellingShingle | Mortazavi S.M.J. Ali-Ghanbari A. Sharifzadeh S. Ranjbaran R. Mostafavi-pour Z. Zal F. Haghani M. Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells Journal of Biomedical Physics and Engineering Electromagnetic Radiation Radiofrequency (RF) Mobile phone Reactive Oxygen Species (ROS) |
| title | Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells |
| title_full | Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells |
| title_fullStr | Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells |
| title_full_unstemmed | Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells |
| title_short | Effect of 900 MHz Electromagnetic Radiation on the Induction of ROS in Human Peripheral Blood Mononuclear Cells |
| title_sort | effect of 900 mhz electromagnetic radiation on the induction of ros in human peripheral blood mononuclear cells |
| topic | Electromagnetic Radiation Radiofrequency (RF) Mobile phone Reactive Oxygen Species (ROS) |
| url | http://www.jbpe.org/Journal_OJS/JBPE/index.php/jbpe/article/view/402 |
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