Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens
In this study, a novel antimicrobial formula that incorporates Listeria bacteriophage P100 and silver nanoparticles into an alginate matrix was successfully developed. Paper coated with the antimicrobial formula inhibited the growth of Listeria monocytogenes. The effects of alginate concentration on...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2478 |
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| author | Thanh Tung Lai Thi Thanh Ha Pham Marijn van Lingen Gabrielle Desaulniers Guy Njamen Balázs Tolnai Tarik Jabrane Sylvain Moineau Simon Barnabé |
| author_facet | Thanh Tung Lai Thi Thanh Ha Pham Marijn van Lingen Gabrielle Desaulniers Guy Njamen Balázs Tolnai Tarik Jabrane Sylvain Moineau Simon Barnabé |
| author_sort | Thanh Tung Lai |
| collection | DOAJ |
| description | In this study, a novel antimicrobial formula that incorporates Listeria bacteriophage P100 and silver nanoparticles into an alginate matrix was successfully developed. Paper coated with the antimicrobial formula inhibited the growth of Listeria monocytogenes. The effects of alginate concentration on the formation of silver nanoparticles, silver concentration on the infectivity of phages, and of low alginate concentrations on the sustained release of silver and phages were explored. The highest antimicrobial activity of the alginate–silver coating was achieved with an alginate concentration of 1%. Adding phage P100 (10<sup>9</sup> PFU/mL) into the alginate–silver coating led to a synergic effect that resulted in a 5-log reduction in <i>L. monocytogenes</i>. A bioactive paper was then developed by coating a base paper with the antimicrobial formula at different coating weights, followed by infrared drying. The higher coating weight was a crucial factor for the maintenance of phage infectivity throughout the coating and drying processes. Phages incorporated into the alginate matrix remained functional even after high-temperature infrared drying. Taken together, an optimized coating matrix is critical in improving the antimicrobial performance of bioactive paper as well as maintaining phage infectivity during the paper manufacturing process. |
| first_indexed | 2024-03-09T18:34:05Z |
| format | Article |
| id | doaj.art-52e034b36bab485e8428e3c418db599e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T18:34:05Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-52e034b36bab485e8428e3c418db599e2023-11-24T07:17:52ZengMDPI AGViruses1999-49152022-11-011411247810.3390/v14112478Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne PathogensThanh Tung Lai0Thi Thanh Ha Pham1Marijn van Lingen2Gabrielle Desaulniers3Guy Njamen4Balázs Tolnai5Tarik Jabrane6Sylvain Moineau7Simon Barnabé8Institut d’Innovations Écomatériaux, Écoproduits et Écoénergies à Base de Biomasse, Université du Québec à Trois-Rivières (I2E3, UQTR), Trois-Rivières, QC G8Z 4M3, CanadaInstitut d’Innovations Écomatériaux, Écoproduits et Écoénergies à Base de Biomasse, Université du Québec à Trois-Rivières (I2E3, UQTR), Trois-Rivières, QC G8Z 4M3, CanadaInstitut d’Innovations Écomatériaux, Écoproduits et Écoénergies à Base de Biomasse, Université du Québec à Trois-Rivières (I2E3, UQTR), Trois-Rivières, QC G8Z 4M3, CanadaInstitut d’Innovations Écomatériaux, Écoproduits et Écoénergies à Base de Biomasse, Université du Québec à Trois-Rivières (I2E3, UQTR), Trois-Rivières, QC G8Z 4M3, CanadaKruger Inc., Montréal, QC H3S 1G5, CanadaKruger Inc., Montréal, QC H3S 1G5, CanadaInnofibre—Centre d’Innovation des Produits Cellulosiques, Cégep de Trois-Rivières, Trois-Rivières, QC G9A 5E6, CanadaDépartement de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Québec, QC G1V 0A6, CanadaInstitut d’Innovations Écomatériaux, Écoproduits et Écoénergies à Base de Biomasse, Université du Québec à Trois-Rivières (I2E3, UQTR), Trois-Rivières, QC G8Z 4M3, CanadaIn this study, a novel antimicrobial formula that incorporates Listeria bacteriophage P100 and silver nanoparticles into an alginate matrix was successfully developed. Paper coated with the antimicrobial formula inhibited the growth of Listeria monocytogenes. The effects of alginate concentration on the formation of silver nanoparticles, silver concentration on the infectivity of phages, and of low alginate concentrations on the sustained release of silver and phages were explored. The highest antimicrobial activity of the alginate–silver coating was achieved with an alginate concentration of 1%. Adding phage P100 (10<sup>9</sup> PFU/mL) into the alginate–silver coating led to a synergic effect that resulted in a 5-log reduction in <i>L. monocytogenes</i>. A bioactive paper was then developed by coating a base paper with the antimicrobial formula at different coating weights, followed by infrared drying. The higher coating weight was a crucial factor for the maintenance of phage infectivity throughout the coating and drying processes. Phages incorporated into the alginate matrix remained functional even after high-temperature infrared drying. Taken together, an optimized coating matrix is critical in improving the antimicrobial performance of bioactive paper as well as maintaining phage infectivity during the paper manufacturing process.https://www.mdpi.com/1999-4915/14/11/2478antimicrobial activitybacteriophages<i>Listeria monocytogenes</i>paper coatingsilver nanoparticles |
| spellingShingle | Thanh Tung Lai Thi Thanh Ha Pham Marijn van Lingen Gabrielle Desaulniers Guy Njamen Balázs Tolnai Tarik Jabrane Sylvain Moineau Simon Barnabé Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens Viruses antimicrobial activity bacteriophages <i>Listeria monocytogenes</i> paper coating silver nanoparticles |
| title | Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens |
| title_full | Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens |
| title_fullStr | Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens |
| title_full_unstemmed | Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens |
| title_short | Development of Antimicrobial Paper Coatings Containing Bacteriophages and Silver Nanoparticles for Control of Foodborne Pathogens |
| title_sort | development of antimicrobial paper coatings containing bacteriophages and silver nanoparticles for control of foodborne pathogens |
| topic | antimicrobial activity bacteriophages <i>Listeria monocytogenes</i> paper coating silver nanoparticles |
| url | https://www.mdpi.com/1999-4915/14/11/2478 |
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