Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model
Gastroesophageal reflux disease (GERD) has been growing globally, with an increasing burden on the healthcare system due to multiple factors, such as aging and obesity. The current study evaluated the feasibility of endoscopic balloon-assisted laser treatment (EBLT) in a porcine model. GERD was init...
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MDPI AG
2023-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1656 |
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| author | Boram Cha Hyejin Kim Van Gia Truong Sun-Ju Oh Seok Jeong Hyun Wook Kang |
| author_facet | Boram Cha Hyejin Kim Van Gia Truong Sun-Ju Oh Seok Jeong Hyun Wook Kang |
| author_sort | Boram Cha |
| collection | DOAJ |
| description | Gastroesophageal reflux disease (GERD) has been growing globally, with an increasing burden on the healthcare system due to multiple factors, such as aging and obesity. The current study evaluated the feasibility of endoscopic balloon-assisted laser treatment (EBLT) in a porcine model. GERD was initially developed in three animals via botulinum toxin injection into lower esophageal sphincter (LES). A week after the injection, the EBLT was performed on the GERD-developed models (control = 1 vs. treated = 2). A dose of 30 W of 980 nm laser light was endoscopically applied for 90 s to the LES. Both endoscopic ultrasound and manometry were performed before and after the EBLT. After 12 weeks, esophageal tissues were extracted and prepared for histological analysis. The maximum mucosa temperature was below 50 °C during the EBLT. Compared to control, the treated group yielded thicker and shorter LES muscle layers and maintained LES pressure. Through histology, the EBLT reinforced the muscularis layer with preserved mucosa and mild remodeling of the intermuscular collagen in the LES. The current study demonstrated the feasibility of EBLT as a new endoscopic approach for GERD. Further studies will examine the EBLT in a larger number of animals to warrant efficacy and safety for clinical translations |
| first_indexed | 2024-03-11T02:44:39Z |
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| id | doaj.art-52e0f61121d44c14b889b7a3cccb2afa |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:44:39Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-52e0f61121d44c14b889b7a3cccb2afa2023-11-18T09:26:22ZengMDPI AGBiomedicines2227-90592023-06-01116165610.3390/biomedicines11061656Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine ModelBoram Cha0Hyejin Kim1Van Gia Truong2Sun-Ju Oh3Seok Jeong4Hyun Wook Kang5Department of Internal Medicine, Inha University Hospital, Inha University College of Medicine, Incheon 22332, Republic of KoreaTeCure, Inc., Busan 48548, Republic of KoreaTeCure, Inc., Busan 48548, Republic of KoreaDepartment of Pathology, Kosin University College of Medicine, Busan 49267, Republic of KoreaDepartment of Internal Medicine, Inha University Hospital, Inha University College of Medicine, Incheon 22332, Republic of KoreaTeCure, Inc., Busan 48548, Republic of KoreaGastroesophageal reflux disease (GERD) has been growing globally, with an increasing burden on the healthcare system due to multiple factors, such as aging and obesity. The current study evaluated the feasibility of endoscopic balloon-assisted laser treatment (EBLT) in a porcine model. GERD was initially developed in three animals via botulinum toxin injection into lower esophageal sphincter (LES). A week after the injection, the EBLT was performed on the GERD-developed models (control = 1 vs. treated = 2). A dose of 30 W of 980 nm laser light was endoscopically applied for 90 s to the LES. Both endoscopic ultrasound and manometry were performed before and after the EBLT. After 12 weeks, esophageal tissues were extracted and prepared for histological analysis. The maximum mucosa temperature was below 50 °C during the EBLT. Compared to control, the treated group yielded thicker and shorter LES muscle layers and maintained LES pressure. Through histology, the EBLT reinforced the muscularis layer with preserved mucosa and mild remodeling of the intermuscular collagen in the LES. The current study demonstrated the feasibility of EBLT as a new endoscopic approach for GERD. Further studies will examine the EBLT in a larger number of animals to warrant efficacy and safety for clinical translationshttps://www.mdpi.com/2227-9059/11/6/1656balloon-assisted laser treatmentcatheterendoscopegastroesophageal reflux diseaseporcine model |
| spellingShingle | Boram Cha Hyejin Kim Van Gia Truong Sun-Ju Oh Seok Jeong Hyun Wook Kang Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model Biomedicines balloon-assisted laser treatment catheter endoscope gastroesophageal reflux disease porcine model |
| title | Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model |
| title_full | Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model |
| title_fullStr | Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model |
| title_full_unstemmed | Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model |
| title_short | Feasibility Study on Endoscopic Balloon-Assisted Laser Treatment (EBLT) of Gastroesophageal Reflux Disease (GERD) in In Vivo Porcine Model |
| title_sort | feasibility study on endoscopic balloon assisted laser treatment eblt of gastroesophageal reflux disease gerd in in vivo porcine model |
| topic | balloon-assisted laser treatment catheter endoscope gastroesophageal reflux disease porcine model |
| url | https://www.mdpi.com/2227-9059/11/6/1656 |
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