EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation
Macrophage differentiation is closely associated with idiopathic pulmonary fibrosis (IPF) initiation. Herein, we show that EZY-1, a novel peptide derived from Eucheuma, regulates macrophage differentiation to inhibit IPF. Macrophage differentiation was detected by analyzing the surface antigens of t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623005480 |
| _version_ | 1797356119073488896 |
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| author | Jun Wu Mingjin Tu Ting Wei Xiaoqin Huang Jianming Chen Yufang Jia Liuyan He Xilian Tang Huajun Yu Haitao Zhang |
| author_facet | Jun Wu Mingjin Tu Ting Wei Xiaoqin Huang Jianming Chen Yufang Jia Liuyan He Xilian Tang Huajun Yu Haitao Zhang |
| author_sort | Jun Wu |
| collection | DOAJ |
| description | Macrophage differentiation is closely associated with idiopathic pulmonary fibrosis (IPF) initiation. Herein, we show that EZY-1, a novel peptide derived from Eucheuma, regulates macrophage differentiation to inhibit IPF. Macrophage differentiation was detected by analyzing the surface antigens of the macrophages using flow cytometry. The effect of EZY-1 on cell proliferation was assessed. Signaling molecules involved in macrophage differentiation and fibrosis were detected by Western blot and enzyme-linked immunosorbent assays. The results showed that EZY-1 suppressed BLM-induced pulmonary fibrosis, with a decrease in type M2 macrophages. Simultaneously, EZY-1 reduced the amount of TGF-β1 secreted by M2 macrophages. In addition, EZY-1 downregulated COL1A1, TGF-β, and p-Smad3 and upregulated p-β-catenin in fibroblasts induced by M2 macrophages. Our results confirmed that EZY-1 inhibits IPF by inhibiting type M2 macrophage differentiation, which may be associated with the expression of Rictor and SHP2. Thus, EZY-1 can potentially be used as a drug for treating IPF. |
| first_indexed | 2024-03-08T14:22:07Z |
| format | Article |
| id | doaj.art-52e1d724d00b45d58b4d1dc81c1acbb0 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-08T14:22:07Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-52e1d724d00b45d58b4d1dc81c1acbb02024-01-14T05:37:18ZengElsevierJournal of Functional Foods1756-46462024-01-01112105948EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiationJun Wu0Mingjin Tu1Ting Wei2Xiaoqin Huang3Jianming Chen4Yufang Jia5Liuyan He6Xilian Tang7Huajun Yu8Haitao Zhang9Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaPeptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaPeptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaPeptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524002, ChinaLaboratory Animal Centre, Guangdong Medical University, Zhanjiang 524002, China; Corresponding authors at: Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China (H. Zhang).Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Department of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524002, China; Corresponding authors at: Peptide and Protein Research and Application Key Laboratory of Guangdong Medical University, Zhanjiang, Guangdong 524002, China (H. Zhang).Macrophage differentiation is closely associated with idiopathic pulmonary fibrosis (IPF) initiation. Herein, we show that EZY-1, a novel peptide derived from Eucheuma, regulates macrophage differentiation to inhibit IPF. Macrophage differentiation was detected by analyzing the surface antigens of the macrophages using flow cytometry. The effect of EZY-1 on cell proliferation was assessed. Signaling molecules involved in macrophage differentiation and fibrosis were detected by Western blot and enzyme-linked immunosorbent assays. The results showed that EZY-1 suppressed BLM-induced pulmonary fibrosis, with a decrease in type M2 macrophages. Simultaneously, EZY-1 reduced the amount of TGF-β1 secreted by M2 macrophages. In addition, EZY-1 downregulated COL1A1, TGF-β, and p-Smad3 and upregulated p-β-catenin in fibroblasts induced by M2 macrophages. Our results confirmed that EZY-1 inhibits IPF by inhibiting type M2 macrophage differentiation, which may be associated with the expression of Rictor and SHP2. Thus, EZY-1 can potentially be used as a drug for treating IPF.http://www.sciencedirect.com/science/article/pii/S1756464623005480Idiopathic pulmonary fibrosisEZY-1MacrophagesFibroblasts |
| spellingShingle | Jun Wu Mingjin Tu Ting Wei Xiaoqin Huang Jianming Chen Yufang Jia Liuyan He Xilian Tang Huajun Yu Haitao Zhang EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation Journal of Functional Foods Idiopathic pulmonary fibrosis EZY-1 Macrophages Fibroblasts |
| title | EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation |
| title_full | EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation |
| title_fullStr | EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation |
| title_full_unstemmed | EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation |
| title_short | EZY-1 inhibits idiopathic pulmonary fibrosis by regulating M2-type macrophage differentiation |
| title_sort | ezy 1 inhibits idiopathic pulmonary fibrosis by regulating m2 type macrophage differentiation |
| topic | Idiopathic pulmonary fibrosis EZY-1 Macrophages Fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623005480 |
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