Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract
Although Aloe vera contains numerous bioactive components, the activity principles of widely used A. vera extracts are uncertain. Therefore, we analyzed the effects of genuine A. vera aqueous extract (AV) on human cells with respect to the effects of hydrogen peroxide (H2O2) and 4-hydroxynonenal (HN...
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MDPI AG
2018-09-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/7/10/125 |
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| author | Vera Cesar Iva Jozić Lidija Begović Tea Vuković Selma Mlinarić Hrvoje Lepeduš Suzana Borović Šunjić Neven Žarković |
| author_facet | Vera Cesar Iva Jozić Lidija Begović Tea Vuković Selma Mlinarić Hrvoje Lepeduš Suzana Borović Šunjić Neven Žarković |
| author_sort | Vera Cesar |
| collection | DOAJ |
| description | Although Aloe vera contains numerous bioactive components, the activity principles of widely used A. vera extracts are uncertain. Therefore, we analyzed the effects of genuine A. vera aqueous extract (AV) on human cells with respect to the effects of hydrogen peroxide (H2O2) and 4-hydroxynonenal (HNE). Fully developed A. vera leaves were harvested and analyzed for vitamin C, carotenoids, total soluble phenolic content, and antioxidant capacity. Furthermore, human cervical cancer (HeLa), human microvascular endothelial cells (HMEC), human keratinocytes (HaCat), and human osteosarcoma (HOS) cell cultures were treated with AV extract for one hour after treatment with H2O2 or HNE. The cell number and viability were determined using Trypan Blue, and endogenous reactive oxygen species (ROS) production was determined by fluorescence, while intracellular HNE–protein adducts were measured for the first time ever by genuine cell-based HNE–His ELISA. The AV extract expressed strong antioxidant capacities (1.1 mmol of Trolox eq/g fresh weight) and cell-type-specific influence on the cytotoxicity of H2O2, as well as on endogenous production of ROS and HNE–protein adducts induced by HNE treatment, while AV itself did not induce production of ROS or HNE–protein adducts at all. This study, for the first time, revealed the importance of HNE for the activity principles of AV. Since HMEC cells were the most sensitive to AV, the effects of AV on microvascular endothelia could be of particular importance for the activity principles of Aloe vera extracts. |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:16:05Z |
| publishDate | 2018-09-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-52e8fe3114204eb598e4d356461e396c2023-09-02T22:47:15ZengMDPI AGAntioxidants2076-39212018-09-0171012510.3390/antiox7100125antiox7100125Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera ExtractVera Cesar0Iva Jozić1Lidija Begović2Tea Vuković3Selma Mlinarić4Hrvoje Lepeduš5Suzana Borović Šunjić6Neven Žarković7Department of Biology, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaLaboratory for Oxidative Stress (LabOS), Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, CroatiaDepartment of Biology, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaFaculty of Dental Medicine and Health, Josip Juraj Strossmyer University of Osijek, Cara Hadrijana 10/E, 31000 Osijek, CroatiaLaboratory for Oxidative Stress (LabOS), Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, CroatiaLaboratory for Oxidative Stress (LabOS), Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, CroatiaAlthough Aloe vera contains numerous bioactive components, the activity principles of widely used A. vera extracts are uncertain. Therefore, we analyzed the effects of genuine A. vera aqueous extract (AV) on human cells with respect to the effects of hydrogen peroxide (H2O2) and 4-hydroxynonenal (HNE). Fully developed A. vera leaves were harvested and analyzed for vitamin C, carotenoids, total soluble phenolic content, and antioxidant capacity. Furthermore, human cervical cancer (HeLa), human microvascular endothelial cells (HMEC), human keratinocytes (HaCat), and human osteosarcoma (HOS) cell cultures were treated with AV extract for one hour after treatment with H2O2 or HNE. The cell number and viability were determined using Trypan Blue, and endogenous reactive oxygen species (ROS) production was determined by fluorescence, while intracellular HNE–protein adducts were measured for the first time ever by genuine cell-based HNE–His ELISA. The AV extract expressed strong antioxidant capacities (1.1 mmol of Trolox eq/g fresh weight) and cell-type-specific influence on the cytotoxicity of H2O2, as well as on endogenous production of ROS and HNE–protein adducts induced by HNE treatment, while AV itself did not induce production of ROS or HNE–protein adducts at all. This study, for the first time, revealed the importance of HNE for the activity principles of AV. Since HMEC cells were the most sensitive to AV, the effects of AV on microvascular endothelia could be of particular importance for the activity principles of Aloe vera extracts.http://www.mdpi.com/2076-3921/7/10/125Aloe veraplant extractantioxidantscell growthoxidative stressreactive oxygen species (ROS)hydrogen peroxidelipid peroxidation4-hydroxynonenal (HNE)cell-based ELISAHNE–protein adductsmicrovascular endothelium |
| spellingShingle | Vera Cesar Iva Jozić Lidija Begović Tea Vuković Selma Mlinarić Hrvoje Lepeduš Suzana Borović Šunjić Neven Žarković Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract Antioxidants Aloe vera plant extract antioxidants cell growth oxidative stress reactive oxygen species (ROS) hydrogen peroxide lipid peroxidation 4-hydroxynonenal (HNE) cell-based ELISA HNE–protein adducts microvascular endothelium |
| title | Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract |
| title_full | Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract |
| title_fullStr | Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract |
| title_full_unstemmed | Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract |
| title_short | Cell-Type-Specific Modulation of Hydrogen Peroxide Cytotoxicity and 4-Hydroxynonenal Binding to Human Cellular Proteins In Vitro by Antioxidant Aloe vera Extract |
| title_sort | cell type specific modulation of hydrogen peroxide cytotoxicity and 4 hydroxynonenal binding to human cellular proteins in vitro by antioxidant aloe vera extract |
| topic | Aloe vera plant extract antioxidants cell growth oxidative stress reactive oxygen species (ROS) hydrogen peroxide lipid peroxidation 4-hydroxynonenal (HNE) cell-based ELISA HNE–protein adducts microvascular endothelium |
| url | http://www.mdpi.com/2076-3921/7/10/125 |
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