Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus
BackgroundTobacco mosaic virus (TMV) is one famous plant virus responsible for substantial economic losses worldwide. However, the roles of bacterial communities in response to TMV in the tobacco rhizosphere remain unclear.MethodsWe explored the soil physicochemical properties and bacterial communit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341296/full |
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| author | Yuqiang Zhao Tianbo Liu Shaolong Wu Deyong Zhang Zhipeng Xiao Zuohua Ren Lingling Li Suoni Liu Yunhua Xiao Qianjun Tang |
| author_facet | Yuqiang Zhao Tianbo Liu Shaolong Wu Deyong Zhang Zhipeng Xiao Zuohua Ren Lingling Li Suoni Liu Yunhua Xiao Qianjun Tang |
| author_sort | Yuqiang Zhao |
| collection | DOAJ |
| description | BackgroundTobacco mosaic virus (TMV) is one famous plant virus responsible for substantial economic losses worldwide. However, the roles of bacterial communities in response to TMV in the tobacco rhizosphere remain unclear.MethodsWe explored the soil physicochemical properties and bacterial community succession of the healthy (YTH) and diseased (YTD) plants with TMV infection by 16S rRNA gene sequencing and bioinformatics analysis.ResultsWe found that soil pH in the YTD group was significantly lower than in the YTH group, and the soil available nutrients were substantially higher. The bacterial community analysis found that the diversity and structure significantly differed post-TMV disease onset. With TMV inoculated, the alpha diversity of the bacterial community in the YTD was markedly higher than that in the YTH group at the early stage. However, the alpha diversity in the YTD group subsequently decreased to lower than in the YTH group. The early bacterial structure of healthy plants exhibited higher susceptibility to TMV infection, whereas, in the subsequent stages, there was an enrichment of beneficial bacterial (e.g., Ramlibacter, Sphingomonas, Streptomyces, and Niastella) and enhanced energy metabolism and nucleotide metabolism in bacteria.ConclusionThe initial soil bacterial community exhibited susceptibility to TMV infection, which might contribute to strengthening resistance of Tobacco to TMV. |
| first_indexed | 2024-03-08T09:29:52Z |
| format | Article |
| id | doaj.art-52e990f8ac4547bcb72a3c5df953e7d5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T09:29:52Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-52e990f8ac4547bcb72a3c5df953e7d52024-01-31T04:40:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011510.3389/fmicb.2024.13412961341296Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virusYuqiang Zhao0Tianbo Liu1Shaolong Wu2Deyong Zhang3Zhipeng Xiao4Zuohua Ren5Lingling Li6Suoni Liu7Yunhua Xiao8Qianjun Tang9College of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaHunan Tobacco Company, Changsha, ChinaHunan Tobacco Company, Changsha, ChinaInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, ChinaHunan Tobacco Company, Changsha, ChinaCollege of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaCollege of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaCollege of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaCollege of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaCollege of Plant Protection and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaBackgroundTobacco mosaic virus (TMV) is one famous plant virus responsible for substantial economic losses worldwide. However, the roles of bacterial communities in response to TMV in the tobacco rhizosphere remain unclear.MethodsWe explored the soil physicochemical properties and bacterial community succession of the healthy (YTH) and diseased (YTD) plants with TMV infection by 16S rRNA gene sequencing and bioinformatics analysis.ResultsWe found that soil pH in the YTD group was significantly lower than in the YTH group, and the soil available nutrients were substantially higher. The bacterial community analysis found that the diversity and structure significantly differed post-TMV disease onset. With TMV inoculated, the alpha diversity of the bacterial community in the YTD was markedly higher than that in the YTH group at the early stage. However, the alpha diversity in the YTD group subsequently decreased to lower than in the YTH group. The early bacterial structure of healthy plants exhibited higher susceptibility to TMV infection, whereas, in the subsequent stages, there was an enrichment of beneficial bacterial (e.g., Ramlibacter, Sphingomonas, Streptomyces, and Niastella) and enhanced energy metabolism and nucleotide metabolism in bacteria.ConclusionThe initial soil bacterial community exhibited susceptibility to TMV infection, which might contribute to strengthening resistance of Tobacco to TMV.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341296/fullTobacco mosaic virusbacterial interactionsensitivity of bacterial communitybeneficial bacteriaalpha diversity |
| spellingShingle | Yuqiang Zhao Tianbo Liu Shaolong Wu Deyong Zhang Zhipeng Xiao Zuohua Ren Lingling Li Suoni Liu Yunhua Xiao Qianjun Tang Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus Frontiers in Microbiology Tobacco mosaic virus bacterial interaction sensitivity of bacterial community beneficial bacteria alpha diversity |
| title | Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus |
| title_full | Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus |
| title_fullStr | Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus |
| title_full_unstemmed | Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus |
| title_short | Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus |
| title_sort | insight into the soil bacterial community succession of nicotiana benthamiana in response to tobacco mosaic virus |
| topic | Tobacco mosaic virus bacterial interaction sensitivity of bacterial community beneficial bacteria alpha diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1341296/full |
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