Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines
Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis and orchitis are the most common clinical manifestations in animals. The present study a total of 168 clinical samples were collected from buffaloes (87) and cattle (81...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2017-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-brucella-abortus-from-bovines/ |
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| author | M.D. Shrimali N.M. Shah B.S. Chandel H.C. Chauhan S.S. Patel K.B. Patel B.K. Patel A.G. Bhagat S.I. Patel A.I. Dadawala J.D. Shah Manish Rajgor R.P. Pandya A.C. Patel M.A. Patel J.K. Kala M.G. Patel |
| author_facet | M.D. Shrimali N.M. Shah B.S. Chandel H.C. Chauhan S.S. Patel K.B. Patel B.K. Patel A.G. Bhagat S.I. Patel A.I. Dadawala J.D. Shah Manish Rajgor R.P. Pandya A.C. Patel M.A. Patel J.K. Kala M.G. Patel |
| author_sort | M.D. Shrimali |
| collection | DOAJ |
| description | Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis and orchitis are the most common clinical manifestations in animals. The present study a total of 168 clinical samples were collected from buffaloes (87) and cattle (81). All clinical samples were processed by cultural isolation on Brucella agar medium (BAM) with selective antibiotic supplements and genus specific PCR using B4/B5 (223bp) and F4/R2 (905bp) primer. Out of 168 clinical samples 15 samples yielded Brucella isolates by cultural isolation and 19 samples positive for Brucella organism by genus specific PCR. All genus specific PCR positive 19 samples also positive by Species specific PCR based on omp31, B. abortus +IS711 (498bp) primer. Amplicon of 498bp by B. abortus +IS711 primers indicates that all nineteen samples of cattle and buffaloes were found to be Brucella abortus. |
| first_indexed | 2024-12-20T16:24:59Z |
| format | Article |
| id | doaj.art-52f3b8d89127430bb3dd1e4910209259 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-20T16:24:59Z |
| publishDate | 2017-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-52f3b8d89127430bb3dd1e49102092592022-12-21T19:33:28ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-06-0111293393910.22207/JPAM.11.2.33Isolation, Identification and Molecular Characterization of Brucella abortus from BovinesM.D. Shrimali0N.M. Shah1B.S. Chandel2H.C. Chauhan3S.S. Patel4K.B. Patel5B.K. Patel6A.G. Bhagat7S.I. Patel8A.I. Dadawala9J.D. Shah10Manish Rajgor11R.P. Pandya12A.C. Patel13M.A. Patel14J.K. Kala15M.G. Patel16Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis and orchitis are the most common clinical manifestations in animals. The present study a total of 168 clinical samples were collected from buffaloes (87) and cattle (81). All clinical samples were processed by cultural isolation on Brucella agar medium (BAM) with selective antibiotic supplements and genus specific PCR using B4/B5 (223bp) and F4/R2 (905bp) primer. Out of 168 clinical samples 15 samples yielded Brucella isolates by cultural isolation and 19 samples positive for Brucella organism by genus specific PCR. All genus specific PCR positive 19 samples also positive by Species specific PCR based on omp31, B. abortus +IS711 (498bp) primer. Amplicon of 498bp by B. abortus +IS711 primers indicates that all nineteen samples of cattle and buffaloes were found to be Brucella abortus.https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-brucella-abortus-from-bovines/brucella abortuscultural isolationmolecular characterizationpcr |
| spellingShingle | M.D. Shrimali N.M. Shah B.S. Chandel H.C. Chauhan S.S. Patel K.B. Patel B.K. Patel A.G. Bhagat S.I. Patel A.I. Dadawala J.D. Shah Manish Rajgor R.P. Pandya A.C. Patel M.A. Patel J.K. Kala M.G. Patel Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines Journal of Pure and Applied Microbiology brucella abortus cultural isolation molecular characterization pcr |
| title | Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines |
| title_full | Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines |
| title_fullStr | Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines |
| title_full_unstemmed | Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines |
| title_short | Isolation, Identification and Molecular Characterization of Brucella abortus from Bovines |
| title_sort | isolation identification and molecular characterization of brucella abortus from bovines |
| topic | brucella abortus cultural isolation molecular characterization pcr |
| url | https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-brucella-abortus-from-bovines/ |
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