Dystroglycan is a scaffold for extracellular axon guidance decisions
Axon guidance requires interactions between extracellular signaling molecules and transmembrane receptors, but how appropriate context-dependent decisions are coordinated outside the cell remains unclear. Here we show that the transmembrane glycoprotein Dystroglycan interacts with a changing set of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/42143 |
| _version_ | 1811202709329543168 |
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| author | L Bailey Lindenmaier Nicolas Parmentier Caiying Guo Fadel Tissir Kevin M Wright |
| author_facet | L Bailey Lindenmaier Nicolas Parmentier Caiying Guo Fadel Tissir Kevin M Wright |
| author_sort | L Bailey Lindenmaier |
| collection | DOAJ |
| description | Axon guidance requires interactions between extracellular signaling molecules and transmembrane receptors, but how appropriate context-dependent decisions are coordinated outside the cell remains unclear. Here we show that the transmembrane glycoprotein Dystroglycan interacts with a changing set of environmental cues that regulate the trajectories of extending axons throughout the mammalian brain and spinal cord. Dystroglycan operates primarily as an extracellular scaffold during axon guidance, as it functions non-cell autonomously and does not require signaling through its intracellular domain. We identify the transmembrane receptor Celsr3/Adgrc3 as a binding partner for Dystroglycan, and show that this interaction is critical for specific axon guidance events in vivo. These findings establish Dystroglycan as a multifunctional scaffold that coordinates extracellular matrix proteins, secreted cues, and transmembrane receptors to regulate axon guidance. |
| first_indexed | 2024-04-12T02:42:55Z |
| format | Article |
| id | doaj.art-52f63657a94944cf9ba28737165c2ce4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:42:55Z |
| publishDate | 2019-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-52f63657a94944cf9ba28737165c2ce42022-12-22T03:51:16ZengeLife Sciences Publications LtdeLife2050-084X2019-02-01810.7554/eLife.42143Dystroglycan is a scaffold for extracellular axon guidance decisionsL Bailey Lindenmaier0Nicolas Parmentier1Caiying Guo2Fadel Tissir3Kevin M Wright4https://orcid.org/0000-0001-5094-5270Vollum Institute, Oregon Health & Science University, Portland, United StatesInstitiute of Neuroscience, Université Catholique de Louvain, Brussels, BelgiumJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesInstitiute of Neuroscience, Université Catholique de Louvain, Brussels, BelgiumVollum Institute, Oregon Health & Science University, Portland, United StatesAxon guidance requires interactions between extracellular signaling molecules and transmembrane receptors, but how appropriate context-dependent decisions are coordinated outside the cell remains unclear. Here we show that the transmembrane glycoprotein Dystroglycan interacts with a changing set of environmental cues that regulate the trajectories of extending axons throughout the mammalian brain and spinal cord. Dystroglycan operates primarily as an extracellular scaffold during axon guidance, as it functions non-cell autonomously and does not require signaling through its intracellular domain. We identify the transmembrane receptor Celsr3/Adgrc3 as a binding partner for Dystroglycan, and show that this interaction is critical for specific axon guidance events in vivo. These findings establish Dystroglycan as a multifunctional scaffold that coordinates extracellular matrix proteins, secreted cues, and transmembrane receptors to regulate axon guidance.https://elifesciences.org/articles/42143axon guidancedystroglycancelsr3commissural axoninternal capsuleextracellular matrix |
| spellingShingle | L Bailey Lindenmaier Nicolas Parmentier Caiying Guo Fadel Tissir Kevin M Wright Dystroglycan is a scaffold for extracellular axon guidance decisions eLife axon guidance dystroglycan celsr3 commissural axon internal capsule extracellular matrix |
| title | Dystroglycan is a scaffold for extracellular axon guidance decisions |
| title_full | Dystroglycan is a scaffold for extracellular axon guidance decisions |
| title_fullStr | Dystroglycan is a scaffold for extracellular axon guidance decisions |
| title_full_unstemmed | Dystroglycan is a scaffold for extracellular axon guidance decisions |
| title_short | Dystroglycan is a scaffold for extracellular axon guidance decisions |
| title_sort | dystroglycan is a scaffold for extracellular axon guidance decisions |
| topic | axon guidance dystroglycan celsr3 commissural axon internal capsule extracellular matrix |
| url | https://elifesciences.org/articles/42143 |
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