Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand
Biting midges of the genus <i>Culicoides</i> Latreille are significant pests and vectors that transmit pathogens to humans and other animals. Cattle are among the important livestock that can potentially be severely affected by <i>Culicoides</i>. In this study, we examined th...
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MDPI AG
2023-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/7/574 |
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| author | Bhuvadol Gomontean Kotchaphon Vaisusuk Wasupon Chatan Komgrit Wongpakam Papasara Sankul Laksika Lachanthuek Ronnalit Mintara Isara Thanee Pairot Pramual |
| author_facet | Bhuvadol Gomontean Kotchaphon Vaisusuk Wasupon Chatan Komgrit Wongpakam Papasara Sankul Laksika Lachanthuek Ronnalit Mintara Isara Thanee Pairot Pramual |
| author_sort | Bhuvadol Gomontean |
| collection | DOAJ |
| description | Biting midges of the genus <i>Culicoides</i> Latreille are significant pests and vectors that transmit pathogens to humans and other animals. Cattle are among the important livestock that can potentially be severely affected by <i>Culicoides</i>. In this study, we examined the species diversity, abundance, and host blood meal identification of biting midges in cattle pens located in three different land use types: villages, agricultural areas, and the forest edge. A total of 12,916 biting midges were collected, and most of these were from cattle pens located in villages (34%) and agricultural land (52%). Morphological identification revealed 29 <i>Culicoides</i> species. The most common species were <i>C. oxystoma</i>, <i>C. mahasarakhamense</i>, <i>C. peregrinus</i>, and <i>C. shortti</i>; taken together, these species represented >80% of all specimens collected. Despite midges being less numerous (14% of the total collection), cattle pens located near the forest showed greater diversity (23) than those from villages and agricultural areas. More diverse immature habitats and host blood sources from wildlife in nearby forests possibly explain the greater diversity in the cattle pens near the forest edge. Host blood meal analysis revealed that most (65%) biting midges had fed on buffalo despite the fact that this animal was much less numerous than cows or chickens. Relatively larger size and black-colored skin could be factors that make buffalo more attractive to biting midges than other host species. In this study, we also provided 67 DNA barcoding sequences of 13 species, three of which (<i>C. flaviscutatus</i>, <i>C. geminus</i>, and <i>C. suzukii</i>) were first reported from Thai specimens. DNA barcode analysis indicated cryptic diversity within <i>C. hegneri</i> and <i>C. flavescens</i> in Thailand, and thus, further investigation is required to resolve their species status. |
| first_indexed | 2024-03-11T00:58:37Z |
| format | Article |
| id | doaj.art-52f7321bc3b54b4c978287eb297cb862 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T00:58:37Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-52f7321bc3b54b4c978287eb297cb8622023-11-18T19:48:24ZengMDPI AGInsects2075-44502023-06-0114757410.3390/insects14070574Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from ThailandBhuvadol Gomontean0Kotchaphon Vaisusuk1Wasupon Chatan2Komgrit Wongpakam3Papasara Sankul4Laksika Lachanthuek5Ronnalit Mintara6Isara Thanee7Pairot Pramual8Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Veterinary Technology and Veterinary Nursing, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham 44000, ThailandDepartment of Veterinary Clinic, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, ThailandWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandBiting midges of the genus <i>Culicoides</i> Latreille are significant pests and vectors that transmit pathogens to humans and other animals. Cattle are among the important livestock that can potentially be severely affected by <i>Culicoides</i>. In this study, we examined the species diversity, abundance, and host blood meal identification of biting midges in cattle pens located in three different land use types: villages, agricultural areas, and the forest edge. A total of 12,916 biting midges were collected, and most of these were from cattle pens located in villages (34%) and agricultural land (52%). Morphological identification revealed 29 <i>Culicoides</i> species. The most common species were <i>C. oxystoma</i>, <i>C. mahasarakhamense</i>, <i>C. peregrinus</i>, and <i>C. shortti</i>; taken together, these species represented >80% of all specimens collected. Despite midges being less numerous (14% of the total collection), cattle pens located near the forest showed greater diversity (23) than those from villages and agricultural areas. More diverse immature habitats and host blood sources from wildlife in nearby forests possibly explain the greater diversity in the cattle pens near the forest edge. Host blood meal analysis revealed that most (65%) biting midges had fed on buffalo despite the fact that this animal was much less numerous than cows or chickens. Relatively larger size and black-colored skin could be factors that make buffalo more attractive to biting midges than other host species. In this study, we also provided 67 DNA barcoding sequences of 13 species, three of which (<i>C. flaviscutatus</i>, <i>C. geminus</i>, and <i>C. suzukii</i>) were first reported from Thai specimens. DNA barcode analysis indicated cryptic diversity within <i>C. hegneri</i> and <i>C. flavescens</i> in Thailand, and thus, further investigation is required to resolve their species status.https://www.mdpi.com/2075-4450/14/7/574cattlehost blood mealinsect vectorDNA barcode |
| spellingShingle | Bhuvadol Gomontean Kotchaphon Vaisusuk Wasupon Chatan Komgrit Wongpakam Papasara Sankul Laksika Lachanthuek Ronnalit Mintara Isara Thanee Pairot Pramual Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand Insects cattle host blood meal insect vector DNA barcode |
| title | Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand |
| title_full | Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand |
| title_fullStr | Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand |
| title_full_unstemmed | Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand |
| title_short | Diversity, Abundance and Host Blood Meal Analysis of <i>Culicoides</i> Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand |
| title_sort | diversity abundance and host blood meal analysis of i culicoides i latreille diptera ceratopogonidae from cattle pens in different land use types from thailand |
| topic | cattle host blood meal insect vector DNA barcode |
| url | https://www.mdpi.com/2075-4450/14/7/574 |
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