Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin
<p>Abstract</p> <p>Background</p> <p>Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-11-01
|
| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/8/1/58 |
| _version_ | 1818824628368310272 |
|---|---|
| author | Hashim Onn H Lim Boon-Kiong Abdullah-Soheimi Siti S Shuib Adawiyah S |
| author_facet | Hashim Onn H Lim Boon-Kiong Abdullah-Soheimi Siti S Shuib Adawiyah S |
| author_sort | Hashim Onn H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and screening purposes. In the present study, urine samples from patients with ovarian carcinoma were analysed by two-dimensional gel electrophoresis and the profiles generated were compared to those similarly obtained from age-matched cancer negative women.</p> <p>Results</p> <p>Significant reduced levels of CD59, kininogen-1 and a 39 kDa fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), and enhanced excretion of a 19 kDa fragment of albumin, were detected in the urine of patients with ovarian carcinoma compared to the control subjects. The different altered levels of the proteins were confirmed by Western blotting using antisera and a lectin that bind to the respective proteins.</p> <p>Conclusion</p> <p>CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma.</p> |
| first_indexed | 2024-12-18T23:58:54Z |
| format | Article |
| id | doaj.art-52fb334eda2b4a05bf7337b8a4ac90a6 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-18T23:58:54Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-52fb334eda2b4a05bf7337b8a4ac90a62022-12-21T20:46:35ZengBMCProteome Science1477-59562010-11-01815810.1186/1477-5956-8-58Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albuminHashim Onn HLim Boon-KiongAbdullah-Soheimi Siti SShuib Adawiyah S<p>Abstract</p> <p>Background</p> <p>Diagnosis of ovarian carcinoma is in urgent need for new complementary biomarkers for early stage detection. Proteins that are aberrantly excreted in the urine of cancer patients are excellent biomarker candidates for development of new noninvasive protocol for early diagnosis and screening purposes. In the present study, urine samples from patients with ovarian carcinoma were analysed by two-dimensional gel electrophoresis and the profiles generated were compared to those similarly obtained from age-matched cancer negative women.</p> <p>Results</p> <p>Significant reduced levels of CD59, kininogen-1 and a 39 kDa fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), and enhanced excretion of a 19 kDa fragment of albumin, were detected in the urine of patients with ovarian carcinoma compared to the control subjects. The different altered levels of the proteins were confirmed by Western blotting using antisera and a lectin that bind to the respective proteins.</p> <p>Conclusion</p> <p>CD59, kininogen-1 and fragments of ITIH4 and albumin may be used as complementary biomarkers in the development of new noninvasive protocols for diagnosis and screening of ovarian carcinoma.</p>http://www.proteomesci.com/content/8/1/58 |
| spellingShingle | Hashim Onn H Lim Boon-Kiong Abdullah-Soheimi Siti S Shuib Adawiyah S Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin Proteome Science |
| title | Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin |
| title_full | Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin |
| title_fullStr | Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin |
| title_full_unstemmed | Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin |
| title_short | Patients with ovarian carcinoma excrete different altered levels of urine CD59, kininogen-1 and fragments of inter-alpha-trypsin inhibitor heavy chain H4 and albumin |
| title_sort | patients with ovarian carcinoma excrete different altered levels of urine cd59 kininogen 1 and fragments of inter alpha trypsin inhibitor heavy chain h4 and albumin |
| url | http://www.proteomesci.com/content/8/1/58 |
| work_keys_str_mv | AT hashimonnh patientswithovariancarcinomaexcretedifferentalteredlevelsofurinecd59kininogen1andfragmentsofinteralphatrypsininhibitorheavychainh4andalbumin AT limboonkiong patientswithovariancarcinomaexcretedifferentalteredlevelsofurinecd59kininogen1andfragmentsofinteralphatrypsininhibitorheavychainh4andalbumin AT abdullahsoheimisitis patientswithovariancarcinomaexcretedifferentalteredlevelsofurinecd59kininogen1andfragmentsofinteralphatrypsininhibitorheavychainh4andalbumin AT shuibadawiyahs patientswithovariancarcinomaexcretedifferentalteredlevelsofurinecd59kininogen1andfragmentsofinteralphatrypsininhibitorheavychainh4andalbumin |