Identification of loci associated with conception rate in primiparous Holstein cows
Abstract Background Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each bre...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-6203-2 |
| _version_ | 1811296129297416192 |
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| author | Jennifer N. Kiser Erin Clancey Joao G. N. Moraes Joseph Dalton Gregory W. Burns Thomas E. Spencer Holly L. Neibergs |
| author_facet | Jennifer N. Kiser Erin Clancey Joao G. N. Moraes Joseph Dalton Gregory W. Burns Thomas E. Spencer Holly L. Neibergs |
| author_sort | Jennifer N. Kiser |
| collection | DOAJ |
| description | Abstract Background Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each breeding, are not well characterized. The objectives of this study were to identify loci, positional candidate genes, and transcription factor binding sites (TFBS) associated with CCR and determine if there was a genetic correlation between CCR and milk production in primiparous Holstein cows. Cows were bred via artificial insemination (AI) at either observed estrus or timed AI and pregnancy status was determined at day 35 post-insemination. Additive, dominant, and recessive efficient mixed model association expedited (EMMAX) models were used in two genome-wide association analyses (GWAA). One GWAA focused on CCR at first service (CCR1) comparing cows that conceived and maintained pregnancy to day 35 after the first AI (n = 494) to those that were open after the first AI (n = 538). The second GWAA investigated loci associated with the number of times bred (TBRD) required for conception in cows that either conceived after the first AI (n = 494) or repeated services (n = 472). Results The CCR1 GWAA identified 123, 198, and 76 loci associated (P < 5 × 10− 08) in additive, dominant, and recessive models, respectively. The TBRD GWAA identified 66, 95, and 33 loci associated (P < 5 × 10− 08) in additive, dominant, and recessive models, respectively. Four of the top five loci were shared in CCR1 and TBRD for each GWAA model. Many of the associated loci harbored positional candidate genes and TFBS with putative functional relevance to fertility. Thirty-six of the loci were validated in previous GWAA studies across multiple breeds. None of the CCR1 or TBRD associated loci were associated with milk production, nor was their significance with phenotypic and genetic correlations to 305-day milk production. Conclusions The identification and validation of loci, positional candidate genes, and TFBS associated with CCR1 and TBRD can be utilized to improve, and further characterize the processes involved in cattle fertility. |
| first_indexed | 2024-04-13T05:44:00Z |
| format | Article |
| id | doaj.art-530054cd12064ce4b874fe705b6b8530 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T05:44:00Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-530054cd12064ce4b874fe705b6b85302022-12-22T03:00:00ZengBMCBMC Genomics1471-21642019-11-0120111310.1186/s12864-019-6203-2Identification of loci associated with conception rate in primiparous Holstein cowsJennifer N. Kiser0Erin Clancey1Joao G. N. Moraes2Joseph Dalton3Gregory W. Burns4Thomas E. Spencer5Holly L. Neibergs6Department of Animal Sciences and Center for Reproductive Biology, Washington State UniversityDepartment of Animal Sciences and Center for Reproductive Biology, Washington State UniversityDivision of Animal Sciences, University of MissouriDepartment of Animal and Veterinary Science, University of IdahoDivision of Animal Sciences, University of MissouriDivision of Animal Sciences, University of MissouriDepartment of Animal Sciences and Center for Reproductive Biology, Washington State UniversityAbstract Background Subfertility is a major issue facing the dairy industry as the average US Holstein cow conception rate (CCR) is approximately 35%. The genetics underlying the physiological processes responsible for CCR, the proportion of cows able to conceive and maintain a pregnancy at each breeding, are not well characterized. The objectives of this study were to identify loci, positional candidate genes, and transcription factor binding sites (TFBS) associated with CCR and determine if there was a genetic correlation between CCR and milk production in primiparous Holstein cows. Cows were bred via artificial insemination (AI) at either observed estrus or timed AI and pregnancy status was determined at day 35 post-insemination. Additive, dominant, and recessive efficient mixed model association expedited (EMMAX) models were used in two genome-wide association analyses (GWAA). One GWAA focused on CCR at first service (CCR1) comparing cows that conceived and maintained pregnancy to day 35 after the first AI (n = 494) to those that were open after the first AI (n = 538). The second GWAA investigated loci associated with the number of times bred (TBRD) required for conception in cows that either conceived after the first AI (n = 494) or repeated services (n = 472). Results The CCR1 GWAA identified 123, 198, and 76 loci associated (P < 5 × 10− 08) in additive, dominant, and recessive models, respectively. The TBRD GWAA identified 66, 95, and 33 loci associated (P < 5 × 10− 08) in additive, dominant, and recessive models, respectively. Four of the top five loci were shared in CCR1 and TBRD for each GWAA model. Many of the associated loci harbored positional candidate genes and TFBS with putative functional relevance to fertility. Thirty-six of the loci were validated in previous GWAA studies across multiple breeds. None of the CCR1 or TBRD associated loci were associated with milk production, nor was their significance with phenotypic and genetic correlations to 305-day milk production. Conclusions The identification and validation of loci, positional candidate genes, and TFBS associated with CCR1 and TBRD can be utilized to improve, and further characterize the processes involved in cattle fertility.http://link.springer.com/article/10.1186/s12864-019-6203-2CattleConception rateFertilityGenome-wide association analysis |
| spellingShingle | Jennifer N. Kiser Erin Clancey Joao G. N. Moraes Joseph Dalton Gregory W. Burns Thomas E. Spencer Holly L. Neibergs Identification of loci associated with conception rate in primiparous Holstein cows BMC Genomics Cattle Conception rate Fertility Genome-wide association analysis |
| title | Identification of loci associated with conception rate in primiparous Holstein cows |
| title_full | Identification of loci associated with conception rate in primiparous Holstein cows |
| title_fullStr | Identification of loci associated with conception rate in primiparous Holstein cows |
| title_full_unstemmed | Identification of loci associated with conception rate in primiparous Holstein cows |
| title_short | Identification of loci associated with conception rate in primiparous Holstein cows |
| title_sort | identification of loci associated with conception rate in primiparous holstein cows |
| topic | Cattle Conception rate Fertility Genome-wide association analysis |
| url | http://link.springer.com/article/10.1186/s12864-019-6203-2 |
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