Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method
AIM:To establish a simple and efficient method for the primary culture of rabbit corneal limbus stem cells.<p>METHODS:Obtained the limbal tissues from rabbits,used tissue block and enzyme digestion method to culture the corneal limbus stem cells <i>in vitro</i>.The growth character...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-04-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2018/4/201804007.pdf |
| _version_ | 1819163756063621120 |
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| author | Juan Zhao Dong-Mei Zhan Mo-Chi Yang Xin Fan |
| author_facet | Juan Zhao Dong-Mei Zhan Mo-Chi Yang Xin Fan |
| author_sort | Juan Zhao |
| collection | DOAJ |
| description | AIM:To establish a simple and efficient method for the primary culture of rabbit corneal limbus stem cells.<p>METHODS:Obtained the limbal tissues from rabbits,used tissue block and enzyme digestion method to culture the corneal limbus stem cells <i>in vitro</i>.The growth characteristics of the cultured cells <i>in vitro</i> were observed under inverted microscope.By means of HE,the morphology and construction features of cells were observed.And immunohistochemical method was used to identify the cultured cells.<p>RESULTS:Rabbit corneal limbus stem cells could be fast and simply cultured by using tissue block and enzyme digestion method.The dynamic observation under microscope showed that rabbit corneal limbus stem cells grew well with a higher proliferative capacity.In HE staining,the morphology and structure of cells were normal.AE5 and P63 cellular immune identification were positive.<p>CONCLUSION: Tissue block and enzyme digestion method could be a simple and efficient mode for the primary culture of rabbit corneal limbus stem cells. |
| first_indexed | 2024-12-22T17:49:11Z |
| format | Article |
| id | doaj.art-53006002615f4089b1d72051c52aab59 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-22T17:49:11Z |
| publishDate | 2018-04-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-53006002615f4089b1d72051c52aab592022-12-21T18:18:12ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232018-04-0118462662910.3980/j.issn.1672-5123.2018.4.07Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion methodJuan Zhao0Dong-Mei Zhan1Mo-Chi Yang2Xin Fan3Department of Ophthalmology, the General Hospital of Ningxia Medical University,Yinchuan 750004, Ningxia Hui Autonomous Region,ChinaDepartment of Ophthalmology, the General Hospital of Ningxia Medical University,Yinchuan 750004, Ningxia Hui Autonomous Region,ChinaDepartment of Ophthalmology, the General Hospital of Ningxia Medical University,Yinchuan 750004, Ningxia Hui Autonomous Region,ChinaDepartment of Ophthalmology, the General Hospital of Ningxia Medical University,Yinchuan 750004, Ningxia Hui Autonomous Region,ChinaAIM:To establish a simple and efficient method for the primary culture of rabbit corneal limbus stem cells.<p>METHODS:Obtained the limbal tissues from rabbits,used tissue block and enzyme digestion method to culture the corneal limbus stem cells <i>in vitro</i>.The growth characteristics of the cultured cells <i>in vitro</i> were observed under inverted microscope.By means of HE,the morphology and construction features of cells were observed.And immunohistochemical method was used to identify the cultured cells.<p>RESULTS:Rabbit corneal limbus stem cells could be fast and simply cultured by using tissue block and enzyme digestion method.The dynamic observation under microscope showed that rabbit corneal limbus stem cells grew well with a higher proliferative capacity.In HE staining,the morphology and structure of cells were normal.AE5 and P63 cellular immune identification were positive.<p>CONCLUSION: Tissue block and enzyme digestion method could be a simple and efficient mode for the primary culture of rabbit corneal limbus stem cells.http://ies.ijo.cn/cn_publish/2018/4/201804007.pdfcorneal limbus stem cellscell culturecell identificationcell identification |
| spellingShingle | Juan Zhao Dong-Mei Zhan Mo-Chi Yang Xin Fan Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method Guoji Yanke Zazhi corneal limbus stem cells cell culture cell identification cell identification |
| title | Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| title_full | Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| title_fullStr | Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| title_full_unstemmed | Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| title_short | Preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| title_sort | preliminary exploration of rabbit corneal limbus stem cells cultured with tissue block and enzyme digestion method |
| topic | corneal limbus stem cells cell culture cell identification cell identification |
| url | http://ies.ijo.cn/cn_publish/2018/4/201804007.pdf |
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