Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates
Scratch assays enable the study of the migration process of an injured adherent cell layer in vitro. An apparatus for the reproducible performance of scratch assays and cell harvesting has been developed that meets the requirements for reproducibility in tests as well as easy handling. The entirely...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/20/4414 |
| _version_ | 1818939160351735808 |
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| author | Roman Grimmig Patrick Babczyk Philipp Gillemot Klaus-Peter Schmitz Margit Schulze Edda Tobiasch |
| author_facet | Roman Grimmig Patrick Babczyk Philipp Gillemot Klaus-Peter Schmitz Margit Schulze Edda Tobiasch |
| author_sort | Roman Grimmig |
| collection | DOAJ |
| description | Scratch assays enable the study of the migration process of an injured adherent cell layer in vitro. An apparatus for the reproducible performance of scratch assays and cell harvesting has been developed that meets the requirements for reproducibility in tests as well as easy handling. The entirely autoclavable setup is divided into a sample translation and a scratching system. The translational system is compatible with standard culture dishes and can be modified to adapt to different cell culture systems, while the scratching system can be adjusted according to angle, normal force, shape, and material to adapt to specific questions and demanding substrates. As a result, a fully functional prototype can be presented. This system enables the creation of reproducible and clear scratch edges with a low scratch border roughness within a monolayer of cells. Moreover, the apparatus allows the collection of the migrated cells after scratching for further molecular biological investigations without the need for a second processing step. For comparison, the mechanical properties of manually performed scratch assays are evaluated. |
| first_indexed | 2024-12-20T06:19:20Z |
| format | Article |
| id | doaj.art-5302ae1a6cca4cc18df7e85e8276c100 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-20T06:19:20Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-5302ae1a6cca4cc18df7e85e8276c1002022-12-21T19:50:28ZengMDPI AGApplied Sciences2076-34172019-10-01920441410.3390/app9204414app9204414Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and SubstratesRoman Grimmig0Patrick Babczyk1Philipp Gillemot2Klaus-Peter Schmitz3Margit Schulze4Edda Tobiasch5Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyDepartment of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyDepartment of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyDepartment of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyDepartment of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyDepartment of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, GermanyScratch assays enable the study of the migration process of an injured adherent cell layer in vitro. An apparatus for the reproducible performance of scratch assays and cell harvesting has been developed that meets the requirements for reproducibility in tests as well as easy handling. The entirely autoclavable setup is divided into a sample translation and a scratching system. The translational system is compatible with standard culture dishes and can be modified to adapt to different cell culture systems, while the scratching system can be adjusted according to angle, normal force, shape, and material to adapt to specific questions and demanding substrates. As a result, a fully functional prototype can be presented. This system enables the creation of reproducible and clear scratch edges with a low scratch border roughness within a monolayer of cells. Moreover, the apparatus allows the collection of the migrated cells after scratching for further molecular biological investigations without the need for a second processing step. For comparison, the mechanical properties of manually performed scratch assays are evaluated.https://www.mdpi.com/2076-3417/9/20/4414wound healing assayscratch assaycell harvestingcell migrationprototype apparatusendothelial cells |
| spellingShingle | Roman Grimmig Patrick Babczyk Philipp Gillemot Klaus-Peter Schmitz Margit Schulze Edda Tobiasch Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates Applied Sciences wound healing assay scratch assay cell harvesting cell migration prototype apparatus endothelial cells |
| title | Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates |
| title_full | Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates |
| title_fullStr | Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates |
| title_full_unstemmed | Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates |
| title_short | Development and Evaluation of a Prototype Scratch Apparatus for Wound Assays Adjustable to Different Forces and Substrates |
| title_sort | development and evaluation of a prototype scratch apparatus for wound assays adjustable to different forces and substrates |
| topic | wound healing assay scratch assay cell harvesting cell migration prototype apparatus endothelial cells |
| url | https://www.mdpi.com/2076-3417/9/20/4414 |
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