Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway
Studies have indicated that dietary resveratrol (RES) improves the meat quality of broilers subjected to heat stress (HS), but the mechanism of action remains unclear. Therefore, the main purpose of this study was to investigate the effect of RES on meat quality, muscle antioxidant status, and its m...
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MDPI AG
2022-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/15/1889 |
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| author | Yiyang Zhao Zhen Li Xiaocheng Wang Fei Zhao Chi Wang Qingyue Zhang Xingyong Chen Zhaoyu Geng Cheng Zhang |
| author_facet | Yiyang Zhao Zhen Li Xiaocheng Wang Fei Zhao Chi Wang Qingyue Zhang Xingyong Chen Zhaoyu Geng Cheng Zhang |
| author_sort | Yiyang Zhao |
| collection | DOAJ |
| description | Studies have indicated that dietary resveratrol (RES) improves the meat quality of broilers subjected to heat stress (HS), but the mechanism of action remains unclear. Therefore, the main purpose of this study was to investigate the effect of RES on meat quality, muscle antioxidant status, and its mechanism of action in broilers under HS. A total of 162 male AA broilers at 21 days old with similar weight were randomly assigned to 3 treatment groups with 6 replicates each. The control group (ambient temperature: 22 ± 1 °C) and HS group (ambient temperature: 33 ± 1 °C for 10 h a day from 8:00 to 18:00 and 22 ± 1 °C for the remaining time) were fed a basal diet and the HS + RES group was fed a basal diet with 400 mg/kg RES. The feeding was conducted for 21 continuous days. The results indicated that HS decreased final body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), breast and leg muscle yield, a*<sub>24h</sub>, pH<sub>24h</sub>, the activities of catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GSH-Px), and mRNA levels of nuclear factor erythroid 2–related factor 2 (<i>Nrf2</i>), heme oxygenase-1 (<i>HO-1</i>), NADPH quinone oxidoreductase 1 (<i>NQO1</i>), and <i>GSH-Px</i> (<i>p</i> < 0.05). HS also increased b*<sub>45min</sub>, L*<sub>24h</sub>, drip loss, malondialdehyde (MDA) content, and kelch-like epichlorohydrin-associated protein 1 (<i>Keap1</i>) mRNA level (<i>p</i> < 0.05). Compared with the HS group, the HS + RES group exhibited a higher ADG, breast and leg muscle yield, a*<sub>24h</sub>, pH<sub>24h</sub>, activities of GST and GSH-Px, and mRNA levels of <i>Nrf2</i>, <i>HO-1,</i> and <i>NQO1</i> but had lower drip loss and Keap1 mRNA level (<i>p</i> < 0.05). RES can improve meat quality and the muscle antioxidant ability of heat-stressed broilers by activating the Nrf2 signaling pathway. |
| first_indexed | 2024-03-09T10:10:20Z |
| format | Article |
| id | doaj.art-5302fbd420774244987b9cbd3d2129cd |
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| language | English |
| last_indexed | 2024-03-09T10:10:20Z |
| publishDate | 2022-07-01 |
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| series | Animals |
| spelling | doaj.art-5302fbd420774244987b9cbd3d2129cd2023-12-01T22:48:12ZengMDPI AGAnimals2076-26152022-07-011215188910.3390/ani12151889Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling PathwayYiyang Zhao0Zhen Li1Xiaocheng Wang2Fei Zhao3Chi Wang4Qingyue Zhang5Xingyong Chen6Zhaoyu Geng7Cheng Zhang8Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaStudies have indicated that dietary resveratrol (RES) improves the meat quality of broilers subjected to heat stress (HS), but the mechanism of action remains unclear. Therefore, the main purpose of this study was to investigate the effect of RES on meat quality, muscle antioxidant status, and its mechanism of action in broilers under HS. A total of 162 male AA broilers at 21 days old with similar weight were randomly assigned to 3 treatment groups with 6 replicates each. The control group (ambient temperature: 22 ± 1 °C) and HS group (ambient temperature: 33 ± 1 °C for 10 h a day from 8:00 to 18:00 and 22 ± 1 °C for the remaining time) were fed a basal diet and the HS + RES group was fed a basal diet with 400 mg/kg RES. The feeding was conducted for 21 continuous days. The results indicated that HS decreased final body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), breast and leg muscle yield, a*<sub>24h</sub>, pH<sub>24h</sub>, the activities of catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GSH-Px), and mRNA levels of nuclear factor erythroid 2–related factor 2 (<i>Nrf2</i>), heme oxygenase-1 (<i>HO-1</i>), NADPH quinone oxidoreductase 1 (<i>NQO1</i>), and <i>GSH-Px</i> (<i>p</i> < 0.05). HS also increased b*<sub>45min</sub>, L*<sub>24h</sub>, drip loss, malondialdehyde (MDA) content, and kelch-like epichlorohydrin-associated protein 1 (<i>Keap1</i>) mRNA level (<i>p</i> < 0.05). Compared with the HS group, the HS + RES group exhibited a higher ADG, breast and leg muscle yield, a*<sub>24h</sub>, pH<sub>24h</sub>, activities of GST and GSH-Px, and mRNA levels of <i>Nrf2</i>, <i>HO-1,</i> and <i>NQO1</i> but had lower drip loss and Keap1 mRNA level (<i>p</i> < 0.05). RES can improve meat quality and the muscle antioxidant ability of heat-stressed broilers by activating the Nrf2 signaling pathway.https://www.mdpi.com/2076-2615/12/15/1889antioxidantheat stressresveratrolmeat qualitysignaling pathway |
| spellingShingle | Yiyang Zhao Zhen Li Xiaocheng Wang Fei Zhao Chi Wang Qingyue Zhang Xingyong Chen Zhaoyu Geng Cheng Zhang Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway Animals antioxidant heat stress resveratrol meat quality signaling pathway |
| title | Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway |
| title_full | Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway |
| title_fullStr | Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway |
| title_full_unstemmed | Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway |
| title_short | Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway |
| title_sort | resveratrol attenuates heat stress induced impairment of meat quality in broilers by regulating the nrf2 signaling pathway |
| topic | antioxidant heat stress resveratrol meat quality signaling pathway |
| url | https://www.mdpi.com/2076-2615/12/15/1889 |
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