Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping
Chromatin looping plays an important role in gene regulation and the ability to manipulate loops would aid in understanding how this occurs. Here the authors present CLOuD9, a system that uses dimerized Cas9 complexes to selectively and reversibly establish chromatin loops.
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms15993 |
| _version_ | 1818990708175929344 |
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| author | Stefanie L. Morgan Natasha C. Mariano Abel Bermudez Nicole L. Arruda Fangting Wu Yunhai Luo Gautam Shankar Lin Jia Huiling Chen Ji-Fan Hu Andrew R. Hoffman Chiao-Chain Huang Sharon J. Pitteri Kevin C. Wang |
| author_facet | Stefanie L. Morgan Natasha C. Mariano Abel Bermudez Nicole L. Arruda Fangting Wu Yunhai Luo Gautam Shankar Lin Jia Huiling Chen Ji-Fan Hu Andrew R. Hoffman Chiao-Chain Huang Sharon J. Pitteri Kevin C. Wang |
| author_sort | Stefanie L. Morgan |
| collection | DOAJ |
| description | Chromatin looping plays an important role in gene regulation and the ability to manipulate loops would aid in understanding how this occurs. Here the authors present CLOuD9, a system that uses dimerized Cas9 complexes to selectively and reversibly establish chromatin loops. |
| first_indexed | 2024-12-20T19:58:40Z |
| format | Article |
| id | doaj.art-5309d42ed5b04e42a18e02ba03f78364 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T19:58:40Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-5309d42ed5b04e42a18e02ba03f783642022-12-21T19:28:06ZengNature PortfolioNature Communications2041-17232017-07-01811910.1038/ncomms15993Manipulation of nuclear architecture through CRISPR-mediated chromosomal loopingStefanie L. Morgan0Natasha C. Mariano1Abel Bermudez2Nicole L. Arruda3Fangting Wu4Yunhai Luo5Gautam Shankar6Lin Jia7Huiling Chen8Ji-Fan Hu9Andrew R. Hoffman10Chiao-Chain Huang11Sharon J. Pitteri12Kevin C. Wang13Department of Dermatology, Program in Epithelial Biology, Stanford University School of MedicineDepartment of Dermatology, Program in Epithelial Biology, Stanford University School of MedicineDepartment of Radiology, Canary Center for Cancer Early Detection, Stanford University School of MedicineDepartment of Biology, Bridgewater State UniversitySystem BiosciencesDepartment of Dermatology, Program in Epithelial Biology, Stanford University School of MedicineDepartment of Dermatology, Program in Epithelial Biology, Stanford University School of MedicineVeterans Affairs Healthcare SystemVeterans Affairs Healthcare SystemVeterans Affairs Healthcare SystemVeterans Affairs Healthcare SystemSystem BiosciencesDepartment of Radiology, Canary Center for Cancer Early Detection, Stanford University School of MedicineDepartment of Dermatology, Program in Epithelial Biology, Stanford University School of MedicineChromatin looping plays an important role in gene regulation and the ability to manipulate loops would aid in understanding how this occurs. Here the authors present CLOuD9, a system that uses dimerized Cas9 complexes to selectively and reversibly establish chromatin loops.https://doi.org/10.1038/ncomms15993 |
| spellingShingle | Stefanie L. Morgan Natasha C. Mariano Abel Bermudez Nicole L. Arruda Fangting Wu Yunhai Luo Gautam Shankar Lin Jia Huiling Chen Ji-Fan Hu Andrew R. Hoffman Chiao-Chain Huang Sharon J. Pitteri Kevin C. Wang Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping Nature Communications |
| title | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_full | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_fullStr | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_full_unstemmed | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_short | Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping |
| title_sort | manipulation of nuclear architecture through crispr mediated chromosomal looping |
| url | https://doi.org/10.1038/ncomms15993 |
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