Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices
A novel conductive composite based on PEDOT:PSS, BSA, and Nafion for effective immobilization of acetic acid bacteria on graphite electrodes as part of biosensors and microbial fuel cells has been proposed. It is shown that individual components in the composite do not have a significant negative ef...
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MDPI AG
2024-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/24/3/905 |
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| author | Sergei E. Tarasov Yulia V. Plekhanova Aleksandr G. Bykov Konstantin V. Kadison Anastasia S. Medvedeva Anatoly N. Reshetilov Vyacheslav A. Arlyapov |
| author_facet | Sergei E. Tarasov Yulia V. Plekhanova Aleksandr G. Bykov Konstantin V. Kadison Anastasia S. Medvedeva Anatoly N. Reshetilov Vyacheslav A. Arlyapov |
| author_sort | Sergei E. Tarasov |
| collection | DOAJ |
| description | A novel conductive composite based on PEDOT:PSS, BSA, and Nafion for effective immobilization of acetic acid bacteria on graphite electrodes as part of biosensors and microbial fuel cells has been proposed. It is shown that individual components in the composite do not have a significant negative effect on the catalytic activity of microorganisms during prolonged contact. The values of heterogeneous electron transport constants in the presence of two types of water-soluble mediators were calculated. The use of the composite as part of a microbial biosensor resulted in an electrode operating for more than 140 days. Additional modification of carbon electrodes with nanomaterial allowed to increase the sensitivity to glucose from 1.48 to 2.81 μA × mM<sup>−1</sup> × cm<sup>−2</sup> without affecting the affinity of bacterial enzyme complexes to the substrate. Cells in the presented composite, as part of a microbial fuel cell based on electrodes from thermally expanded graphite, retained the ability to generate electricity for more than 120 days using glucose solution as well as vegetable extract solutions as carbon sources. The obtained data expand the understanding of the composition of possible matrices for the immobilization of <i>Gluconobacter</i> bacteria and may be useful in the development of biosensors and biofuel cells. |
| first_indexed | 2024-03-08T03:48:43Z |
| format | Article |
| id | doaj.art-530a46e1ad494b96a0f994733ba3f696 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-08T03:48:43Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-530a46e1ad494b96a0f994733ba3f6962024-02-09T15:22:12ZengMDPI AGSensors1424-82202024-01-0124390510.3390/s24030905Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical DevicesSergei E. Tarasov0Yulia V. Plekhanova1Aleksandr G. Bykov2Konstantin V. Kadison3Anastasia S. Medvedeva4Anatoly N. Reshetilov5Vyacheslav A. Arlyapov6G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, 142290 Moscow, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, 142290 Moscow, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, 142290 Moscow, RussiaFederal State Budgetary Educational Institution of Higher Education, Tula State University, 300012 Tula, RussiaFederal State Budgetary Educational Institution of Higher Education, Tula State University, 300012 Tula, RussiaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, 142290 Moscow, RussiaFederal State Budgetary Educational Institution of Higher Education, Tula State University, 300012 Tula, RussiaA novel conductive composite based on PEDOT:PSS, BSA, and Nafion for effective immobilization of acetic acid bacteria on graphite electrodes as part of biosensors and microbial fuel cells has been proposed. It is shown that individual components in the composite do not have a significant negative effect on the catalytic activity of microorganisms during prolonged contact. The values of heterogeneous electron transport constants in the presence of two types of water-soluble mediators were calculated. The use of the composite as part of a microbial biosensor resulted in an electrode operating for more than 140 days. Additional modification of carbon electrodes with nanomaterial allowed to increase the sensitivity to glucose from 1.48 to 2.81 μA × mM<sup>−1</sup> × cm<sup>−2</sup> without affecting the affinity of bacterial enzyme complexes to the substrate. Cells in the presented composite, as part of a microbial fuel cell based on electrodes from thermally expanded graphite, retained the ability to generate electricity for more than 120 days using glucose solution as well as vegetable extract solutions as carbon sources. The obtained data expand the understanding of the composition of possible matrices for the immobilization of <i>Gluconobacter</i> bacteria and may be useful in the development of biosensors and biofuel cells.https://www.mdpi.com/1424-8220/24/3/905conductive compositePEDOT:PSSNafionbovine serum albuminmicrobial biosensormicrobial fuel cell |
| spellingShingle | Sergei E. Tarasov Yulia V. Plekhanova Aleksandr G. Bykov Konstantin V. Kadison Anastasia S. Medvedeva Anatoly N. Reshetilov Vyacheslav A. Arlyapov Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices Sensors conductive composite PEDOT:PSS Nafion bovine serum albumin microbial biosensor microbial fuel cell |
| title | Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices |
| title_full | Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices |
| title_fullStr | Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices |
| title_full_unstemmed | Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices |
| title_short | Novel Conductive Polymer Composite PEDOT:PSS/Bovine Serum Albumin for Microbial Bioelectrochemical Devices |
| title_sort | novel conductive polymer composite pedot pss bovine serum albumin for microbial bioelectrochemical devices |
| topic | conductive composite PEDOT:PSS Nafion bovine serum albumin microbial biosensor microbial fuel cell |
| url | https://www.mdpi.com/1424-8220/24/3/905 |
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