Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae
Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such “orphan” genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2014-12-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262289 |
| _version_ | 1818292309732622336 |
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| author | Abu Sadat Junhyun Jeon Albely Afifa Mir Seongbeom Kim Jaeyoung Choi Yong-Hwan Lee |
| author_facet | Abu Sadat Junhyun Jeon Albely Afifa Mir Seongbeom Kim Jaeyoung Choi Yong-Hwan Lee |
| author_sort | Abu Sadat |
| collection | DOAJ |
| description | Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such “orphan” genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection. |
| first_indexed | 2024-12-13T02:57:55Z |
| format | Article |
| id | doaj.art-530d3e5010a54bb0a2f8fccd784fa9d9 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-13T02:57:55Z |
| publishDate | 2014-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-530d3e5010a54bb0a2f8fccd784fa9d92022-12-22T00:01:55ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542014-12-0130436737410.5423/PPJ.OA.08.2014.0072PPJ.OA.08.2014.0072Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzaeAbu Sadat0Junhyun Jeon1Albely Afifa Mir2Seongbeom Kim3Jaeyoung Choi4Yong-Hwan Lee5Department of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaDepartment of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaDepartment of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaDepartment of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaDepartment of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaDepartment of Agricultural Biotechnology, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaGenomes contain a large number of unique genes which have not been found in other species. Although the origin of such “orphan” genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262289fungal pathogenesisgene birthMagnaporthe oryzaeorphan gene |
| spellingShingle | Abu Sadat Junhyun Jeon Albely Afifa Mir Seongbeom Kim Jaeyoung Choi Yong-Hwan Lee Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae The Plant Pathology Journal fungal pathogenesis gene birth Magnaporthe oryzae orphan gene |
| title | Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| title_full | Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| title_fullStr | Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| title_full_unstemmed | Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| title_short | Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| title_sort | analysis of in planta expressed orphan genes in the rice blast fungus magnaporthe oryzae |
| topic | fungal pathogenesis gene birth Magnaporthe oryzae orphan gene |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262289 |
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