A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin
A new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light sc...
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2021-06-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/13/3517 |
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| author | Mariia Chernykh Dmytro Zavalny Viktoriya Sokolova Stanislav Ponomarenko Svitlana Prylutska Yuliia Kuziv Vasyl Chumachenko Andrii Marynin Nataliya Kutsevol Matthias Epple Uwe Ritter Jacek Piosik Yuriy Prylutskyy |
| author_facet | Mariia Chernykh Dmytro Zavalny Viktoriya Sokolova Stanislav Ponomarenko Svitlana Prylutska Yuliia Kuziv Vasyl Chumachenko Andrii Marynin Nataliya Kutsevol Matthias Epple Uwe Ritter Jacek Piosik Yuriy Prylutskyy |
| author_sort | Mariia Chernykh |
| collection | DOAJ |
| description | A new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) spectroscopy). The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa) as a model for cancer cells was studied using MTT and Live/Dead assays after incubation with a D-g-PNIPAM copolymer as a carrier for the drug doxorubicin (Dox) as well as a D-g-PNIPAM + Dox mixture as a function of the concentration. FTIR spectroscopy clearly indicated the complex formation of Dox with the D-g-PNIPAM copolymer. The size distribution of particles in Hank’s solution was determined by the DLS technique at different temperatures. The in vitro uptake of the studied D-g-PNIPAM + Dox nanoparticles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PNIPAM + Dox nanoparticles in contrast to Dox alone showed higher toxicity toward cancer cells. All of the aforementioned facts indicate a possibility of further preclinical studies of the water-soluble D-g-PNIPAM particles’ behavior in animal tumor models in vivo as promising carriers of anticancer agents. |
| first_indexed | 2024-03-10T10:05:29Z |
| format | Article |
| id | doaj.art-530f40071b4341669a8b23a139b70b53 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T10:05:29Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-530f40071b4341669a8b23a139b70b532023-11-22T01:33:19ZengMDPI AGMaterials1996-19442021-06-011413351710.3390/ma14133517A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug DoxorubicinMariia Chernykh0Dmytro Zavalny1Viktoriya Sokolova2Stanislav Ponomarenko3Svitlana Prylutska4Yuliia Kuziv5Vasyl Chumachenko6Andrii Marynin7Nataliya Kutsevol8Matthias Epple9Uwe Ritter10Jacek Piosik11Yuriy Prylutskyy12Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineCenter for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, GermanyTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineDepartment of Physiology, Plant Biochemistry and Bioenergetics, National University of Life and Environmental Science of Ukraine, Heroiv Oborony Street, 15, 03041 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineNational University of Food Technologies of Ukraine, Volodymyrska Street, 01033 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineCenter for Nanointegration Duisburg-Essen (CeNIDE), Institute of Inorganic Chemistry, University of Duisburg-Essen, University Street, 5-7, 45117 Essen, GermanyInstitute of Chemistry and Biotechnology, Technical University of Ilmenau, Weimarer Street, 25, 98693 Ilmenau, GermanyLaboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Abrahama 58, 80-307 Gdańsk, PolandTaras Shevchenko National University of Kyiv, Volodymyrska Street, 64, 01601 Kyiv, UkraineA new water-soluble thermosensitive star-like copolymer, dextran-graft-poly-N-iso-propilacrylamide (D-g-PNIPAM), was created and characterized by various techniques (size-exclusion chromatography, differential scanning calorimetry, Fourier-transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) spectroscopy). The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa) as a model for cancer cells was studied using MTT and Live/Dead assays after incubation with a D-g-PNIPAM copolymer as a carrier for the drug doxorubicin (Dox) as well as a D-g-PNIPAM + Dox mixture as a function of the concentration. FTIR spectroscopy clearly indicated the complex formation of Dox with the D-g-PNIPAM copolymer. The size distribution of particles in Hank’s solution was determined by the DLS technique at different temperatures. The in vitro uptake of the studied D-g-PNIPAM + Dox nanoparticles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PNIPAM + Dox nanoparticles in contrast to Dox alone showed higher toxicity toward cancer cells. All of the aforementioned facts indicate a possibility of further preclinical studies of the water-soluble D-g-PNIPAM particles’ behavior in animal tumor models in vivo as promising carriers of anticancer agents.https://www.mdpi.com/1996-1944/14/13/3517thermosensitive star-like copolymerdoxorubicinHeLa cellscytotoxicityin vitro uptake |
| spellingShingle | Mariia Chernykh Dmytro Zavalny Viktoriya Sokolova Stanislav Ponomarenko Svitlana Prylutska Yuliia Kuziv Vasyl Chumachenko Andrii Marynin Nataliya Kutsevol Matthias Epple Uwe Ritter Jacek Piosik Yuriy Prylutskyy A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin Materials thermosensitive star-like copolymer doxorubicin HeLa cells cytotoxicity in vitro uptake |
| title | A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin |
| title_full | A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin |
| title_fullStr | A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin |
| title_full_unstemmed | A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin |
| title_short | A New Water-Soluble Thermosensitive Star-Like Copolymer as a Promising Carrier of the Chemotherapeutic Drug Doxorubicin |
| title_sort | new water soluble thermosensitive star like copolymer as a promising carrier of the chemotherapeutic drug doxorubicin |
| topic | thermosensitive star-like copolymer doxorubicin HeLa cells cytotoxicity in vitro uptake |
| url | https://www.mdpi.com/1996-1944/14/13/3517 |
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