Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.)
Nowadays, microorganisms that display plant growth promoting properties are significantly interesting for their potential role in reducing the use of chemical fertilizers. This research study proposed the isolation of the actinobacteria associated with arbuscular mycorrhizal fungi (AMF) spores and t...
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1274 |
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| author | Leardwiriyakool Chaiya Jaturong Kumla Nakarin Suwannarach Tanongkiat Kiatsiriroat Saisamorn Lumyong |
| author_facet | Leardwiriyakool Chaiya Jaturong Kumla Nakarin Suwannarach Tanongkiat Kiatsiriroat Saisamorn Lumyong |
| author_sort | Leardwiriyakool Chaiya |
| collection | DOAJ |
| description | Nowadays, microorganisms that display plant growth promoting properties are significantly interesting for their potential role in reducing the use of chemical fertilizers. This research study proposed the isolation of the actinobacteria associated with arbuscular mycorrhizal fungi (AMF) spores and the investigation of their plant growth promoting properties in the in vitro assay. Three actinobacterial strains were obtained and identified to the genus <i>Streptomyces</i> (GETU-1 and GIG-1) and <i>Amycolatopsis</i> (GLM-2). The results indicated that all actinobacterial strains produced indole-3-acetic acid (IAA) and were positive in terms of siderophore, endoglucanase, and ammonia productions. In the in vitro assay, all strains were grown in the presence of water activity within a range of 0.897 to 0.998, pH values within a range of 5–11, and in the presence of 2.5% NaCl for the investigation of drought, pH, and salt tolerances, respectively. Additionally, all strains were able to tolerate commercial insecticides (propargite and methomyl) and fungicides (captan) at the recommended dosages for field applications. Only, <i>Amycolatopsis</i> sp. GLM-2 showed tolerance to benomyl at the recommended dose. All the obtained actinobacteria were characterized as plant growth promoting strains by improving the growth of chili plants (<i>Capsicum flutescens</i> L.). Moreover, the co-inoculation treatment of the obtained actinobacteria and AMF (<i>Claroideoglomus etunicatum</i>) spores could significantly increase plant growth, contribute to the chlorophyll index, and enhance fruit production in chili plants. Additionally, the highest value of AMF spore production and the greatest percentage of root colonization were observed in the treatment that had been co-inoculated with <i>Streptomyces</i> sp. GETU-1. |
| first_indexed | 2024-03-10T10:30:12Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:30:12Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-53119ba4468047888360f83c24f833922023-11-21T23:41:19ZengMDPI AGMicroorganisms2076-26072021-06-0196127410.3390/microorganisms9061274Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.)Leardwiriyakool Chaiya0Jaturong Kumla1Nakarin Suwannarach2Tanongkiat Kiatsiriroat3Saisamorn Lumyong4Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandResearch Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandResearch Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Mechanical Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, ThailandResearch Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandNowadays, microorganisms that display plant growth promoting properties are significantly interesting for their potential role in reducing the use of chemical fertilizers. This research study proposed the isolation of the actinobacteria associated with arbuscular mycorrhizal fungi (AMF) spores and the investigation of their plant growth promoting properties in the in vitro assay. Three actinobacterial strains were obtained and identified to the genus <i>Streptomyces</i> (GETU-1 and GIG-1) and <i>Amycolatopsis</i> (GLM-2). The results indicated that all actinobacterial strains produced indole-3-acetic acid (IAA) and were positive in terms of siderophore, endoglucanase, and ammonia productions. In the in vitro assay, all strains were grown in the presence of water activity within a range of 0.897 to 0.998, pH values within a range of 5–11, and in the presence of 2.5% NaCl for the investigation of drought, pH, and salt tolerances, respectively. Additionally, all strains were able to tolerate commercial insecticides (propargite and methomyl) and fungicides (captan) at the recommended dosages for field applications. Only, <i>Amycolatopsis</i> sp. GLM-2 showed tolerance to benomyl at the recommended dose. All the obtained actinobacteria were characterized as plant growth promoting strains by improving the growth of chili plants (<i>Capsicum flutescens</i> L.). Moreover, the co-inoculation treatment of the obtained actinobacteria and AMF (<i>Claroideoglomus etunicatum</i>) spores could significantly increase plant growth, contribute to the chlorophyll index, and enhance fruit production in chili plants. Additionally, the highest value of AMF spore production and the greatest percentage of root colonization were observed in the treatment that had been co-inoculated with <i>Streptomyces</i> sp. GETU-1.https://www.mdpi.com/2076-2607/9/6/1274bio-inoculantco-inoculationplant growth promoting propertiessolanaceous plants |
| spellingShingle | Leardwiriyakool Chaiya Jaturong Kumla Nakarin Suwannarach Tanongkiat Kiatsiriroat Saisamorn Lumyong Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) Microorganisms bio-inoculant co-inoculation plant growth promoting properties solanaceous plants |
| title | Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) |
| title_full | Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) |
| title_fullStr | Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) |
| title_full_unstemmed | Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) |
| title_short | Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (<i>Capsicum flutescens</i> L.) |
| title_sort | isolation characterization and efficacy of actinobacteria associated with arbuscular mycorrhizal spores in promoting plant growth of chili i capsicum flutescens i l |
| topic | bio-inoculant co-inoculation plant growth promoting properties solanaceous plants |
| url | https://www.mdpi.com/2076-2607/9/6/1274 |
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