The peroxisomal protein import machinery displays a preference for monomeric substrates
Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and transported by the shuttling receptor PEX5 to the peroxisomal membrane docking/translocation machinery, where they are translocated into the organelle matrix. Under certain experimental conditions this protein import machinery ha...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2015-01-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.140236 |
| _version_ | 1818939036308340736 |
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| author | Marta O. Freitas Tânia Francisco Tony A. Rodrigues Celien Lismont Pedro Domingues Manuel P. Pinto Cláudia P. Grou Marc Fransen Jorge E. Azevedo |
| author_facet | Marta O. Freitas Tânia Francisco Tony A. Rodrigues Celien Lismont Pedro Domingues Manuel P. Pinto Cláudia P. Grou Marc Fransen Jorge E. Azevedo |
| author_sort | Marta O. Freitas |
| collection | DOAJ |
| description | Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and transported by the shuttling receptor PEX5 to the peroxisomal membrane docking/translocation machinery, where they are translocated into the organelle matrix. Under certain experimental conditions this protein import machinery has the remarkable capacity to accept already oligomerized proteins, a property that has heavily influenced current models on the mechanism of peroxisomal protein import. However, whether or not oligomeric proteins are really the best and most frequent clients of this machinery remain unclear. In this work, we present three lines of evidence suggesting that the peroxisomal import machinery displays a preference for monomeric proteins. First, in agreement with previous findings on catalase, we show that PEX5 binds newly synthesized (monomeric) acyl-CoA oxidase 1 (ACOX1) and urate oxidase (UOX), potently inhibiting their oligomerization. Second, in vitro import experiments suggest that monomeric ACOX1 and UOX are better peroxisomal import substrates than the corresponding oligomeric forms. Finally, we provide data strongly suggesting that although ACOX1 lacking a peroxisomal targeting signal can be imported into peroxisomes when co-expressed with ACOX1 containing its targeting signal, this import pathway is inefficient. |
| first_indexed | 2024-12-20T06:17:22Z |
| format | Article |
| id | doaj.art-53120c0e99fa49b2ab2557d4c9bff18f |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-20T06:17:22Z |
| publishDate | 2015-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-53120c0e99fa49b2ab2557d4c9bff18f2022-12-21T19:50:30ZengThe Royal SocietyOpen Biology2046-24412015-01-015410.1098/rsob.140236140236The peroxisomal protein import machinery displays a preference for monomeric substratesMarta O. FreitasTânia FranciscoTony A. RodriguesCelien LismontPedro DominguesManuel P. PintoCláudia P. GrouMarc FransenJorge E. AzevedoPeroxisomal matrix proteins are synthesized on cytosolic ribosomes and transported by the shuttling receptor PEX5 to the peroxisomal membrane docking/translocation machinery, where they are translocated into the organelle matrix. Under certain experimental conditions this protein import machinery has the remarkable capacity to accept already oligomerized proteins, a property that has heavily influenced current models on the mechanism of peroxisomal protein import. However, whether or not oligomeric proteins are really the best and most frequent clients of this machinery remain unclear. In this work, we present three lines of evidence suggesting that the peroxisomal import machinery displays a preference for monomeric proteins. First, in agreement with previous findings on catalase, we show that PEX5 binds newly synthesized (monomeric) acyl-CoA oxidase 1 (ACOX1) and urate oxidase (UOX), potently inhibiting their oligomerization. Second, in vitro import experiments suggest that monomeric ACOX1 and UOX are better peroxisomal import substrates than the corresponding oligomeric forms. Finally, we provide data strongly suggesting that although ACOX1 lacking a peroxisomal targeting signal can be imported into peroxisomes when co-expressed with ACOX1 containing its targeting signal, this import pathway is inefficient.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.140236peroxisomespex5docking/translocation machineryacyl-coa oxidaseurate oxidaseprotein import |
| spellingShingle | Marta O. Freitas Tânia Francisco Tony A. Rodrigues Celien Lismont Pedro Domingues Manuel P. Pinto Cláudia P. Grou Marc Fransen Jorge E. Azevedo The peroxisomal protein import machinery displays a preference for monomeric substrates Open Biology peroxisomes pex5 docking/translocation machinery acyl-coa oxidase urate oxidase protein import |
| title | The peroxisomal protein import machinery displays a preference for monomeric substrates |
| title_full | The peroxisomal protein import machinery displays a preference for monomeric substrates |
| title_fullStr | The peroxisomal protein import machinery displays a preference for monomeric substrates |
| title_full_unstemmed | The peroxisomal protein import machinery displays a preference for monomeric substrates |
| title_short | The peroxisomal protein import machinery displays a preference for monomeric substrates |
| title_sort | peroxisomal protein import machinery displays a preference for monomeric substrates |
| topic | peroxisomes pex5 docking/translocation machinery acyl-coa oxidase urate oxidase protein import |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.140236 |
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