BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients
SARS-CoV-2 vaccination is the standard of care for the prevention of COVID-19 disease. Although vaccination triggers both humoral and cellular immune response, COVID-19 vaccination efficacy is currently evaluated by measuring antibodies only, whereas adaptative cellular immunity is unexplored. Our a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/8/1659 |
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| author | Evelina La Civita Carla Zannella Stefano Brusa Paolo Romano Elisa Schettino Fabrizio Salemi Rosa Carrano Luca Gentile Alessandra Punziano Gianluca Lagnese Giuseppe Spadaro Gianluigi Franci Massimiliano Galdiero Daniela Terracciano Giuseppe Portella Stefania Loffredo |
| author_facet | Evelina La Civita Carla Zannella Stefano Brusa Paolo Romano Elisa Schettino Fabrizio Salemi Rosa Carrano Luca Gentile Alessandra Punziano Gianluca Lagnese Giuseppe Spadaro Gianluigi Franci Massimiliano Galdiero Daniela Terracciano Giuseppe Portella Stefania Loffredo |
| author_sort | Evelina La Civita |
| collection | DOAJ |
| description | SARS-CoV-2 vaccination is the standard of care for the prevention of COVID-19 disease. Although vaccination triggers both humoral and cellular immune response, COVID-19 vaccination efficacy is currently evaluated by measuring antibodies only, whereas adaptative cellular immunity is unexplored. Our aim is to test humoral and cell-mediated response after three doses of BNT162b vaccine in two cohorts of fragile patients: Common Variable Immunodeficiency (CVID) patients and Kidney Transplant Recipients (KTR) patients compared to healthy donors. We enrolled 10 healthy controls (HCs), 19 CVID patients and 17 KTR patients. HC BNT162b third dose had successfully mounted humoral immune response. A positive correlation between Anti-Spike Trimeric IgG concentration and neutralizing antibody titer was also observed. CVID and KTR groups showed a lower humoral immune response compared to HCs. IFN-γ release induced by epitopes of the Spike protein in stimulated CD4<sup>+</sup> and CD8<sup>+</sup> T cells was similar among vaccinated HC, CVID and KTR. Patients vaccinated and infected showed a more efficient humoral and cell-mediated response compared to only vaccinated patients. In conclusion, CVID and KTR patients had an efficient cell-mediated but not humoral response to SARS-CoV-2 vaccine, suggesting that the evaluation of T cell responses could be a more sensitive marker of immunization in these subjects. |
| first_indexed | 2024-03-10T23:30:35Z |
| format | Article |
| id | doaj.art-53123d8e31a84a10a777ca974e598e28 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T23:30:35Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-53123d8e31a84a10a777ca974e598e282023-11-19T03:19:51ZengMDPI AGViruses1999-49152023-07-01158165910.3390/v15081659BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency PatientsEvelina La Civita0Carla Zannella1Stefano Brusa2Paolo Romano3Elisa Schettino4Fabrizio Salemi5Rosa Carrano6Luca Gentile7Alessandra Punziano8Gianluca Lagnese9Giuseppe Spadaro10Gianluigi Franci11Massimiliano Galdiero12Daniela Terracciano13Giuseppe Portella14Stefania Loffredo15Department of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Public Health, Section of Nephrology, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Public Health, Section of Nephrology, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Public Health, Section of Nephrology, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Public Health, Section of Nephrology, University of Naples “Federico II”, 80131 Naples, ItalyIntegrated Department of Laboratory and Trasfusion Medicine, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Medicine, Surgery and Dentistry “ScholaMedicaSalernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalySARS-CoV-2 vaccination is the standard of care for the prevention of COVID-19 disease. Although vaccination triggers both humoral and cellular immune response, COVID-19 vaccination efficacy is currently evaluated by measuring antibodies only, whereas adaptative cellular immunity is unexplored. Our aim is to test humoral and cell-mediated response after three doses of BNT162b vaccine in two cohorts of fragile patients: Common Variable Immunodeficiency (CVID) patients and Kidney Transplant Recipients (KTR) patients compared to healthy donors. We enrolled 10 healthy controls (HCs), 19 CVID patients and 17 KTR patients. HC BNT162b third dose had successfully mounted humoral immune response. A positive correlation between Anti-Spike Trimeric IgG concentration and neutralizing antibody titer was also observed. CVID and KTR groups showed a lower humoral immune response compared to HCs. IFN-γ release induced by epitopes of the Spike protein in stimulated CD4<sup>+</sup> and CD8<sup>+</sup> T cells was similar among vaccinated HC, CVID and KTR. Patients vaccinated and infected showed a more efficient humoral and cell-mediated response compared to only vaccinated patients. In conclusion, CVID and KTR patients had an efficient cell-mediated but not humoral response to SARS-CoV-2 vaccine, suggesting that the evaluation of T cell responses could be a more sensitive marker of immunization in these subjects.https://www.mdpi.com/1999-4915/15/8/1659mRNA vaccineimmunodeficiencyhumoral immunitycell-mediated immunity |
| spellingShingle | Evelina La Civita Carla Zannella Stefano Brusa Paolo Romano Elisa Schettino Fabrizio Salemi Rosa Carrano Luca Gentile Alessandra Punziano Gianluca Lagnese Giuseppe Spadaro Gianluigi Franci Massimiliano Galdiero Daniela Terracciano Giuseppe Portella Stefania Loffredo BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients Viruses mRNA vaccine immunodeficiency humoral immunity cell-mediated immunity |
| title | BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients |
| title_full | BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients |
| title_fullStr | BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients |
| title_full_unstemmed | BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients |
| title_short | BNT162b2 Elicited an Efficient Cell-Mediated Response against SARS-CoV-2 in Kidney Transplant Recipients and Common Variable Immunodeficiency Patients |
| title_sort | bnt162b2 elicited an efficient cell mediated response against sars cov 2 in kidney transplant recipients and common variable immunodeficiency patients |
| topic | mRNA vaccine immunodeficiency humoral immunity cell-mediated immunity |
| url | https://www.mdpi.com/1999-4915/15/8/1659 |
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