Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma
Ethyl acetate fraction column chromatographic analysis was used to isolate eleven compounds (numerically tagged 1–10) from Cassia occidentalis L. in this study. Two unique metabolites, including a neolignan compound designated as occidentalignan I (9) and a flavonoidal glycoside, chrysin-7-O-α-L-rha...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2023-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/6111058 |
| _version_ | 1827010053491654656 |
|---|---|
| author | Hanaa M. Sayed Mahmoud A. Ramadan Heba H. Salem Marwa A. A. Fayed |
| author_facet | Hanaa M. Sayed Mahmoud A. Ramadan Heba H. Salem Marwa A. A. Fayed |
| author_sort | Hanaa M. Sayed |
| collection | DOAJ |
| description | Ethyl acetate fraction column chromatographic analysis was used to isolate eleven compounds (numerically tagged 1–10) from Cassia occidentalis L. in this study. Two unique metabolites, including a neolignan compound designated as occidentalignan I (9) and a flavonoidal glycoside, chrysin-7-O-α-L-rhamnopyranosyl (10), were identified, while silybin A (8) was the first flavonolignan to be isolated from the Fabaceae family. Four compounds, including β-sitosterol-3-O-β-D-glucopyranoside (1), stigmasterol-3-O-β-D-glucopyranoside (2), betulinic acid (3), and vanillic acid (4) were isolated from C. occidentalis for the first time. In addition, four known compounds, cinnamic acid (5), p-hydroxybenzoic acid (6), β- resorcylic acid (7), and citric acid (11), were also detected. The in-vitro cytotoxicity assessment of the methanolic extract of C. occidentalis on seven cancer cell lines, including A-549, Colo-205, Huh-7, HCT-116, PANC-1, SKOV-3, and BNL, demonstrated its selective potent cytotoxicity on lung cancer cells without affecting normal BNL cells. In contrast, the methanolic extract showed moderate activity on Colo-205 and Huh-7 and nearly no activity on HCT-116, PANC-1, and SKOV-3 cell lines. These results suggest that the methanolic extract of C. occidentalis is an excellent candidate with potential antiproliferative activity against lung cancer; however, further studies are necessary to clarify its mechanism of action. |
| first_indexed | 2024-04-09T17:51:23Z |
| format | Article |
| id | doaj.art-531417dff1de473aa98955a5411069ac |
| institution | Directory Open Access Journal |
| issn | 2090-9071 |
| language | English |
| last_indexed | 2025-02-18T13:00:06Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj.art-531417dff1de473aa98955a5411069ac2024-11-02T03:56:08ZengHindawi LimitedJournal of Chemistry2090-90712023-01-01202310.1155/2023/6111058Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung CarcinomaHanaa M. Sayed0Mahmoud A. Ramadan1Heba H. Salem2Marwa A. A. Fayed3Department of PharmacognosyDepartment of PharmacognosyDepartment of PharmacognosyDepartment of PharmacognosyEthyl acetate fraction column chromatographic analysis was used to isolate eleven compounds (numerically tagged 1–10) from Cassia occidentalis L. in this study. Two unique metabolites, including a neolignan compound designated as occidentalignan I (9) and a flavonoidal glycoside, chrysin-7-O-α-L-rhamnopyranosyl (10), were identified, while silybin A (8) was the first flavonolignan to be isolated from the Fabaceae family. Four compounds, including β-sitosterol-3-O-β-D-glucopyranoside (1), stigmasterol-3-O-β-D-glucopyranoside (2), betulinic acid (3), and vanillic acid (4) were isolated from C. occidentalis for the first time. In addition, four known compounds, cinnamic acid (5), p-hydroxybenzoic acid (6), β- resorcylic acid (7), and citric acid (11), were also detected. The in-vitro cytotoxicity assessment of the methanolic extract of C. occidentalis on seven cancer cell lines, including A-549, Colo-205, Huh-7, HCT-116, PANC-1, SKOV-3, and BNL, demonstrated its selective potent cytotoxicity on lung cancer cells without affecting normal BNL cells. In contrast, the methanolic extract showed moderate activity on Colo-205 and Huh-7 and nearly no activity on HCT-116, PANC-1, and SKOV-3 cell lines. These results suggest that the methanolic extract of C. occidentalis is an excellent candidate with potential antiproliferative activity against lung cancer; however, further studies are necessary to clarify its mechanism of action.http://dx.doi.org/10.1155/2023/6111058 |
| spellingShingle | Hanaa M. Sayed Mahmoud A. Ramadan Heba H. Salem Marwa A. A. Fayed Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma Journal of Chemistry |
| title | Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma |
| title_full | Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma |
| title_fullStr | Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma |
| title_full_unstemmed | Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma |
| title_short | Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma |
| title_sort | phytoconstituent isolation and cytotoxicity evaluation of the egyptian cassia occidentalis l possessing selective activity against lung carcinoma |
| url | http://dx.doi.org/10.1155/2023/6111058 |
| work_keys_str_mv | AT hanaamsayed phytoconstituentisolationandcytotoxicityevaluationoftheegyptiancassiaoccidentalislpossessingselectiveactivityagainstlungcarcinoma AT mahmoudaramadan phytoconstituentisolationandcytotoxicityevaluationoftheegyptiancassiaoccidentalislpossessingselectiveactivityagainstlungcarcinoma AT hebahsalem phytoconstituentisolationandcytotoxicityevaluationoftheegyptiancassiaoccidentalislpossessingselectiveactivityagainstlungcarcinoma AT marwaaafayed phytoconstituentisolationandcytotoxicityevaluationoftheegyptiancassiaoccidentalislpossessingselectiveactivityagainstlungcarcinoma |