Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice
Abstract Increases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils...
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| Format: | Article |
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BMC
2021-01-01
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| Series: | Laboratory Animal Research |
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| Online Access: | https://doi.org/10.1186/s42826-020-00080-y |
| _version_ | 1819278997070020608 |
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| author | Ae Ri Kim Yun Kyong Lim Joong-Ki Kook Eun-Jung Bak Yun-Jung Yoo |
| author_facet | Ae Ri Kim Yun Kyong Lim Joong-Ki Kook Eun-Jung Bak Yun-Jung Yoo |
| author_sort | Ae Ri Kim |
| collection | DOAJ |
| description | Abstract Increases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils. |
| first_indexed | 2024-12-24T00:20:53Z |
| format | Article |
| id | doaj.art-53187d6efc714860980c992f637e1062 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-24T00:20:53Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-53187d6efc714860980c992f637e10622022-12-21T17:24:35ZengBMCLaboratory Animal Research2233-76602021-01-013711710.1186/s42826-020-00080-yLipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of miceAe Ri Kim0Yun Kyong Lim1Joong-Ki Kook2Eun-Jung Bak3Yun-Jung Yoo4Department of Oral Biology, College of Dentistry, Yonsei UniversityKorean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun UniversityKorean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun UniversityDepartment of Oral Biology, College of Dentistry, Yonsei UniversityDepartment of Oral Biology, College of Dentistry, Yonsei UniversityAbstract Increases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils.https://doi.org/10.1186/s42826-020-00080-yPeriodontitisNeutrophilsRANKLPeriodontopathogen |
| spellingShingle | Ae Ri Kim Yun Kyong Lim Joong-Ki Kook Eun-Jung Bak Yun-Jung Yoo Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice Laboratory Animal Research Periodontitis Neutrophils RANKL Periodontopathogen |
| title | Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice |
| title_full | Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice |
| title_fullStr | Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice |
| title_full_unstemmed | Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice |
| title_short | Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice |
| title_sort | lipopolysaccharides of fusobacterium nucleatum and porphyromonas gingivalis increase rankl expressing neutrophils in air pouches of mice |
| topic | Periodontitis Neutrophils RANKL Periodontopathogen |
| url | https://doi.org/10.1186/s42826-020-00080-y |
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