RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation
26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytopla...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/34532 |
| _version_ | 1827767291754840064 |
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| author | Richard S Marshall Richard D Vierstra |
| author_facet | Richard S Marshall Richard D Vierstra |
| author_sort | Richard S Marshall |
| collection | DOAJ |
| description | 26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for granule assembly, not only influences the rate of proteasome degradation, but also impacts cell viability upon recovery from carbon starvation. PSG formation and concomitant protection against proteaphagy also occurs in Arabidopsis, suggesting that PSGs represent an evolutionarily conserved cache of proteasomes that can be rapidly re-mobilized based on energy availability. |
| first_indexed | 2024-03-11T11:56:41Z |
| format | Article |
| id | doaj.art-5319cfd817934fa4a30c32a98c2ee1f1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-11T11:56:41Z |
| publishDate | 2018-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5319cfd817934fa4a30c32a98c2ee1f12023-11-08T14:31:48ZengeLife Sciences Publications LtdeLife2050-084X2018-04-01710.7554/eLife.34532RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvationRichard S Marshall0https://orcid.org/0000-0002-6844-1078Richard D Vierstra1https://orcid.org/0000-0003-0210-3516Department of Biology, Washington University in St. Louis, St. Louis, United StatesDepartment of Biology, Washington University in St. Louis, St. Louis, United States26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for granule assembly, not only influences the rate of proteasome degradation, but also impacts cell viability upon recovery from carbon starvation. PSG formation and concomitant protection against proteaphagy also occurs in Arabidopsis, suggesting that PSGs represent an evolutionarily conserved cache of proteasomes that can be rapidly re-mobilized based on energy availability.https://elifesciences.org/articles/34532autophagyproteasomeproteaphagyproteasome storage granulesstarvationubiquitin |
| spellingShingle | Richard S Marshall Richard D Vierstra RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation eLife autophagy proteasome proteaphagy proteasome storage granules starvation ubiquitin |
| title | RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| title_full | RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| title_fullStr | RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| title_full_unstemmed | RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| title_short | RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| title_sort | retracted proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation |
| topic | autophagy proteasome proteaphagy proteasome storage granules starvation ubiquitin |
| url | https://elifesciences.org/articles/34532 |
| work_keys_str_mv | AT richardsmarshall retractedproteasomestoragegranulesprotectproteasomesfromautophagicdegradationuponcarbonstarvation AT richarddvierstra retractedproteasomestoragegranulesprotectproteasomesfromautophagicdegradationuponcarbonstarvation |