<b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70
The present study investigates the δ13C and δ15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentates. From each individual we extracted a strip of muscle from the region above the lateral line, in the dors...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2011-07-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7045 |
| _version_ | 1818247197004660736 |
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| author | Gislaine Iachstel Manetta Evanilde Benedito Carlos Ducatti |
| author_facet | Gislaine Iachstel Manetta Evanilde Benedito Carlos Ducatti |
| author_sort | Gislaine Iachstel Manetta |
| collection | DOAJ |
| description | The present study investigates the δ13C and δ15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentates. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze – control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for δ13C from the H. edentates samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish. |
| first_indexed | 2024-12-12T15:00:52Z |
| format | Article |
| id | doaj.art-531b7c1a77c847c29d6b7d997c009b93 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T15:00:52Z |
| publishDate | 2011-07-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-531b7c1a77c847c29d6b7d997c009b932022-12-22T00:20:48ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2011-07-0133410.4025/actascibiolsci.v33i4.7045<b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70Gislaine Iachstel Manetta0Evanilde Benedito1Carlos Ducatti2UEMUEM, MaringáUNESPThe present study investigates the δ13C and δ15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentates. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze – control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for δ13C from the H. edentates samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish.https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7045stable isotopepreservativefishesPlagioscion squamosissimusHypophthalmus edentatus |
| spellingShingle | Gislaine Iachstel Manetta Evanilde Benedito Carlos Ducatti <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 Acta Scientiarum: Biological Sciences stable isotope preservative fishes Plagioscion squamosissimus Hypophthalmus edentatus |
| title | <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 |
| title_full | <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 |
| title_fullStr | <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 |
| title_full_unstemmed | <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 |
| title_short | <b>Effect of alcohol and formaldehyde on the δ<sup>13</sup>C and δ<sup>15</sup>N isotopic composition of <em>Plagioscion squamosissimus</em> and <em>Hypophthalmus edentates</em> (Pisces, Osteichthyes)</b> - doi: 10.4025/actascibiolsci.v33i4.70 |
| title_sort | b effect of alcohol and formaldehyde on the delta sup 13 sup c and delta sup 15 sup n isotopic composition of em plagioscion squamosissimus em and em hypophthalmus edentates em pisces osteichthyes b doi 10 4025 actascibiolsci v33i4 70 |
| topic | stable isotope preservative fishes Plagioscion squamosissimus Hypophthalmus edentatus |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7045 |
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