Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil
ABSTRACT Background: The spread of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing gram-negative bacilli (GNB) represent a global public health threat that limits therapeutic options for hospitalized patients. This study aimed to evaluate the in-vitro susceptibility of β-lactam-re...
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| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2023-01-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100303&lng=en&tlng=en |
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| author | Thaisa Noceti Carvalho Vanessa Cristine Kobs Daniela Hille Roseneide Campos Deglmann Luiz Henrique Melo Paulo Henrique Condeixa de França |
| author_facet | Thaisa Noceti Carvalho Vanessa Cristine Kobs Daniela Hille Roseneide Campos Deglmann Luiz Henrique Melo Paulo Henrique Condeixa de França |
| author_sort | Thaisa Noceti Carvalho |
| collection | DOAJ |
| description | ABSTRACT Background: The spread of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing gram-negative bacilli (GNB) represent a global public health threat that limits therapeutic options for hospitalized patients. This study aimed to evaluate the in-vitro susceptibility of β-lactam-resistant GNB to ceftazidime-avibactam (C/A) and ceftolozane-tazobactam (C/T), and investigate the molecular determinants of resistance. Methods: Overall, 101 clinical isolates of Enterobacterales and Pseudomonas aeruginosa collected from a general hospital in Brazil were analyzed. Susceptibility to the antimicrobial agents was evaluated using an automated method, and the minimum inhibitory concentrations (MIC50/90) of C/A and C/T were determined using Etest®. The β-lactamase-encoding genes were investigated using polymerase chain reaction. Results: High susceptibility to C/A and C/T was observed among ESBL-producing Enterobacterales (100% and 97.3% for CLSI and 83.8% for BRCAST, respectively) and carbapenem-resistant P. aeruginosa (92.3% and 87.2%, respectively). Carbapenemase-producing Klebsiella pneumoniae exhibited high resistance to C/T (80%- CLSI or 100%- BRCAST) but high susceptibility to C/A (93.4%). All carbapenem-resistant K. pneumoniae isolates were susceptible to C/A, whereas only one isolate was susceptible to C/T. Both antimicrobials were inactive against metallo-β-lactamase-producing K. pneumoniae isolates. Resistance genes were concomitantly identified in 44 (44.9%) isolates, with bla CTX-M and bla SHV being the most common. Conclusions: C/A and C/T were active against microorganisms with β-lactam-resistant phenotypes, except when resistance was mediated by metallo-β-lactamases. Most C/A- and C/T-resistant isolates concomitantly carried two or more β-lactamase-encoding genes (62.5% and 77.4%, respectively). |
| first_indexed | 2024-04-10T20:45:45Z |
| format | Article |
| id | doaj.art-531b9daccdcb474c94e657041e67111e |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-10T20:45:45Z |
| publishDate | 2023-01-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-531b9daccdcb474c94e657041e67111e2023-01-24T07:35:32ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492023-01-015610.1590/0037-8682-0277-2022Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern BrazilThaisa Noceti Carvalhohttps://orcid.org/0000-0001-6273-7508Vanessa Cristine Kobshttps://orcid.org/0000-0001-6346-2278Daniela Hillehttps://orcid.org/0000-0003-2356-0054Roseneide Campos Deglmannhttps://orcid.org/0000-0001-5636-2703Luiz Henrique Melohttps://orcid.org/0000-0002-4461-6267Paulo Henrique Condeixa de Françahttps://orcid.org/0000-0002-1750-9132ABSTRACT Background: The spread of carbapenemase- and extended-spectrum β-lactamase (ESBL)-producing gram-negative bacilli (GNB) represent a global public health threat that limits therapeutic options for hospitalized patients. This study aimed to evaluate the in-vitro susceptibility of β-lactam-resistant GNB to ceftazidime-avibactam (C/A) and ceftolozane-tazobactam (C/T), and investigate the molecular determinants of resistance. Methods: Overall, 101 clinical isolates of Enterobacterales and Pseudomonas aeruginosa collected from a general hospital in Brazil were analyzed. Susceptibility to the antimicrobial agents was evaluated using an automated method, and the minimum inhibitory concentrations (MIC50/90) of C/A and C/T were determined using Etest®. The β-lactamase-encoding genes were investigated using polymerase chain reaction. Results: High susceptibility to C/A and C/T was observed among ESBL-producing Enterobacterales (100% and 97.3% for CLSI and 83.8% for BRCAST, respectively) and carbapenem-resistant P. aeruginosa (92.3% and 87.2%, respectively). Carbapenemase-producing Klebsiella pneumoniae exhibited high resistance to C/T (80%- CLSI or 100%- BRCAST) but high susceptibility to C/A (93.4%). All carbapenem-resistant K. pneumoniae isolates were susceptible to C/A, whereas only one isolate was susceptible to C/T. Both antimicrobials were inactive against metallo-β-lactamase-producing K. pneumoniae isolates. Resistance genes were concomitantly identified in 44 (44.9%) isolates, with bla CTX-M and bla SHV being the most common. Conclusions: C/A and C/T were active against microorganisms with β-lactam-resistant phenotypes, except when resistance was mediated by metallo-β-lactamases. Most C/A- and C/T-resistant isolates concomitantly carried two or more β-lactamase-encoding genes (62.5% and 77.4%, respectively).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100303&lng=en&tlng=enAntimicrobial resistanceGram-negative bacilliCeftazidime-avibactamCeftolozane-tazobactamIn vitro activityGenetic marker |
| spellingShingle | Thaisa Noceti Carvalho Vanessa Cristine Kobs Daniela Hille Roseneide Campos Deglmann Luiz Henrique Melo Paulo Henrique Condeixa de França Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil Revista da Sociedade Brasileira de Medicina Tropical Antimicrobial resistance Gram-negative bacilli Ceftazidime-avibactam Ceftolozane-tazobactam In vitro activity Genetic marker |
| title | Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil |
| title_full | Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil |
| title_fullStr | Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil |
| title_full_unstemmed | Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil |
| title_short | Evaluation of in-vitro susceptibility of ß-lactam-resistant Gram-negative bacilli to ceftazidime-avibactam and ceftolozane-tazobactam from clinical samples of a general hospital in southern Brazil |
| title_sort | evaluation of in vitro susceptibility of ss lactam resistant gram negative bacilli to ceftazidime avibactam and ceftolozane tazobactam from clinical samples of a general hospital in southern brazil |
| topic | Antimicrobial resistance Gram-negative bacilli Ceftazidime-avibactam Ceftolozane-tazobactam In vitro activity Genetic marker |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822023000100303&lng=en&tlng=en |
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