Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study
Abstract Background This study evaluated the efficacy of four methods in removing Candida albicans from the acrylic base material used to fabricate removable orthodontic appliances. Methods Heat-processed bars of orthodontic acrylic were incubated in a suspension of C. albicans for 2 h at 37 °C. Sam...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Oral Health |
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| Online Access: | http://link.springer.com/article/10.1186/s12903-019-0765-x |
| _version_ | 1819330704335437824 |
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| author | Abdul Razzak A. Ghazal Ghassan Idris Mohammad Y. Hajeer Karam Alawer Richard D. Cannon |
| author_facet | Abdul Razzak A. Ghazal Ghassan Idris Mohammad Y. Hajeer Karam Alawer Richard D. Cannon |
| author_sort | Abdul Razzak A. Ghazal |
| collection | DOAJ |
| description | Abstract Background This study evaluated the efficacy of four methods in removing Candida albicans from the acrylic base material used to fabricate removable orthodontic appliances. Methods Heat-processed bars of orthodontic acrylic were incubated in a suspension of C. albicans for 2 h at 37 °C. Samples were allocated into five groups (five bars per group) according to the cleaning method: (1) manual brushing using a toothbrush; (2) soaking in a commercial denture cleaning solution; (3) soaking in a commercial mouthwash solution; (4) using an ultrasonic cleaner; and (5) soaking in distilled water as a negative control. Yeast remaining attached to the bars after cleaning were removed by vortexing in growth medium and plated on Sabouraud dextrose agar. The reduction in yeast colony count after cleaning was calculated and expressed as the number of colony forming units per acrylic bar (CFU/bar). The experiment was carried out three times. Results All four cleaning methods resulted in a significant decrease in viable yeast cells associated with the acrylic bars compared to the control group. The mean percentage reduction in viable yeast cells affected by the cleaning methods was: brushing 89.9%; chlorhexidine 95.8%; ultrasonic cleaning 99.9%; and denture tablet 100%. Conclusions All four methods evaluated in this study were effective, to some extent, in removing C. albicans from orthodontic acrylic samples. The most effective, and readily available, cleaning method was the use of commercial denture sterilizing tablets. |
| first_indexed | 2024-12-24T14:02:45Z |
| format | Article |
| id | doaj.art-531d4fd89fbc45ecaab13ddd60294ba6 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-12-24T14:02:45Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-531d4fd89fbc45ecaab13ddd60294ba62022-12-21T16:52:25ZengBMCBMC Oral Health1472-68312019-05-011911710.1186/s12903-019-0765-xEfficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro studyAbdul Razzak A. Ghazal0Ghassan Idris1Mohammad Y. Hajeer2Karam Alawer3Richard D. Cannon4Technical Institution of Dentistry, University of HamaSir John Walsh Research Institute, University of OtagoDepartment of Orthodontics, University of Damascus Dental SchoolResearch microbiology Laboratory, Hama UniversitySir John Walsh Research Institute, University of OtagoAbstract Background This study evaluated the efficacy of four methods in removing Candida albicans from the acrylic base material used to fabricate removable orthodontic appliances. Methods Heat-processed bars of orthodontic acrylic were incubated in a suspension of C. albicans for 2 h at 37 °C. Samples were allocated into five groups (five bars per group) according to the cleaning method: (1) manual brushing using a toothbrush; (2) soaking in a commercial denture cleaning solution; (3) soaking in a commercial mouthwash solution; (4) using an ultrasonic cleaner; and (5) soaking in distilled water as a negative control. Yeast remaining attached to the bars after cleaning were removed by vortexing in growth medium and plated on Sabouraud dextrose agar. The reduction in yeast colony count after cleaning was calculated and expressed as the number of colony forming units per acrylic bar (CFU/bar). The experiment was carried out three times. Results All four cleaning methods resulted in a significant decrease in viable yeast cells associated with the acrylic bars compared to the control group. The mean percentage reduction in viable yeast cells affected by the cleaning methods was: brushing 89.9%; chlorhexidine 95.8%; ultrasonic cleaning 99.9%; and denture tablet 100%. Conclusions All four methods evaluated in this study were effective, to some extent, in removing C. albicans from orthodontic acrylic samples. The most effective, and readily available, cleaning method was the use of commercial denture sterilizing tablets.http://link.springer.com/article/10.1186/s12903-019-0765-xCandida albicans, Orthodontic acrylicOrthodontic appliancesDisinfection |
| spellingShingle | Abdul Razzak A. Ghazal Ghassan Idris Mohammad Y. Hajeer Karam Alawer Richard D. Cannon Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study BMC Oral Health Candida albicans, Orthodontic acrylic Orthodontic appliances Disinfection |
| title | Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study |
| title_full | Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study |
| title_fullStr | Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study |
| title_full_unstemmed | Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study |
| title_short | Efficacy of removing Candida albicans from orthodontic acrylic bases: an in vitro study |
| title_sort | efficacy of removing candida albicans from orthodontic acrylic bases an in vitro study |
| topic | Candida albicans, Orthodontic acrylic Orthodontic appliances Disinfection |
| url | http://link.springer.com/article/10.1186/s12903-019-0765-x |
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