Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells
<p>Abstract</p> <p>Background</p> <p><it>Chlamydiae </it>are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in <it>Yersi...
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| Format: | Article |
| Language: | English |
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BMC
2009-04-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/75 |
| _version_ | 1831522148237180928 |
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| author | Henriques-Normark Birgitta Normark Staffan Muschiol Sandra Subtil Agathe |
| author_facet | Henriques-Normark Birgitta Normark Staffan Muschiol Sandra Subtil Agathe |
| author_sort | Henriques-Normark Birgitta |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Chlamydiae </it>are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in <it>Yersinia</it>, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit <it>Chlamydia </it>growth in epithelial cells. In this study we have analyzed the effect of these drugs on bacterial invasiveness.</p> <p>Results</p> <p>We demonstrate that INPs affect <it>Chlamydia </it>growth in a dose dependent manner after bacterial invasion. The efficiency of <it>C. trachomatis </it>L2 and <it>C. caviae </it>GPIC entry into host cells was not altered in the presence of INPs. In <it>C. caviae</it>, entry appears to proceed normally with recruitment of actin and the small GTPases Rac, Cdc42 and Arf6 to the site of bacterial entry.</p> <p>Conclusion</p> <p>INPs have a strong inhibitory effect on <it>Chlamydia </it>growth. However, bacterial invasion is not altered in the presence of these drugs. In the light of these results, we discuss several hypotheses regarding the mode of action of INPs on type III secretion during the <it>Chlamydia </it>infectious cycle.</p> |
| first_indexed | 2024-12-14T01:27:01Z |
| format | Article |
| id | doaj.art-5321360ddb6e47cbbd79fad479731292 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T01:27:01Z |
| publishDate | 2009-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-5321360ddb6e47cbbd79fad4797312922022-12-21T23:22:10ZengBMCBMC Microbiology1471-21802009-04-01917510.1186/1471-2180-9-75Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cellsHenriques-Normark BirgittaNormark StaffanMuschiol SandraSubtil Agathe<p>Abstract</p> <p>Background</p> <p><it>Chlamydiae </it>are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in <it>Yersinia</it>, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit <it>Chlamydia </it>growth in epithelial cells. In this study we have analyzed the effect of these drugs on bacterial invasiveness.</p> <p>Results</p> <p>We demonstrate that INPs affect <it>Chlamydia </it>growth in a dose dependent manner after bacterial invasion. The efficiency of <it>C. trachomatis </it>L2 and <it>C. caviae </it>GPIC entry into host cells was not altered in the presence of INPs. In <it>C. caviae</it>, entry appears to proceed normally with recruitment of actin and the small GTPases Rac, Cdc42 and Arf6 to the site of bacterial entry.</p> <p>Conclusion</p> <p>INPs have a strong inhibitory effect on <it>Chlamydia </it>growth. However, bacterial invasion is not altered in the presence of these drugs. In the light of these results, we discuss several hypotheses regarding the mode of action of INPs on type III secretion during the <it>Chlamydia </it>infectious cycle.</p>http://www.biomedcentral.com/1471-2180/9/75 |
| spellingShingle | Henriques-Normark Birgitta Normark Staffan Muschiol Sandra Subtil Agathe Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells BMC Microbiology |
| title | Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells |
| title_full | Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells |
| title_fullStr | Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells |
| title_full_unstemmed | Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells |
| title_short | Small molecule inhibitors of the <it>Yersinia </it>type III secretion system impair the development of <it>Chlamydia </it>after entry into host cells |
| title_sort | small molecule inhibitors of the it yersinia it type iii secretion system impair the development of it chlamydia it after entry into host cells |
| url | http://www.biomedcentral.com/1471-2180/9/75 |
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