Analysis of conditions for the preparation of BSA/MMT composites
This study describes a new method for protein fixation that has high, stable, and good repeatable fixation effect without deactivating protein. This method involves intercalating bovine serum albumin (BSA) into montmorillonite (MMT) under different experimental conditions, i.e., the concentration of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2013-08-01
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| Series: | Science and Engineering of Composite Materials |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/secm-2012-0073 |
| _version_ | 1818666751339003904 |
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| author | Lv Yinghai Su Xiaolu Ren Fuli Kan Hong-qiang Kong Ying |
| author_facet | Lv Yinghai Su Xiaolu Ren Fuli Kan Hong-qiang Kong Ying |
| author_sort | Lv Yinghai |
| collection | DOAJ |
| description | This study describes a new method for protein fixation that has high, stable, and good repeatable fixation effect without deactivating protein. This method involves intercalating bovine serum albumin (BSA) into montmorillonite (MMT) under different experimental conditions, i.e., the concentration of coupling agent KH570, pH values, ionic strength, and the impact of buffers. The study showed the interaction of buffer systems with pH values had the most significant influence on adsorption quantity. The existence of the buffer increased the adsorption quantity and helped with slow protein releasing, while the phosphate buffer worked better than acetate buffer. More BSA was adsorbed on KH570 treated than untreated MMT, and among the treated samples the most protein released was on MMT treated with KH570 in 100% ethanol and in the acetate buffer solution. Adsorption quantity of the BSA initially increased and then decreased with the increase of the ionic strength. The research on the preparation of MMT/BSA materials provides further information on the study of protein composites. |
| first_indexed | 2024-12-17T06:09:30Z |
| format | Article |
| id | doaj.art-5323502962ff4f7fba2c8f7cb7081be2 |
| institution | Directory Open Access Journal |
| issn | 0792-1233 2191-0359 |
| language | English |
| last_indexed | 2024-12-17T06:09:30Z |
| publishDate | 2013-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Science and Engineering of Composite Materials |
| spelling | doaj.art-5323502962ff4f7fba2c8f7cb7081be22022-12-21T22:00:41ZengDe GruyterScience and Engineering of Composite Materials0792-12332191-03592013-08-0120322122610.1515/secm-2012-0073Analysis of conditions for the preparation of BSA/MMT compositesLv YinghaiSu Xiaolu0Ren Fuli1Kan Hong-qiang2Kong Ying3School of Medical Lab Science and Life Sciences, WenZhou Medical College, Wenzhou, ChinaCollege of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, ChinaCollege of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, ChinaState Key Laboratory of Heavy Oil Processing, China University of Petroleum (Huadong), Qingdao, Shandong, 266580, ChinaThis study describes a new method for protein fixation that has high, stable, and good repeatable fixation effect without deactivating protein. This method involves intercalating bovine serum albumin (BSA) into montmorillonite (MMT) under different experimental conditions, i.e., the concentration of coupling agent KH570, pH values, ionic strength, and the impact of buffers. The study showed the interaction of buffer systems with pH values had the most significant influence on adsorption quantity. The existence of the buffer increased the adsorption quantity and helped with slow protein releasing, while the phosphate buffer worked better than acetate buffer. More BSA was adsorbed on KH570 treated than untreated MMT, and among the treated samples the most protein released was on MMT treated with KH570 in 100% ethanol and in the acetate buffer solution. Adsorption quantity of the BSA initially increased and then decreased with the increase of the ionic strength. The research on the preparation of MMT/BSA materials provides further information on the study of protein composites.https://doi.org/10.1515/secm-2012-0073bovine serum albumincompositeconditionmontmorillonite |
| spellingShingle | Lv Yinghai Su Xiaolu Ren Fuli Kan Hong-qiang Kong Ying Analysis of conditions for the preparation of BSA/MMT composites Science and Engineering of Composite Materials bovine serum albumin composite condition montmorillonite |
| title | Analysis of conditions for the preparation of BSA/MMT composites |
| title_full | Analysis of conditions for the preparation of BSA/MMT composites |
| title_fullStr | Analysis of conditions for the preparation of BSA/MMT composites |
| title_full_unstemmed | Analysis of conditions for the preparation of BSA/MMT composites |
| title_short | Analysis of conditions for the preparation of BSA/MMT composites |
| title_sort | analysis of conditions for the preparation of bsa mmt composites |
| topic | bovine serum albumin composite condition montmorillonite |
| url | https://doi.org/10.1515/secm-2012-0073 |
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