A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device
A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-ef...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/6/869 |
| _version_ | 1797484328472543232 |
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| author | Chengzhuang Yu Shanshan Li Chunyang Wei Shijie Dai Xinyi Liang Junwei Li |
| author_facet | Chengzhuang Yu Shanshan Li Chunyang Wei Shijie Dai Xinyi Liang Junwei Li |
| author_sort | Chengzhuang Yu |
| collection | DOAJ |
| description | A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-effective fluorescence microscope. Assembled from a set of 3D print components and a webcam, it consists of a three-degree-of-freedom sliding platform and a microscopic imaging system. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. The resolution and field of view of the microscope were evaluated. Compared with a laboratory-grade inverted fluorescence microscope, the portable microscope shows satisfactory performance, both in the bright-field and fluorescence mode. From the configurations of local resources, the microscope costs around USD 100 to assemble. To demonstrate the capability of the portable fluorescence microscope, we proposed a quantitative polymerase chain reaction experiment for meat product authenticating applications. The portable and low-cost microscope platform demonstrates the benefits in space-constrained environments and shows high potential in telemedicine, point-of-care testing, and more. |
| first_indexed | 2024-03-09T23:01:55Z |
| format | Article |
| id | doaj.art-5326565da1b248a2b2e44265ff56d230 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T23:01:55Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-5326565da1b248a2b2e44265ff56d2302023-11-23T18:00:43ZengMDPI AGMicromachines2072-666X2022-05-0113686910.3390/mi13060869A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic DeviceChengzhuang Yu0Shanshan Li1Chunyang Wei2Shijie Dai3Xinyi Liang4Junwei Li5Hebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, ChinaHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, ChinaHebei Key Laboratory of Smart Sensing and Human-Robot Interactions, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, ChinaState Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, ChinaInstitute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300130, ChinaInstitute of Biophysics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin 300130, ChinaA fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-effective fluorescence microscope. Assembled from a set of 3D print components and a webcam, it consists of a three-degree-of-freedom sliding platform and a microscopic imaging system. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. The resolution and field of view of the microscope were evaluated. Compared with a laboratory-grade inverted fluorescence microscope, the portable microscope shows satisfactory performance, both in the bright-field and fluorescence mode. From the configurations of local resources, the microscope costs around USD 100 to assemble. To demonstrate the capability of the portable fluorescence microscope, we proposed a quantitative polymerase chain reaction experiment for meat product authenticating applications. The portable and low-cost microscope platform demonstrates the benefits in space-constrained environments and shows high potential in telemedicine, point-of-care testing, and more.https://www.mdpi.com/2072-666X/13/6/869portablefluorescencemicroscopemeat products authenticating |
| spellingShingle | Chengzhuang Yu Shanshan Li Chunyang Wei Shijie Dai Xinyi Liang Junwei Li A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device Micromachines portable fluorescence microscope meat products authenticating |
| title | A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device |
| title_full | A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device |
| title_fullStr | A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device |
| title_full_unstemmed | A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device |
| title_short | A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device |
| title_sort | cost effective nucleic acid detection system using a portable microscopic device |
| topic | portable fluorescence microscope meat products authenticating |
| url | https://www.mdpi.com/2072-666X/13/6/869 |
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