Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products
Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms. Sequencing technologies have revealed that the number of biosynthetic gene clusters (BGCs) in marine microo...
| Những tác giả chính: | , , , , , , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
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MDPI AG
2022-05-01
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| Loạt: | Marine Drugs |
| Những chủ đề: | |
| Truy cập trực tuyến: | https://www.mdpi.com/1660-3397/20/6/341 |
| _version_ | 1827659011211657216 |
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| author | Yushan Xu Xinhua Du Xionghui Yu Qian Jiang Kaiwen Zheng Jinzhong Xu Pinmei Wang |
| author_facet | Yushan Xu Xinhua Du Xionghui Yu Qian Jiang Kaiwen Zheng Jinzhong Xu Pinmei Wang |
| author_sort | Yushan Xu |
| collection | DOAJ |
| description | Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms. Sequencing technologies have revealed that the number of biosynthetic gene clusters (BGCs) in marine microorganisms and the marine environment is much higher than expected. Unfortunately, the majority of them are silent or only weakly expressed under traditional laboratory culture conditions. Furthermore, the large proportion of marine microorganisms are either uncultivable or cannot be genetically manipulated. Efficient heterologous expression systems can activate cryptic BGCs and increase target compound yield, allowing researchers to explore more unknown MNPs. When developing heterologous expression of MNPs, it is critical to consider heterologous host selection as well as genetic manipulations for BGCs. In this review, we summarize current progress on the heterologous expression of MNPs as a reference for future research. |
| first_indexed | 2024-03-09T23:13:35Z |
| format | Article |
| id | doaj.art-5326625d2c054b8ea59791046dc38fee |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T23:13:35Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-5326625d2c054b8ea59791046dc38fee2023-11-23T17:40:34ZengMDPI AGMarine Drugs1660-33972022-05-0120634110.3390/md20060341Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural ProductsYushan Xu0Xinhua Du1Xionghui Yu2Qian Jiang3Kaiwen Zheng4Jinzhong Xu5Pinmei Wang6Ocean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaMarine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms. Sequencing technologies have revealed that the number of biosynthetic gene clusters (BGCs) in marine microorganisms and the marine environment is much higher than expected. Unfortunately, the majority of them are silent or only weakly expressed under traditional laboratory culture conditions. Furthermore, the large proportion of marine microorganisms are either uncultivable or cannot be genetically manipulated. Efficient heterologous expression systems can activate cryptic BGCs and increase target compound yield, allowing researchers to explore more unknown MNPs. When developing heterologous expression of MNPs, it is critical to consider heterologous host selection as well as genetic manipulations for BGCs. In this review, we summarize current progress on the heterologous expression of MNPs as a reference for future research.https://www.mdpi.com/1660-3397/20/6/341marine natural productsheterologous expressionheterologous hostsbiosynthetic gene clustersgenetic manipulation |
| spellingShingle | Yushan Xu Xinhua Du Xionghui Yu Qian Jiang Kaiwen Zheng Jinzhong Xu Pinmei Wang Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products Marine Drugs marine natural products heterologous expression heterologous hosts biosynthetic gene clusters genetic manipulation |
| title | Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products |
| title_full | Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products |
| title_fullStr | Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products |
| title_full_unstemmed | Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products |
| title_short | Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products |
| title_sort | recent advances in the heterologous expression of biosynthetic gene clusters for marine natural products |
| topic | marine natural products heterologous expression heterologous hosts biosynthetic gene clusters genetic manipulation |
| url | https://www.mdpi.com/1660-3397/20/6/341 |
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