Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response
The plant response to stresses includes changes in starch metabolism regulated by a complex catalytic network, in which plastidic starch phosphorylase PHO1a is one of the key players. In this study, we used the CRISPR-Cas9 system to edit the <i>PHO1a</i> gene in four potato (<i>Sol...
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MDPI AG
2022-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/8/730 |
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| author | Anna V. Nezhdanova Gleb I. Efremov Maria A. Slugina Anastasia M. Kamionskaya Elena Z. Kochieva Anna V. Shchennikova |
| author_facet | Anna V. Nezhdanova Gleb I. Efremov Maria A. Slugina Anastasia M. Kamionskaya Elena Z. Kochieva Anna V. Shchennikova |
| author_sort | Anna V. Nezhdanova |
| collection | DOAJ |
| description | The plant response to stresses includes changes in starch metabolism regulated by a complex catalytic network, in which plastidic starch phosphorylase PHO1a is one of the key players. In this study, we used the CRISPR-Cas9 system to edit the <i>PHO1a</i> gene in four potato (<i>Solanum tuberosum</i> L.) cultivars, which resulted in the introduction of a radical mutation, G261V, into the PHO1a functional domain. The mutants had altered morphology and differed from wild-type plants in starch content in the roots and leaves. Exposure to cold stress revealed the differential response of parental and transgenic plants in terms of starch content and the expression of genes coding for β-amylases, amylase inhibitors, and stress-responsive MADS-domain transcription factors. These results suggest that the G261V mutation causes changes in the functional activity of PHO1a, which in turn affect the coordinated operation of starch catabolism enzymes both under normal and cold stress conditions, possibly through differential expression of MADS-domain transcription factors. Our results highlight a critical regulatory role of PHO1a in starch metabolism, root and shoot development, and stress response in potatoes. |
| first_indexed | 2024-03-09T09:56:17Z |
| format | Article |
| id | doaj.art-5327787def5a432cbc8ae413456090a6 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T09:56:17Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-5327787def5a432cbc8ae413456090a62023-12-01T23:46:06ZengMDPI AGHorticulturae2311-75242022-08-018873010.3390/horticulturae8080730Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress ResponseAnna V. Nezhdanova0Gleb I. Efremov1Maria A. Slugina2Anastasia M. Kamionskaya3Elena Z. Kochieva4Anna V. Shchennikova5Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, 119071 Moscow, RussiaThe plant response to stresses includes changes in starch metabolism regulated by a complex catalytic network, in which plastidic starch phosphorylase PHO1a is one of the key players. In this study, we used the CRISPR-Cas9 system to edit the <i>PHO1a</i> gene in four potato (<i>Solanum tuberosum</i> L.) cultivars, which resulted in the introduction of a radical mutation, G261V, into the PHO1a functional domain. The mutants had altered morphology and differed from wild-type plants in starch content in the roots and leaves. Exposure to cold stress revealed the differential response of parental and transgenic plants in terms of starch content and the expression of genes coding for β-amylases, amylase inhibitors, and stress-responsive MADS-domain transcription factors. These results suggest that the G261V mutation causes changes in the functional activity of PHO1a, which in turn affect the coordinated operation of starch catabolism enzymes both under normal and cold stress conditions, possibly through differential expression of MADS-domain transcription factors. Our results highlight a critical regulatory role of PHO1a in starch metabolism, root and shoot development, and stress response in potatoes.https://www.mdpi.com/2311-7524/8/8/730starch phosphorylase PHO1apotato <i>Solanum tuberosum</i> L.CRISPR-Cas9 editingstarch metabolism |
| spellingShingle | Anna V. Nezhdanova Gleb I. Efremov Maria A. Slugina Anastasia M. Kamionskaya Elena Z. Kochieva Anna V. Shchennikova Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response Horticulturae starch phosphorylase PHO1a potato <i>Solanum tuberosum</i> L. CRISPR-Cas9 editing starch metabolism |
| title | Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response |
| title_full | Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response |
| title_fullStr | Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response |
| title_full_unstemmed | Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response |
| title_short | Effect of a Radical Mutation in Plastidic Starch Phosphorylase PHO1a on Potato Growth and Cold Stress Response |
| title_sort | effect of a radical mutation in plastidic starch phosphorylase pho1a on potato growth and cold stress response |
| topic | starch phosphorylase PHO1a potato <i>Solanum tuberosum</i> L. CRISPR-Cas9 editing starch metabolism |
| url | https://www.mdpi.com/2311-7524/8/8/730 |
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