MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function
Summary: We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 μg/ml) for 24 h coor...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223004169 |
| _version_ | 1797867380068581376 |
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| author | Laura Hume Jessica C. Edge Haidee Tinning Dapeng T. Wang Alysha S. Taylor Vladimir Ovchinnikov Annika V. Geijer-Simpson Pavle Vrljicak Jan J. Brosens Emma S. Lucas Nigel A.B. Simpson Jayne Shillito Karen Forbes Mary J. O’Connell Niamh Forde |
| author_facet | Laura Hume Jessica C. Edge Haidee Tinning Dapeng T. Wang Alysha S. Taylor Vladimir Ovchinnikov Annika V. Geijer-Simpson Pavle Vrljicak Jan J. Brosens Emma S. Lucas Nigel A.B. Simpson Jayne Shillito Karen Forbes Mary J. O’Connell Niamh Forde |
| author_sort | Laura Hume |
| collection | DOAJ |
| description | Summary: We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 μg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss. |
| first_indexed | 2024-04-09T23:39:19Z |
| format | Article |
| id | doaj.art-53288b4ef1ff4e27859d5eecc43cebec |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T23:39:19Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-53288b4ef1ff4e27859d5eecc43cebec2023-03-19T04:38:21ZengElsevieriScience2589-00422023-04-01264106339MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial functionLaura Hume0Jessica C. Edge1Haidee Tinning2Dapeng T. Wang3Alysha S. Taylor4Vladimir Ovchinnikov5Annika V. Geijer-Simpson6Pavle Vrljicak7Jan J. Brosens8Emma S. Lucas9Nigel A.B. Simpson10Jayne Shillito11Karen Forbes12Mary J. O’Connell13Niamh Forde14Discovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UKLeedsOmics, University of Leeds, Leeds, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UK; School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UKSchool of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, NG7 2RD, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UK; School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UKDivision of Biomedical Sciences, Clinical Sciences Research Laboratories, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UKDivision of Biomedical Sciences, Clinical Sciences Research Laboratories, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK; Tommy’s National Centre for Miscarriage Research, University Hospital Coventry and Warwickshire, Coventry CV2 2DX, UKDivision of Biomedical Sciences, Clinical Sciences Research Laboratories, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UKDepartment of Women’s and Children’s Health, School of Medicine, University of Leeds, Leeds LS2 9JT, UK; Leeds Teaching Hospitals Trust, Beckett St, Leeds LS9 7TF, UKLeeds Teaching Hospitals Trust, Beckett St, Leeds LS9 7TF, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UKSchool of Life Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, NG7 2RD, UKDiscovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, Faculty of Medicine and Health, University of Leeds, Leeds, LS2 9JT, UK; Corresponding authorSummary: We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 μg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss.http://www.sciencedirect.com/science/article/pii/S2589004223004169Biological sciencesMolecular biologyDevelopmental biologyEmbryology |
| spellingShingle | Laura Hume Jessica C. Edge Haidee Tinning Dapeng T. Wang Alysha S. Taylor Vladimir Ovchinnikov Annika V. Geijer-Simpson Pavle Vrljicak Jan J. Brosens Emma S. Lucas Nigel A.B. Simpson Jayne Shillito Karen Forbes Mary J. O’Connell Niamh Forde MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function iScience Biological sciences Molecular biology Developmental biology Embryology |
| title | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| title_full | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| title_fullStr | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| title_full_unstemmed | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| title_short | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| title_sort | micrornas emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
| topic | Biological sciences Molecular biology Developmental biology Embryology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223004169 |
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