Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs
β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by Malbranchea pulchella was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of de...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X21000345 |
| _version_ | 1818888606291329024 |
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| author | Lummy Maria Oliveira Monteiro Ana Claudia Vici Josana Maria Messias Paulo Ricardo Heinen Vanessa Elisa Pinheiro Carem Gledes Vargas Rechia Marcos S. Buckeridge Maria de Lourdes Teixeira de Moraes Polizeli |
| author_facet | Lummy Maria Oliveira Monteiro Ana Claudia Vici Josana Maria Messias Paulo Ricardo Heinen Vanessa Elisa Pinheiro Carem Gledes Vargas Rechia Marcos S. Buckeridge Maria de Lourdes Teixeira de Moraes Polizeli |
| author_sort | Lummy Maria Oliveira Monteiro |
| collection | DOAJ |
| description | β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by Malbranchea pulchella was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of determination (R2) was 0.9960, F-value was very high, and the lack of fit was found to be non-significant. This indicates a statistic valid and predictive result. M. pulchella enzymatic extract was successfully tested as an enzymatic cocktail in a mixture design using sugarcane bagasse, soybean hull and barley bagasse. We proved that the optimization of the β-glucosidase production and the application in hydrolysis using unexpansive biomass and agricultural wastes can be accomplished by means of statistical methodologies. The strategy presented here can be useful for the improvement of enzyme production and the hydrolysis process, arising as an alternative for bioeconomy. |
| first_indexed | 2024-12-19T16:55:48Z |
| format | Article |
| id | doaj.art-5328a2e1240e49398389e52918ab3ba0 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-19T16:55:48Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-5328a2e1240e49398389e52918ab3ba02022-12-21T20:13:25ZengElsevierBiotechnology Reports2215-017X2021-06-0130e00618Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designsLummy Maria Oliveira Monteiro0Ana Claudia Vici1Josana Maria Messias2Paulo Ricardo Heinen3Vanessa Elisa Pinheiro4Carem Gledes Vargas Rechia5Marcos S. Buckeridge6Maria de Lourdes Teixeira de Moraes Polizeli7Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14049-900, Ribeirão Preto, SP, BrazilFaculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-900, Ribeirão Preto, SP, BrazilFaculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-900, Ribeirão Preto, SP, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14049-900, Ribeirão Preto, SP, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14049-900, Ribeirão Preto, SP, BrazilFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-903, Ribeirão Preto, SP, BrazilInstituto de Biociências, Universidade de São Paulo, Matão Street, 277, 05508-090, São Paulo, SP, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14049-900, Ribeirão Preto, SP, Brazil; Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-900, Ribeirão Preto, SP, Brazil; Corresponding author at: Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-900, Ribeirão Preto, São Paulo, Brazil.β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by Malbranchea pulchella was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of determination (R2) was 0.9960, F-value was very high, and the lack of fit was found to be non-significant. This indicates a statistic valid and predictive result. M. pulchella enzymatic extract was successfully tested as an enzymatic cocktail in a mixture design using sugarcane bagasse, soybean hull and barley bagasse. We proved that the optimization of the β-glucosidase production and the application in hydrolysis using unexpansive biomass and agricultural wastes can be accomplished by means of statistical methodologies. The strategy presented here can be useful for the improvement of enzyme production and the hydrolysis process, arising as an alternative for bioeconomy.http://www.sciencedirect.com/science/article/pii/S2215017X21000345Thermophilic fungusSecond-generation ethanolComposite central designMixture design |
| spellingShingle | Lummy Maria Oliveira Monteiro Ana Claudia Vici Josana Maria Messias Paulo Ricardo Heinen Vanessa Elisa Pinheiro Carem Gledes Vargas Rechia Marcos S. Buckeridge Maria de Lourdes Teixeira de Moraes Polizeli Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs Biotechnology Reports Thermophilic fungus Second-generation ethanol Composite central design Mixture design |
| title | Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs |
| title_full | Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs |
| title_fullStr | Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs |
| title_full_unstemmed | Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs |
| title_short | Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs |
| title_sort | increased malbranchea pulchella β glucosidase production and its application in agroindustrial residue hydrolysis a research based on experimental designs |
| topic | Thermophilic fungus Second-generation ethanol Composite central design Mixture design |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X21000345 |
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