In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i>
<i>Trypanosoma cruzi</i> is a protozoan parasite responsible for Chagas disease, which affects millions around the world and is not treatable in its chronic stage. Sodium diethyldithiocarbamate is a compound belonging to the carbamate class and, in a previous study, demonstrated high eff...
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/497 |
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| author | Johny Wysllas de Freitas Oliveira Mariana Farias Alves da Silva Igor Zumba Damasceno Hugo Alexandre Oliveira Rocha Arnóbio Antônio da Silva Júnior Marcelo Sousa Silva |
| author_facet | Johny Wysllas de Freitas Oliveira Mariana Farias Alves da Silva Igor Zumba Damasceno Hugo Alexandre Oliveira Rocha Arnóbio Antônio da Silva Júnior Marcelo Sousa Silva |
| author_sort | Johny Wysllas de Freitas Oliveira |
| collection | DOAJ |
| description | <i>Trypanosoma cruzi</i> is a protozoan parasite responsible for Chagas disease, which affects millions around the world and is not treatable in its chronic stage. Sodium diethyldithiocarbamate is a compound belonging to the carbamate class and, in a previous study, demonstrated high efficacy against <i>T. cruzi</i>, showing itself to be a promising compound for the treatment of Chagas disease. This study investigates the encapsulation of sodium diethyldithiocarbamate by poly-lactic acid in nanoparticles, a system of biodegradable nanoparticles that is capable of reducing the toxicity caused by free DETC against cells and maintaining the antiparasitic activity. The nanosystem PLA-DETC was fabricated using nanoprecipitation, and its physical characterization was measured via DLS, SEM, and AFM, demonstrating a small size around 168 nm and a zeta potential of around −19 mv. Furthermore, the toxicity was determined by MTT reduction against three cell lines (VERO, 3T3, and RAW), and when compared to free DETC, we observed a reduction in cell mortality, demonstrating the importance of DETC nanoencapsulation. In addition, the nanoparticles were stained with FITC and put in contact with cells for 24 h, followed by confirmation of whether the nanosystem was inside the cells. Lastly, the antiparasitic activity against different strains of <i>T. cruzi</i> in trypomastigote forms was determined by resazurin reduction and ROS production, which demonstrated high efficacy towards <i>T. cruzi</i> equal to that of free DETC. |
| first_indexed | 2024-03-09T12:59:43Z |
| format | Article |
| id | doaj.art-532d426cddbd494a889547df8db8d112 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:59:43Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-532d426cddbd494a889547df8db8d1122023-11-30T21:55:44ZengMDPI AGPharmaceutics1999-49232022-02-0114349710.3390/pharmaceutics14030497In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i>Johny Wysllas de Freitas Oliveira0Mariana Farias Alves da Silva1Igor Zumba Damasceno2Hugo Alexandre Oliveira Rocha3Arnóbio Antônio da Silva Júnior4Marcelo Sousa Silva5Immunoparasitology Laboratory, Department of Clinical and Toxicological Analysis, Centre of Health Sciences, Federal University of Rio Grande do Norte, Natal 59012-570, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte—UFRN, Natal 59012-570, BrazilDepartamento de Engenharia de Materiais, Department of Engineer, Centro de Tecnologia, Universidade Federal do Rio Grande do Norte, Natal 59078-970, BrazilPrograma de Pós-Graduação em Bioquímica, Department of Biochemistry, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal 59078-970, BrazilLaboratory of Pharmaceutical Technology and Biotechnology, Department of Pharmacy, Federal University of Rio Grande do Norte—UFRN, Natal 59012-570, BrazilImmunoparasitology Laboratory, Department of Clinical and Toxicological Analysis, Centre of Health Sciences, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil<i>Trypanosoma cruzi</i> is a protozoan parasite responsible for Chagas disease, which affects millions around the world and is not treatable in its chronic stage. Sodium diethyldithiocarbamate is a compound belonging to the carbamate class and, in a previous study, demonstrated high efficacy against <i>T. cruzi</i>, showing itself to be a promising compound for the treatment of Chagas disease. This study investigates the encapsulation of sodium diethyldithiocarbamate by poly-lactic acid in nanoparticles, a system of biodegradable nanoparticles that is capable of reducing the toxicity caused by free DETC against cells and maintaining the antiparasitic activity. The nanosystem PLA-DETC was fabricated using nanoprecipitation, and its physical characterization was measured via DLS, SEM, and AFM, demonstrating a small size around 168 nm and a zeta potential of around −19 mv. Furthermore, the toxicity was determined by MTT reduction against three cell lines (VERO, 3T3, and RAW), and when compared to free DETC, we observed a reduction in cell mortality, demonstrating the importance of DETC nanoencapsulation. In addition, the nanoparticles were stained with FITC and put in contact with cells for 24 h, followed by confirmation of whether the nanosystem was inside the cells. Lastly, the antiparasitic activity against different strains of <i>T. cruzi</i> in trypomastigote forms was determined by resazurin reduction and ROS production, which demonstrated high efficacy towards <i>T. cruzi</i> equal to that of free DETC.https://www.mdpi.com/1999-4923/14/3/497sodium diethyldithiocarbamate<i>Trypanosoma cruzi</i>Chagas diseasenanoparticlenanoprecipitation |
| spellingShingle | Johny Wysllas de Freitas Oliveira Mariana Farias Alves da Silva Igor Zumba Damasceno Hugo Alexandre Oliveira Rocha Arnóbio Antônio da Silva Júnior Marcelo Sousa Silva In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> Pharmaceutics sodium diethyldithiocarbamate <i>Trypanosoma cruzi</i> Chagas disease nanoparticle nanoprecipitation |
| title | In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> |
| title_full | In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> |
| title_fullStr | In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> |
| title_full_unstemmed | In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> |
| title_short | In Vitro Validation of Antiparasitic Activity of PLA-Nanoparticles of Sodium Diethyldithiocarbamate against <i>Trypanosoma cruzi</i> |
| title_sort | in vitro validation of antiparasitic activity of pla nanoparticles of sodium diethyldithiocarbamate against i trypanosoma cruzi i |
| topic | sodium diethyldithiocarbamate <i>Trypanosoma cruzi</i> Chagas disease nanoparticle nanoprecipitation |
| url | https://www.mdpi.com/1999-4923/14/3/497 |
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